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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pBOB-synP-HT
 
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RRID:Addgene_30456 histone GFP Ampicillin Backbone Marker:Addgene plasmid 12337; Backbone Size:8000; Vector Backbone:pCSC-SP-PW-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-07-25 12:45:37 6
pCS2-F1-GCC
 
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RRID:Addgene_30698 F1 GCC Danio rerio Ampicillin PMID:21937602 Backbone Size:6141; Vector Backbone:pCS2; Vector Types:mRNA production; Bacterial Resistance:Ampicillin 2026-07-25 12:45:38 0
pBS-F1-AAG
 
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RRID:Addgene_30687 F1 AAG Ampicillin PMID:21937602 Backbone Size:2877; Vector Backbone:pBlueScript II SK(+); Vector Types:Cloning vector; Bacterial Resistance:Ampicillin 2026-07-25 12:45:38 0
MyD116.delC.pBABEpu
 
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RRID:Addgene_21835 GADD34 Mus musculus Ampicillin PMID:11381086 The construct was created by recovering the Sma1 fragment of mMyD116 and ligating it into the unique SnB1 site of pBABEpu. The stop codo is deep in the downstream SV40 promoter sequence andowing the encoded poly-peptide with a tail of nonesense sequence, but it does serve as an expressed negative control for the effects of GADD34. Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-07-25 12:44:28 1
MyD116.PFLAG.CMV2
 
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RRID:Addgene_21834 GADD34 Mus musculus Ampicillin PMID:11381086 Backbone Size:4724; Vector Backbone:pCMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:44:28 1
XBP-1 2: pFLAG.XBP1p.CMV2
 
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10+ mentions
RRID:Addgene_21833 XBP-1 processed/spliced Mus musculus Ampicillin PMID:11780124 Addgene sequencing results show two mismatches with author's sequence causing two amino acid mutations - G26V and A76V. FLAG-epitope tagged IRE1-spliced XBP-1 in the mammalian expression plasmid pFLAG-CMV-2. Flag-tagged version of XBP-1 was constructed by inserting the Flag epitope tag sequence immediately 3' of the ATG start codon. Note: Cheah Kathryn Song Eng reported on November 4, 2004 that the sequence of this plasmid deviates from the predicted by G1028T. This impacts on the CDS changing Gly27Val. We do not know if this is a mutation that crept into our construction, a mutation that was present in the cDNA we purchased or if it is a point in which the mouse genome has some variability. Nor do we know the functional significance of this, but we note that the corresponding residue in the human is Ala. Furthermore, this variation/mutation is in a region of relatively poor sequence conservation across species, suggesting that it might not be all that important. Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:44:28 14
XBP-1 1: pFLAG.XBP1u.CMV2
 
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RRID:Addgene_21832 XBP-1 unprocessed Mus musculus Ampicillin PMID:11780124 Addgene sequencing results show two mismatches with author's sequence causing two amino acid mutations - G27V and A76V. FLAG-epitope tagged unspliced XBP-1 in the mammalian expression plasmid pFLAG-CMV-2. Flag-tagged version of XBP-1 was constructed by inserting the Flag epitope tag sequence immediately 3' of the ATG start codon. Note: Cheah Kathryn Song Eng reported on November 4, 2004 that the sequence of this plasmid deviates from the predicted by G1028T. This impacts on the CDS changing Gly27Val. We do not know if this is a mutation that crept into our construction, a mutation that was present in the cDNA we purchased or if it is a point in which the mouse genome has some variability. Nor do we know the functional significance of this, but we note that the corresponding residue in the human is Ala. Furthermore, this variation/mutation is in a region of relatively poor sequence conservation across species, suggesting that it might not be all that important. Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:44:28 5
AIRAP 8: pCEFL.PSMB2.GST
 
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RRID:Addgene_21798 PSMB2 Mus musculus Ampicillin PMID:16973439 Backbone Size:5921; Vector Backbone:pCEFL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:44:27 0
TLS 4: hTLS-CHOP.pBSKS(+)
 
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RRID:Addgene_21830 TLS-CHOP Homo sapiens Ampicillin PMID:8510758 Human TLS-CHOP fusion oncogene full-length cDNA in pBS KS+ cloning vector Insert between BamH1 and Xho1. A human CHOP cDNA probe can be excised from this plasmid by Pst1-Xho1 digestion. Backbone Marker:Stratagene; Backbone Size:2900; Vector Backbone:pBluescript KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:44:28 0
AIRAP 6: AIRAP(1-89).GST,V1.pcDNA3
 
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RRID:Addgene_21796 AIRAP Mus musculus Ampicillin PMID:16973439 Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin AA 1-89 2026-07-25 12:44:27 0
AIRAP 5: AIRAP(1-50).GST,V1.pcDNA3
 
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RRID:Addgene_21795 AIRAP Mus musculus Ampicillin PMID:16973439 Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin amino acids 1-50 2026-07-25 12:44:27 0
SCP1-pUC119
 
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RRID:Addgene_21846 SCP1 (super core promoter 1)-pUC119 Drosophila melanogaster Ampicillin PMID:17124735 There are several mismatches between depositor's sequence and Addgene sequence. The mismatches are mostly in vector and should not affect the insert. Backbone Size:3162; Vector Backbone:pUC119; Vector Types:pUC derivative - a cloning vector; Bacterial Resistance:Ampicillin 2026-07-25 12:44:28 0
pXDC61-FabI
 
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1+ mentions
RRID:Addgene_21842 fabI L. pneumophila Chloramphenicol PMID:19578436 Backbone Size:9869; Vector Backbone:pXDC61; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-07-25 12:44:28 1
haA1.pBABEpu
 
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1+ mentions
RRID:Addgene_21813 GADD34 C-term Cricetulus longicaudatus (long-tailed hamster) Ampicillin PMID:11381086 Hamster GADD34 C-term was isolated in a somatic cell genetic screen as a high dose supressor of the intergated stess response. The insert from the retrovirus isolated in that screen was recloned into unique BamH1 and Sal1 sites of pBABEpu and confirmed to suppress the ISR when introduced back into cells. Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-07-25 12:44:28 2
pQE-nTR
 
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RRID:Addgene_21812 pQE-nTR Homo sapiens Ampicillin PMID:15226295 Backbone Marker:Qiagen; Backbone Size:4700; Vector Backbone:pQE-80L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin His tag and aa95-113 were removed from pQE-hTR 2026-07-25 12:44:28 2
PERK 4: PERKKD-pGEX4T-1
 
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RRID:Addgene_21817 PERK (537-1114) Mus musculus Ampicillin PMID:9930704 Mouse PERK (537-1114), wild-type, 9E10-tagged at C-terminus, fused to GST, bacterial expression vector, pGEX4T-1 Sequencing found a S1109T mutation. There is no evidence that the mutation affects activity. Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin PERK Kinase Domain. Amino acids 537-1114. 2026-07-25 12:44:28 3
PERK 3: PERK.dC.9E10.pCDNA
 
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1+ mentions
RRID:Addgene_21816 PERK (C-term deleted) Mus musculus Ampicillin PMID:9930704 Mouse PERK, C-term deleted (removes amino-acids 582-1081), 9E10-tagged at C-terminus, mammalian expression vector. Sequencing found a S1109T mutation. There is no evidence that the mutation affects activity. Backbone Size:4756; Vector Backbone:pCDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin amino-acids 582-1081 deleted 2026-07-25 12:44:28 2
YIPlac204TKC-GFP-HDEL
 
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RRID:Addgene_21771 Green Fluorescent Protein (GFP) Aequorea victoria Ampicillin PMID:11285273 Backbone Size:4000; Vector Backbone:YIPlac204; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-07-25 12:44:27 0
pES1002
 
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RRID:Addgene_21789 Fus3-as2 Saccharomyces cerevisiae Ampicillin PMID:19079053 There are several sequence discrepancies between depositor's sequence and Addgene sequence in the 5' UTR. The 5'UTR in the plasmid is from a W303-derived strain, and the SGD used S288C strain for genome sequencing, so the mismatches could be SNPs. This plasmid is functional and these mismatches do not affect the ORF. Backbone Size:4700; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin contains 5' 1kb upstream UTR/promoter Q93A mutation in open reading frame 3' 400bp downstream UTR/terminator 2026-07-25 12:44:27 0
pRY1004
 
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RRID:Addgene_21788 Dig1-YFP Saccharomyces cerevisiae Ampicillin PMID:19079053 Construction was complicated; please refer to parent article's supplemental materials for details of construction. Backbone Size:3300; Vector Backbone:pACL7-YFP-A206K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin contains 5' 1000bp UTR (Dig1 promoter) Dig1 open reading frame YFP 3' ADH terminator from pACL7-YFP-A206K 2026-07-25 12:44:27 0

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