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Species: Caenorhabditis elegans
Genetic Insert: ttTi5605
Vector Backbone Description: Backbone Marker:John Calarco's lab at University of Toronto; Vector Backbone:loxP_myo2_neoR; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36640369
Proper citation: RRID:Addgene_194056 Copy
Species: Caenorhabditis elegans
Genetic Insert: ChrII_ttTi5605 sgRNA
Vector Backbone Description: Vector Backbone:pTK73; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36640369
Comments: An identical plasmid has been reported by Dr. Shinsuke Niwa's group.
doi.org/10.1371/journal.pone.0197128
Proper citation: RRID:Addgene_194058 Copy
Species: Caenorhabditis elegans
Genetic Insert: lim-4
Vector Backbone Description: Vector Backbone:pPD95.62; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378642
Proper citation: RRID:Addgene_191028 Copy
Species: Caenorhabditis elegans
Genetic Insert: Tetanus Toxin Light Chain
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7038; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36182701
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
Proper citation: RRID:Addgene_192107 Copy
Species: Caenorhabditis elegans
Genetic Insert: GtACR2
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:10495; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36182701
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
Proper citation: RRID:Addgene_191533 Copy
Species: Caenorhabditis elegans
Genetic Insert: Chrimson
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:10497; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36182701
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
Proper citation: RRID:Addgene_191539 Copy
Species: Caenorhabditis elegans
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:4992; Vector Backbone:pBSK; Vector Types:Worm Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36182701
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
Proper citation: RRID:Addgene_191538 Copy
Species: Caenorhabditis elegans
Genetic Insert: Tetanus Toxin Light Chain
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:8530; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36182701
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
Proper citation: RRID:Addgene_192061 Copy
Species: Caenorhabditis elegans
Genetic Insert: Cre
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:6679; Vector Backbone:pBSK; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36182701
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 for bioRxiv preprint.
Proper citation: RRID:Addgene_191531 Copy
Species: Caenorhabditis elegans
Genetic Insert: GCaMP3
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:5868; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36182701
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 for bioRxiv preprint.
Proper citation: RRID:Addgene_191373 Copy
Species: Caenorhabditis elegans
Genetic Insert: Chrimson
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7472; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36182701
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 for bioRxiv preprint.
Proper citation: RRID:Addgene_191479 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 region
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pDESTR4-R3; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Unpublished MosSCI reagents from the Jorgensen lab.
This vector targets the site ttTi4348 on Chr. I.
Use with strain EG6701 at the CGC.
Note - plasmid contains TWO M13F primer sites.
Proper citation: RRID:Addgene_30538 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 region
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pDESTR4-R3 derivative; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_30539 Copy
Species: Caenorhabditis elegans
Genetic Insert: Thr412Gly-CePheRS Alpha Subunit::C. elegans CEOP5428 Intercistronic Region::HA-GFP [From pPD95.75 (+118 to +1000)] in pDONR221
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2544; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: Note: the first methionine in the alpha subunit of C. elegans PheRS, Isoform A, Exon 3 is designated as residue 1 (“Met1”).
Proper citation: RRID:Addgene_72673 Copy
Species: Caenorhabditis elegans
Genetic Insert: let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2639; Vector Backbone:pDONR P2R-P3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_72674 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTurquoise2-C1^SEC^3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37351566
Comments: pDD315 is a derivative of our published vectors pDD282-285 (Dickinson et al. Genetics 2015). It has a 2xHA tag in place of 3xFlag, and Lox511I sites in place of LoxP. These features allow pDD315 to be used in a genetic background that has already been modified using a green or red FP-SEC vector, without conflicts between epitope tags and Lox sites.
Proper citation: RRID:Addgene_73343 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::GFP + SEC
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34903234
Comments: pDD317 is a derivative of our published vectors pDD282-285 (Dickinson et al. Genetics 2015) and is intended for making gene knockouts. It has a Pmyo-2::GFP expression cassette (bright pharyngeal fluorescence) in place of the simple fluorescent protein found in our other FP–SEC vectors. Pmyo-2::GFP + SEC is intended to be inserted in place of an entire ORF such that after insertion and SEC removal, the knockout is marked with Pmyo-2::GFP. The vector also has Lox511I sites in place of LoxP, allowing pDD315 to be used in a genetic background that has already been modified using a green or red FP-SEC vector, without conflicts between Lox sites.
Proper citation: RRID:Addgene_73344 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-70::TSMod
Vector Backbone Description: Vector Backbone:pEXPR; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24561618
Proper citation: RRID:Addgene_73367 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5::mKate2::PH::tbb-2
Vector Backbone Description: Vector Backbone:modified pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27385332
Proper citation: RRID:Addgene_73512 Copy
Species: Caenorhabditis elegans
Genetic Insert: pmex-5::mCherryo::PH::tbb-2
Vector Backbone Description: Vector Backbone:modified pCFJ150; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27385332
Proper citation: RRID:Addgene_73511 Copy
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