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Species: reporter gene Venus
Vector Backbone Description: Backbone Marker:Scott Barolo; Backbone Size:10474; Vector Backbone:pGreen H-Pelican; Vector Types:reporter construct; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20506180
Comments: AmpR in backbone; CmR in gateway RfA cassette
Proper citation: RRID:Addgene_24568 Copy
Species: reporter gene eGFP
Vector Backbone Description: Backbone Marker:Scott Barolo; Backbone Size:10444; Vector Backbone:pGreen H-Pelican; Vector Types:reporter construct; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20506180
Comments: AmpR in backbone; CmR in gateway RfA cassette
Proper citation: RRID:Addgene_24566 Copy
Genetic Insert: Gateway(TM) cassette
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Comments: The CMV promoter from the pLJM1 vector (Addgene) was PCR amplified and cloned into pLD-puro-EcVA using NdeI/MluI to generate pLD-puro-CcVA.
pLD-puro-EcVA (pLD-puromycin resistance- EF1alpha, C-terminal VA tag) was generated as described in the paper from pLKO.1
VA tag= versatile affinity tag, 3XFlag+2XTev+6XHis+2XStrepIII
Proper citation: RRID:Addgene_24588 Copy
Genetic Insert: None
Vector Backbone Description: Backbone Marker:Iain Fraser; Backbone Size:13170; Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16945906
Comments: Parent lentivector, co-expression of Venus selection gene
Proper citation: RRID:Addgene_25734 Copy
Vector Backbone Description: Backbone Marker:Iain Fraser; Backbone Size:12944; Vector Backbone:pSLIK; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16945906
Comments: Parent lentivector, co-expression of Zeocin selection gene
Proper citation: RRID:Addgene_25736 Copy
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:7934; Vector Backbone:pET19b; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pET19b Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal His tag. Note that the Addgene verified sequence detects a mutation in the enterokinase site D(V)DDK.
Proper citation: RRID:Addgene_18668 Copy
Genetic Insert: Gateway cassette A
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pMXs; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16904174
Comments: The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
Proper citation: RRID:Addgene_18656 Copy
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:6932; Vector Backbone:pFlag-CMV-2; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pFLAG-CMV-2 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an N-terminal FLAG tag.
Proper citation: RRID:Addgene_18700 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7755; Vector Backbone:pcDNA3.1(-)/Myc-His B; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pcDNA3.1(-)/myc-His B Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing an C-terminal myc-His tag.
Proper citation: RRID:Addgene_18701 Copy
Vector Backbone Description: Backbone Size:8718; Vector Backbone:pJG4-5; Vector Types:Yeast Expression, two-hybrid prey vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pJG4-5 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a prey vector expression clone for yeast two-hybrid.
Proper citation: RRID:Addgene_18704 Copy
Vector Backbone Description: Backbone Size:12414; Vector Backbone:pEG202; Vector Types:Yeast Expression, two-hybrid bait vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pEG202 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a bait vector expression clone for yeast two-hybrid.
Proper citation: RRID:Addgene_18705 Copy
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pRSET; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18522753
Comments: pGWF1 s a Destination Vector. It carries the lethal ccdB gene (The ccdB gene is replaced with your gene of interest during the LR reaction). The Destination Vectors will only grow on ccdB resistant cells from Invitrogen, Catalog no. A10460.
Proper citation: RRID:Addgene_18856 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19635 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19637 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R4-R3)
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19639 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)-iA-FLP::let-858(3’)-(Cbr)unc-119(+)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pGC180; Vector Types:Worm Expression, FLP/FRT; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19618 Copy
Vector Backbone Description: Backbone Size:9205; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:19657394
Comments: There are minor discrepancies between Addgene's quality control sequence and the reference sequence provided by the depositing lab. These discrepancies should not affect plasmid function.
Proper citation: RRID:Addgene_19732 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R1-R2)-L4440
Vector Backbone Description: Backbone Size:0; Vector Backbone:L4440; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19680 Copy
Species: Caenorhabditis elegans
Genetic Insert:
Defining Citation: PMID:18723890
Proper citation: RRID:Addgene_19671 Copy
Species: Caenorhabditis elegans
Genetic Insert: Gateway(R2-R1)-L4440
Vector Backbone Description: Backbone Size:0; Vector Backbone:L4440; Vector Types:RNAi, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18723890
Comments: Sequence mismatches between Addgene sequence and depositor sequence do not affect function.
Proper citation: RRID:Addgene_19679 Copy
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