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http://www.addgene.org/30456

Species:
Genetic Insert: histone GFP
Vector Backbone Description: Backbone Marker:Addgene plasmid 12337; Backbone Size:8000; Vector Backbone:pCSC-SP-PW-GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_30456 Copy   

•   Source: Addgene

http://www.addgene.org/30698

Species: Danio rerio
Genetic Insert: F1 GCC
Vector Backbone Description: Backbone Size:6141; Vector Backbone:pCS2; Vector Types:mRNA production; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_30698 Copy   

•   Source: Addgene

http://www.addgene.org/30687

Species:
Genetic Insert: F1 AAG
Vector Backbone Description: Backbone Size:2877; Vector Backbone:pBlueScript II SK(+); Vector Types:Cloning vector; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_30687 Copy   

•   Source: Addgene

http://www.addgene.org/21835

Species: Mus musculus
Genetic Insert: GADD34
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: The construct was created by recovering the Sma1 fragment of mMyD116 and ligating it into the unique SnB1 site of pBABEpu. The stop codo is deep in the downstream SV40 promoter sequence andowing the encoded poly-peptide with a tail of nonesense sequence, but it does serve as an expressed negative control for the effects of GADD34.

Proper citation: RRID:Addgene_21835 Copy   

•   Source: Addgene

http://www.addgene.org/21834

Species: Mus musculus
Genetic Insert: GADD34
Vector Backbone Description: Backbone Size:4724; Vector Backbone:pCMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_21834 Copy   

•   Source: Addgene

http://www.addgene.org/21833

Species: Mus musculus
Genetic Insert: XBP-1 processed/spliced
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene sequencing results show two mismatches with author's sequence causing two amino acid mutations - G26V and A76V. FLAG-epitope tagged IRE1-spliced XBP-1 in the mammalian expression plasmid pFLAG-CMV-2. Flag-tagged version of XBP-1 was constructed by inserting the Flag epitope tag sequence immediately 3' of the ATG start codon. Note: Cheah Kathryn Song Eng reported on November 4, 2004 that the sequence of this plasmid deviates from the predicted by G1028T. This impacts on the CDS changing Gly27Val. We do not know if this is a mutation that crept into our construction, a mutation that was present in the cDNA we purchased or if it is a point in which the mouse genome has some variability. Nor do we know the functional significance of this, but we note that the corresponding residue in the human is Ala. Furthermore, this variation/mutation is in a region of relatively poor sequence conservation across species, suggesting that it might not be all that important.

Proper citation: RRID:Addgene_21833 Copy   

•   Source: Addgene

http://www.addgene.org/21832

Species: Mus musculus
Genetic Insert: XBP-1 unprocessed
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:4700; Vector Backbone:pFLAG-CMV2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Addgene sequencing results show two mismatches with author's sequence causing two amino acid mutations - G27V and A76V. FLAG-epitope tagged unspliced XBP-1 in the mammalian expression plasmid pFLAG-CMV-2. Flag-tagged version of XBP-1 was constructed by inserting the Flag epitope tag sequence immediately 3' of the ATG start codon. Note: Cheah Kathryn Song Eng reported on November 4, 2004 that the sequence of this plasmid deviates from the predicted by G1028T. This impacts on the CDS changing Gly27Val. We do not know if this is a mutation that crept into our construction, a mutation that was present in the cDNA we purchased or if it is a point in which the mouse genome has some variability. Nor do we know the functional significance of this, but we note that the corresponding residue in the human is Ala. Furthermore, this variation/mutation is in a region of relatively poor sequence conservation across species, suggesting that it might not be all that important.

Proper citation: RRID:Addgene_21832 Copy   

•   Source: Addgene

http://www.addgene.org/21798

Species: Mus musculus
Genetic Insert: PSMB2
Vector Backbone Description: Backbone Size:5921; Vector Backbone:pCEFL; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_21798 Copy   

•   Source: Addgene

http://www.addgene.org/21830

Species: Homo sapiens
Genetic Insert: TLS-CHOP
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2900; Vector Backbone:pBluescript KS+; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Human TLS-CHOP fusion oncogene full-length cDNA in pBS KS+ cloning vector Insert between BamH1 and Xho1. A human CHOP cDNA probe can be excised from this plasmid by Pst1-Xho1 digestion.

Proper citation: RRID:Addgene_21830 Copy   

•   Source: Addgene

http://www.addgene.org/21796

Species: Mus musculus
Genetic Insert: AIRAP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_21796 Copy   

•   Source: Addgene

http://www.addgene.org/21795

Species: Mus musculus
Genetic Insert: AIRAP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_21795 Copy   

•   Source: Addgene

http://www.addgene.org/21846

Species: Drosophila melanogaster
Genetic Insert: SCP1 (super core promoter 1)-pUC119
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC119; Vector Types:pUC derivative - a cloning vector; Bacterial Resistance:Ampicillin
References:
Comments: There are several mismatches between depositor's sequence and Addgene sequence. The mismatches are mostly in vector and should not affect the insert.

Proper citation: RRID:Addgene_21846 Copy   

•   Source: Addgene

http://www.addgene.org/21842

Species: L. pneumophila
Genetic Insert: fabI
Vector Backbone Description: Backbone Size:9869; Vector Backbone:pXDC61; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
References:
Comments:

Proper citation: RRID:Addgene_21842 Copy   

•   Source: Addgene

http://www.addgene.org/21813

Species: Cricetulus longicaudatus (long-tailed hamster)
Genetic Insert: GADD34 C-term
Vector Backbone Description: Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
References:
Comments: Hamster GADD34 C-term was isolated in a somatic cell genetic screen as a high dose supressor of the intergated stess response. The insert from the retrovirus isolated in that screen was recloned into unique BamH1 and Sal1 sites of pBABEpu and confirmed to suppress the ISR when introduced back into cells.

Proper citation: RRID:Addgene_21813 Copy   

•   Source: Addgene

http://www.addgene.org/21812

Species: Homo sapiens
Genetic Insert: pQE-nTR
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4700; Vector Backbone:pQE-80L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_21812 Copy   

•   Source: Addgene

http://www.addgene.org/21817

Species: Mus musculus
Genetic Insert: PERK (537-1114)
Vector Backbone Description: Backbone Marker:Amersham; Backbone Size:5000; Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Mouse PERK (537-1114), wild-type, 9E10-tagged at C-terminus, fused to GST, bacterial expression vector, pGEX4T-1 Sequencing found a S1109T mutation. There is no evidence that the mutation affects activity.

Proper citation: RRID:Addgene_21817 Copy   

•   Source: Addgene

http://www.addgene.org/21816

Species: Mus musculus
Genetic Insert: PERK (C-term deleted)
Vector Backbone Description: Backbone Size:4756; Vector Backbone:pCDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: Mouse PERK, C-term deleted (removes amino-acids 582-1081), 9E10-tagged at C-terminus, mammalian expression vector. Sequencing found a S1109T mutation. There is no evidence that the mutation affects activity.

Proper citation: RRID:Addgene_21816 Copy   

•   Source: Addgene

http://www.addgene.org/21771

Species: Aequorea victoria
Genetic Insert: Green Fluorescent Protein (GFP)
Vector Backbone Description: Backbone Size:4000; Vector Backbone:YIPlac204; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments:

Proper citation: RRID:Addgene_21771 Copy   

•   Source: Addgene

http://www.addgene.org/21789

Species: Saccharomyces cerevisiae
Genetic Insert: Fus3-as2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: There are several sequence discrepancies between depositor's sequence and Addgene sequence in the 5' UTR. The 5'UTR in the plasmid is from a W303-derived strain, and the SGD used S288C strain for genome sequencing, so the mismatches could be SNPs. This plasmid is functional and these mismatches do not affect the ORF.

Proper citation: RRID:Addgene_21789 Copy   

•   Source: Addgene

http://www.addgene.org/21788

Species: Saccharomyces cerevisiae
Genetic Insert: Dig1-YFP
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pACL7-YFP-A206K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
References:
Comments: Construction was complicated; please refer to parent article's supplemental materials for details of construction.

Proper citation: RRID:Addgene_21788 Copy   

•   Source: Addgene