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Species: Caenorhabditis elegans
Genetic Insert: slo-1(A2;B2;C0)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34656 Copy
Species: Caenorhabditis elegans
Genetic Insert: slo-1(A1;B2;C1)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34659 Copy
Species: Caenorhabditis elegans
Genetic Insert: slo-1(A1;B1;C1)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34658 Copy
Species: Caenorhabditis elegans
Genetic Insert: Peft-3 GFP H2B tbb-2 UTR
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Comments: We would like to thank Andrew Wood and Barbara Meyer for sending us the Peft-3 promoter and pointing out how strong the promoter is.
Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_34877 Copy
Species: Caenorhabditis elegans
Genetic Insert: tbb-2 gld-1 operon
Vector Backbone Description: Vector Backbone:pDONRP2-P3R; Vector Types:Worm targeting; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34875 Copy
Species: Caenorhabditis elegans
Genetic Insert: Mos1 transpoase
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Comments: Please note: eft-3 has officially been changed to eef-1A.1 Please see the eef-1A.1 WormBase entry for details: http://www.wormbase.org/species/c_elegans/gene/WBGene00001168#05-9g-3
Proper citation: RRID:Addgene_34874 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi5605 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34866 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Comments: Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products.
Proper citation: RRID:Addgene_34865 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Comments: Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products.
Proper citation: RRID:Addgene_34863 Copy
Species: Caenorhabditis elegans
Genetic Insert: wrmScarlet11^SEC^3xMyc
Vector Backbone Description: Backbone Size:3881; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Comments: The split-wrmScarlet system was developed and published by Goudeau et al. Genetics 2021. pGLOW63 is a derivative of published TagRFP-SEC vectors (Dickinson et al. Genetics 2015). It has a 3xMyc tag in place of 3xFlag and Lox2272 sites in place of LoxP. These features allow pGLOW63 to be used in a genetic background that has already been modified using a green FP-SEC vector, without conflicts between epitope tags and Lox sites. pGLOW63 must also be used in a genetic background that has wrmScarlet1-10. Made by Amelie Perez (Glow Worms '21).
Proper citation: RRID:Addgene_178877 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-2
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:3700; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_178898 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-2
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7439; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_178796 Copy
Species: Caenorhabditis elegans
Genetic Insert: EGL-3
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7538; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_178791 Copy
Species: Caenorhabditis elegans
Genetic Insert: UNC-9
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:8030; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22099460
Proper citation: RRID:Addgene_179224 Copy
Species: Caenorhabditis elegans
Genetic Insert: UNC-7
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7737; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22099460
Proper citation: RRID:Addgene_179220 Copy
Species: Caenorhabditis elegans
Genetic Insert: UNC-9
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:8291; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22099460
Proper citation: RRID:Addgene_179247 Copy
Species: Caenorhabditis elegans
Genetic Insert: UNC-9
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:9228; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22099460
Proper citation: RRID:Addgene_179249 Copy
Species: Caenorhabditis elegans
Genetic Insert: TWK-18
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:8031; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22099460
Proper citation: RRID:Addgene_179250 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:5769; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179561 Copy
Species: Caenorhabditis elegans
Genetic Insert: tomm20::miniSOG
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7986; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29360035
Proper citation: RRID:Addgene_179581 Copy
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