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Vector Backbone Description: Backbone Size:5879; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Comments: Use for puromycin selection of transgenes in C. elegans and related species if transgene has a visual phenotype and Pmyo-2::mCherry expression is undesirable.
The unc-119 gene in pCG150 was replaced by the Prpl-28::PuroR::let-858_3'UTR cassette for puromycin selection in worms.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26301 Copy
Vector Backbone Description: Backbone Size:5969; Vector Backbone:pBCN21-R4R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729840
Comments: Use for G418 selection of transgenes in C. elegans.
The PuroR gene in pBCN21-R4R3 was replaced by the NeoR gene for G418 resistance.
This is a Gateway 3-fragment compatible destination vector.
It contains AttR4 and AttR3 sites (not AttR1 and AttR2 sites as identified automatically by the Addgene algorithm).
Proper citation: RRID:Addgene_26304 Copy
Species: Homo sapiens
Genetic Insert: GAL4::p65
Vector Backbone Description: Backbone Size:8778; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26229 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL4
Vector Backbone Description: Backbone Size:9052; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Comments: Addgene NGS results identified a second GAL4 ORF in the plasmid sequence. This second ORF is not expected to affect plasmid function as described in the associated publication.
Proper citation: RRID:Addgene_26226 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL80
Vector Backbone Description: Backbone Size:9409; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26236 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GAL80
Vector Backbone Description: Backbone Size:9409; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26235 Copy
Species: Homo sapiens
Genetic Insert: LexA::p65
Vector Backbone Description: Backbone Size:8747; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26231 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Zip-GAL4DBD
Vector Backbone Description: Backbone Size:8731; Vector Backbone:pBPGUw; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20697123
Proper citation: RRID:Addgene_26233 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’
Vector Backbone Description: Backbone Size:16320; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16499902
Comments: Use ONLY DB3.1 to transform this plasmid. DH5 alpha does not work (it has a death gene).
Proper citation: RRID:Addgene_22720 Copy
Species: artifical
Genetic Insert: Gateway-SnAvi
Vector Backbone Description: Backbone Marker:pTriEx5 (Novagen); Backbone Size:6027; Vector Backbone:pBY2887; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20047968
Proper citation: RRID:Addgene_23222 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:11282; Vector Backbone:pPIC3.5K; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pPIC3.5K Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction for integration of gene of interest into Pichia.
Proper citation: RRID:Addgene_18707 Copy
Vector Backbone Description: Backbone Size:9313; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_19065 Copy
Vector Backbone Description: Backbone Size:9778; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_19067 Copy
Vector Backbone Description: Backbone Size:10301; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Comments: There are several mismatches between Addgene's quality control sequence and depositor's reference sequence. The mismatches are in non-coding regions and do not affect the features.
Proper citation: RRID:Addgene_19066 Copy
Vector Backbone Description: Backbone Size:9599; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Proper citation: RRID:Addgene_19068 Copy
Species: Caenorhabditis elegans
Genetic Insert: pDESTttTi5605[R4-R3]
Vector Backbone Description: Backbone Size:2354; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18953339
Comments: Invitrogen Gateway 3-fragment compatible destination vector. Contains C.Briggsae unc-119 rescue fragment. Also contains genomic regions flanking ttTi5605 Mos1 insertion to generate MosSCI inserts at this locus.
Please note that due to a bug in the Addgene map algorithm, the attR sites are depicted as attR1 and attR2, when in fact they are attR3 and attR4. Please refer to the author's map for an accurate depiction of these sites.
Proper citation: RRID:Addgene_19329 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC91; Vector Types:Worm Expression, FLP/FRT, Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19630 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19632 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19631 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19634 Copy
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