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Species: Caenorhabditis elegans
Genetic Insert: cog-1 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33159 Copy
Species: Caenorhabditis elegans
Genetic Insert: Unc104
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pET17; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12351789
Proper citation: RRID:Addgene_24476 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-119
Vector Backbone Description: Backbone Marker:Epicentre; Backbone Size:2100; Vector Backbone:pMOD4; Vector Types:Recombineering; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19116030
Comments: Few mismatches between Addgene and depositor sequence are in the 3' UTR and shouldn't have any impact on plasmid's function
Proper citation: RRID:Addgene_19185 Copy
Species: Caenorhabditis elegans
Genetic Insert: mes-3 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19371 Copy
Species: Caenorhabditis elegans
Genetic Insert: puf-5 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19372 Copy
Species: Caenorhabditis elegans
Genetic Insert: daz-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19368 Copy
Species: Caenorhabditis elegans
Genetic Insert: tbb-2 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19401 Copy
Species: Caenorhabditis elegans
Genetic Insert: msp-142 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19367 Copy
Species: Caenorhabditis elegans
Genetic Insert: spn-4 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19369 Copy
Species: Caenorhabditis elegans
Genetic Insert: pie-1 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19405 Copy
Species: Caenorhabditis elegans
Genetic Insert: par-5 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19404 Copy
Species: Caenorhabditis elegans
Genetic Insert: fbf-2 ORF+3' in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19406 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi5605 targeting region
Vector Backbone Description: Backbone Size:2334; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Standard Multiple Cloning site vector to generate MosSCI inserts at ttTi5605 location. Contains C.Briggsae unc-119 rescue gene. Also contains genomic regions flanking the ttTi5605 Mos1 insertion. Use C.elegans strain EG4322 to generate insertions.
Proper citation: RRID:Addgene_19330 Copy
Species: Caenorhabditis elegans
Genetic Insert: Phsp-16::Mos1 transposase
Vector Backbone Description: Backbone Size:2530; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: This plasmid was made by Dr. Jean Louis Bessereau and described in the publication: Bessereau JL, Wright A, Williams DC, Schuske K, Davis MW, Jorgensen EM.
Mobilization of a Drosophila transposon in the Caenorhabditis elegans germ line.
Nature. 2001 Sep 6;413(6851):70-4.
There is che-1 sequence in the plasmid. It comes from the glh-2 3' UTR fragment in the plasmid. The UTR fragment extends into the 3' region of the che-1 gene, which is tail to tail with glh-2 on the chromosome. This is only the last exon of che-1 and it is not relevant to the function of the construct.
Proper citation: RRID:Addgene_19333 Copy
Species: Caenorhabditis elegans
Genetic Insert: mes-2 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19397 Copy
Species: Caenorhabditis elegans
Genetic Insert: fbf-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19396 Copy
Species: Caenorhabditis elegans
Genetic Insert: pgl-1 3'utr in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_19399 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-3::twk-18(cn110)cDNA
Vector Backbone Description: Backbone Size:2446; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Use as co-injection marker. Negative selection marker expressed in the body wall muscles. Animals (almost) normal at 15C, paralyzed at 25C.
Proper citation: RRID:Addgene_19326 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-3::mCherry
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2416; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Red mCherry expression in the pharynx driven by the myo-3 promoter. unc-54 utr.
Proper citation: RRID:Addgene_19328 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::mCherry
Vector Backbone Description: Backbone Size:2446; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18953339
Comments: Use as co-injection marker. Red mCherry fluorescence in the pharynx. Do not use at high concentration (above 5 ng/ul) as it is toxic to animals.
Proper citation: RRID:Addgene_19327 Copy
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