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Species: Synthetic
Genetic Insert: enCas9(H840A)-PolI5M
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40253348
Proper citation: RRID:Addgene_249064 Copy
Species: Synthetic
Genetic Insert: enCas9
Vector Backbone Description: Vector Backbone:n/a; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40253348
Proper citation: RRID:Addgene_249063 Copy
Species: Synthetic
Genetic Insert: mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)
Vector Backbone Description: Vector Backbone:pCAGEN-DV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_242591 Copy
Species: Synthetic
Genetic Insert: CLOMELEON (CFP-YFP fusion with ENLYFQG-linker)
Vector Backbone Description: Backbone Marker:Andrew Gleave; Backbone Size:5042; Vector Backbone:pART7; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15086798
Comments: pART7::CLOMELEON is a pART7 vector with the CLOMELEON gene (Kuner, T. & Augustine, G.J.; PubMed: 11055428; doi: 10.1016/S0896-6273(00)00056-8) integrated between EcoRI and HindIII restriction sites. See the Supplemental Document under Resource Information for more details, including annotated maps.
Proper citation: RRID:Addgene_247987 Copy
Species: Synthetic
Genetic Insert: 3'UTR cloning site
Vector Backbone Description: Backbone Marker:Addgene #84771; Vector Backbone:BTV; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39014073
Proper citation: RRID:Addgene_213963 Copy
Species: Synthetic
Genetic Insert: NLS-NOS terminator-Hygromycin resistance
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pGGF000; Vector Types:GreenGate cloning entry vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_246819 Copy
Species: Synthetic
Genetic Insert: ZmUBI Promoter-scFV-sfGFP
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pGGD000; Vector Types:GreenGate cloning entry vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_246802 Copy
Species: Synthetic
Genetic Insert: smGFP-HA
Vector Backbone Description: Vector Backbone:pFlpStop-attB-UAS-2.1-tdTom; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41310158
Proper citation: RRID:Addgene_247945 Copy
Species: Synthetic
Genetic Insert: smGFP-HA
Vector Backbone Description: Vector Backbone:pFlpStop-attB-UAS-2.1-tdTom; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41310158
Proper citation: RRID:Addgene_247946 Copy
Species: Synthetic
Genetic Insert: smGFP-HA
Vector Backbone Description: Vector Backbone:pFlpStop-attB-UAS-2.1-tdTom; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41310158
Proper citation: RRID:Addgene_247948 Copy
Species: Synthetic
Genetic Insert: GFSTF
Vector Backbone Description: Vector Backbone:pFlpStop-attB-UAS-2.1-tdTom; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41310158
Proper citation: RRID:Addgene_247949 Copy
Species: Synthetic
Genetic Insert: GFSTF
Vector Backbone Description: Vector Backbone:pFlpStop-attB-UAS-2.1-tdTom; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41310158
Proper citation: RRID:Addgene_247951 Copy
Species: Synthetic
Genetic Insert: tagBFP
Vector Backbone Description: Backbone Size:2294; Vector Backbone:pUC19; Vector Types:Mammalian Expression, Synthetic Biology, in vitro transcription; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41083901
Comments: PCR template for generating linear template for in vitro transcription. 5' and 3' beta globin UTR sequences were based on previous literature for effective modRNA expression in hiPSCs: 10.1016/j.crmeth.2022.100290The primers used for generating PCR template also came from this work, and are as follows:FWD: 5'- AGCTATAATACGACTCACTATAAGctcctgggcaacgtgctg-3', which encodes the T7 promoter compatible with AG CleanCap reagent (upper-case bases) and binds to the 5' UTR (lower-case bases)REV: 5'-poly(T)116-GCAATGAAAATAAATGTTTTTTATTAGGCAGAAT-3'}, which encodes the poly(A) tail and binds to the 3'-UTR.
Please visit https://doi.org/10.1101/2024.06.19.599813 for bioRxiv preprint.
Proper citation: RRID:Addgene_246370 Copy
Species: Synthetic
Genetic Insert: CytoTape-V5
Vector Backbone Description: Backbone Marker:Epoch Life Science, Inc.; Backbone Size:5889; Vector Backbone:pAAV-F-RAM; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41588170
Comments: Please visit https://doi.org/10.1101/2025.05.10.653182 for bioRxiv preprint.
Proper citation: RRID:Addgene_239427 Copy
Species: Synthetic
Genetic Insert: anti-GFP nanobody VHH
Vector Backbone Description: Backbone Marker:Merck - Novagen; Vector Backbone:pET24a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41182966
Proper citation: RRID:Addgene_243767 Copy
Species: Synthetic
Genetic Insert: NanoLuc
Vector Backbone Description: Vector Backbone:pCDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41335466
Proper citation: RRID:Addgene_238947 Copy
Species: Synthetic
Genetic Insert: N-Terminal tag V5
Vector Backbone Description: Backbone Size:2246; Vector Backbone:pICSL01002; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: Level 0 N-Terminal tag module used for GoldenGate assemblies using the MoClo overhang syntax
Proper citation: RRID:Addgene_245535 Copy
Species: Synthetic
Genetic Insert: C-Terminal tag 3xGly4Ser-GAL4
Vector Backbone Description: Backbone Size:2244; Vector Backbone:pICSL01003; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Comments: Level 0 C-Terminal tag module used for GoldenGate assemblies using the MoClo overhang syntax
Proper citation: RRID:Addgene_245620 Copy
Species: Synthetic
Genetic Insert: 2x Lyn Kinase membrane localization sequence
Vector Backbone Description: Backbone Marker:Twist; Backbone Size:3948; Vector Backbone:pTwist Amp High Copy; Vector Types:zebrafish mRNA expression using SP6; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41426965
Comments: see https://www.fpbase.org/protein/mgold2t/
Proper citation: RRID:Addgene_247638 Copy
Species: Synthetic
Genetic Insert: PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-PBT1318-TetR-rrnBT2-pTetR56-RBS1PLP-mGL-rrnBT2
Vector Backbone Description: Vector Backbone:pNBU2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41610848
Proper citation: RRID:Addgene_248729 Copy
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