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Species: E. coli
Genetic Insert: HSP33 cys
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5190; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16054089
Comments: The HSP-FRET probe was generated by inserting YFP into the pECFP-N1 plasmid between the NheI and BglII sites and then ligating the redox-sensitive regulatory domain from the Escherichia coli HSP-33 between YFP and CFP via the EcoRI and BamHI sites. HSP-33: 210 amino acids; BA000007 region 4241962-4242826. Made in Hammerling lab; used in Waypa et al., Increases in mitochondrial reactive oxygen species trigger hypoxia-induced calcium responses in pulmonary artery smooth muscle cells, Circ Res. 2006 Oct 27; 99(9):970-8 PMID: 17008601.
Proper citation: RRID:Addgene_16076 Copy
Species: E. coli
Genetic Insert: Relevant genotype: supE, thi, Δ(lac-proAB), [F' traD36, proAB, lacIqZΔM15]
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None
Proper citation: RRID:Addgene_50349 Copy
Species: E. coli
Genetic Insert: Relevant genotype: ara, Δ(lac-proAB), rspL(+strA), ϕ80, lacZΔM15
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None
Proper citation: RRID:Addgene_50348 Copy
Species: E. coli
Genetic Insert: E. coli RtcB
Vector Backbone Description: Backbone Marker:EMD / Novagen; Backbone Size:5230; Vector Backbone:pET-53-DEST; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26001965
Comments: Use expression strain: BL21 (DE3) RIPL
Proper citation: RRID:Addgene_51282 Copy
Species: E. coli
Genetic Insert: FIS
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23893733
Proper citation: RRID:Addgene_51557 Copy
Species: E. coli
Genetic Insert: FIS
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51564 Copy
Species: E. coli
Genetic Insert: ltDHFR(DD)
Vector Backbone Description: Backbone Marker:Garry Nolan ; Backbone Size:6943; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23991108
Comments: This N-terminal ltDHFR(DD) plasmid is the clone#22 in Table 1
Proper citation: RRID:Addgene_47076 Copy
Species: e. coli
Genetic Insert: lac repressor
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20576148
Proper citation: RRID:Addgene_45393 Copy
Species: E. coli
Genetic Insert: Streptavidin-Glutamate_Tag
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24056174
Proper citation: RRID:Addgene_46367 Copy
Species: E. coli
Genetic Insert: RhyB sRNA
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Proper citation: RRID:Addgene_52999 Copy
Species: E. coli
Genetic Insert: RhyB sRNA
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Proper citation: RRID:Addgene_53001 Copy
Species: E. coli
Genetic Insert: OxyS sRNA
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Proper citation: RRID:Addgene_53006 Copy
Species: E. coli
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_53032 Copy
Species: E. coli
Genetic Insert: fimE
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_53015 Copy
Species: E. coli
Genetic Insert: etpBA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBAD/myc-His A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19707189
Comments: Please note that there is a minor mutation in etpB - E64G. This mutation should not affect function.
Proper citation: RRID:Addgene_53532 Copy
Species: E. coli
Genetic Insert: etpC
Vector Backbone Description: Backbone Size:3719; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, pACYC184; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19707189
Comments: Please note that there is a L to M mutation in etpC. This mutation should not affect function.
Proper citation: RRID:Addgene_53533 Copy
Species: e. coli
Genetic Insert: pLux
Vector Backbone Description: Backbone Size:1000; Vector Backbone:pLuxR; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24104480
Proper citation: RRID:Addgene_53544 Copy
Species: E. coli
Genetic Insert: Recoded E. coli genome with all instances of the UAG codon and RF1 removed (UAG termination function removed)
Vector Backbone Description: Vector Backbone:E. coli genome; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24136966
Comments: E. coli MG1655 Delta(ybhB-bioAB)::[lcI857 N(cro-ea59)::tetR-bla] Delta prfA Delta mutS::zeoR; all 321 UAG codons changed to UAA
This strain is mutS-, which causes a spontaneous mutation frequency of ~2E-8. This strain is auxotrophic for d-biotin.
A mutS+ version of this strain will be deposited with addgene soon.
Genome sequence deposited at NCBI (accession # CP006698)
Proper citation: RRID:Addgene_48998 Copy
Species: E. coli
Genetic Insert: Recoded E. coli with all UAG codons and RF1 removed (UAG termination abolished); decreased mutation rate; 37C growth tolerated
Vector Backbone Description: Vector Backbone:E. coli genome; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:24136966
Comments: E. coli MG1655 Delta(ybhB-bioAB)::zeoR Delta prfA; all 321 UAG codons changed to UAA
This strain is mutS+, which causes a normal spontaneous mutation frequency of ~1E-10. This strain is auxotrophic for d-biotin.
The genome sequence of a similar strain (C321.deltaA) was deposited at NCBI (accession # CP006698.1).
Proper citation: RRID:Addgene_49018 Copy
Species: E. coli
Genetic Insert: HPH
Vector Backbone Description: Backbone Marker:Messing Lab; Vector Backbone:pFF19, Addgene plasmid #49777; Vector Types:plant expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1369289
Comments: polylinker: SalI, XbaI, BamHI, SmaI, KpnI, SstI/SacI, NruI
Proper citation: RRID:Addgene_49783 Copy
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