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Species: Synthetic
Genetic Insert: miR-122 sponge
Vector Backbone Description: Backbone Size:5915; Vector Backbone:pAAV TBG FFluc; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388288
Comments: Please note that there is a 4bp deletion in Addgene's quality control sequence when compared to depositor's reference sequence. This deletion will not affect plasmid function.
Proper citation: RRID:Addgene_35657 Copy
Species: Synthetic
Genetic Insert: 3 miR-122 target sites
Vector Backbone Description: Vector Backbone:pAAVf-EnhCB-lacZnls; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388288
Proper citation: RRID:Addgene_35644 Copy
Species: Synthetic
Genetic Insert: TuD miR-122
Vector Backbone Description: Backbone Size:4903; Vector Backbone:pAAVscCBPIGpluc7xmiR-122BT; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388288
Proper citation: RRID:Addgene_35649 Copy
Species: Synthetic
Genetic Insert: pEBFP2-Nuc
Vector Backbone Description: Backbone Size:6782; Vector Backbone:pLenti-MP2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the Miami project.
Proper citation: RRID:Addgene_36085 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:6782; Vector Backbone:pLenti-MP2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the Miami project.
Proper citation: RRID:Addgene_36084 Copy
Species: Synthetic
Genetic Insert: 4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16720273
Comments: Genetically encoded, ratiometric, calcium biosensor that contains enhanced CFP and circularly permuted Venus 173. D3cpv does not bind wild-type calmodulin. It has a Kd' of 0.6 uM.
See also Palmer & Tsien (2006) Nature Protocols.
The small discrepancy between the Addgene and depositor's sequence should not alter the function of the plasmid.
Proper citation: RRID:Addgene_36324 Copy
Species: Synthetic
Genetic Insert: Golgi-ZapCY1 high affinity Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21502528
Comments: Second generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins.
Targeted to Golgi: N-term fusion of the first 60 amino acids of GalT
Proper citation: RRID:Addgene_36322 Copy
Species: Synthetic
Genetic Insert: ER-ZapCY1 high affinity Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21502528
Comments: Second generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins.
Targeted to ER: contains C-terminal KDEL
Proper citation: RRID:Addgene_36321 Copy
Species: Synthetic
Genetic Insert: ZapCY2 high affinity Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21502528
Comments: Second generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins.
ZapCY2 is derived from ZapCY1. The first cysteine in each zinc finger is mutated to histidine.
Proper citation: RRID:Addgene_36320 Copy
Species: Synthetic
Genetic Insert: mito-ZifCY1 genetically encoded Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19363034
Comments: First generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from mammalian Zif268. mito-ZifCY1 contains 4 tandem repeats of the first 29 aa of human cox subunit 8a (MSVLTPLLLRGLTGSARRLPVPRAKIHSL). HindIII restriction sites are at the 5' and 3' ends of the mitochondrial targeting sequence.
Proper citation: RRID:Addgene_36317 Copy
Species: Synthetic
Genetic Insert: ZifCY2 genetically encoded Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19363034
Comments: First generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from mammalian Zif268. ZifCY2 is a variant of ZifCY1. The two cysteines (amino acids 237 and 242) in the Zn(II)-binding domain of ZifCY1 are mutated to histidines in ZifCY2.
Proper citation: RRID:Addgene_36316 Copy
Species: Synthetic
Genetic Insert: ChETA-TdTomato
Vector Backbone Description: Backbone Marker:K. Deisseroth Lab; Backbone Size:5435; Vector Backbone:pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA; Vector Types:AAV, Cre/Lox, Cre-Off; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22866029
Proper citation: RRID:Addgene_37089 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:K. Deisseroth Lab; Backbone Size:5603; Vector Backbone:pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA; Vector Types:AAV, Cre/Lox, Cre-On; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22866029
Proper citation: RRID:Addgene_37084 Copy
Species: Synthetic
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:K. Deisseroth Lab; Backbone Size:5613; Vector Backbone:pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA; Vector Types:AAV, Cre/Lox, Cre-Off; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22866029
Proper citation: RRID:Addgene_37085 Copy
Species: Synthetic
Genetic Insert: NpHR3.0-EYFP
Vector Backbone Description: Backbone Marker:K. Deisseroth Lab; Backbone Size:5613; Vector Backbone:pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA; Vector Types:AAV, Cre/Lox, Cre-Off; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22866029
Proper citation: RRID:Addgene_37087 Copy
Species: Synthetic
Genetic Insert: d1eGFP
Vector Backbone Description: Backbone Marker:K. Deisseroth Lab; Backbone Size:5435; Vector Backbone:pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA; Vector Types:AAV, Cre/Lox, Cre-Off; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22866029
Proper citation: RRID:Addgene_37091 Copy
Species: Synthetic
Genetic Insert: TdTomato
Vector Backbone Description: Backbone Marker:K. Deisseroth Lab; Backbone Size:5435; Vector Backbone:pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA; Vector Types:AAV, Cre/Lox, Cre-Off; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22866029
Proper citation: RRID:Addgene_37092 Copy
Species: Synthetic
Genetic Insert: hChr2(H134R)-mCherry
Vector Backbone Description: Backbone Marker:K. Deisseroth Lab; Backbone Size:5613; Vector Backbone:pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA; Vector Types:AAV, Cre/Lox, Cre-Off; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22660328
Proper citation: RRID:Addgene_37082 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:K. Deisseroth Lab; Backbone Size:5603; Vector Backbone:pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA; Vector Types:AAV, Cre/Lox, Cre-On; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22866029
Proper citation: RRID:Addgene_37083 Copy
Species: Synthetic
Genetic Insert: TdTomato-EGFP
Vector Backbone Description: Backbone Marker:K. Deisseroth Lab; Backbone Size:5627; Vector Backbone:pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA; Vector Types:AAV, Cre/Lox, Cre-Switch; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22866029
Proper citation: RRID:Addgene_37120 Copy
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