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Species: Caenorhabditis elegans
Genetic Insert: eft-3p
Vector Backbone Description: Backbone Size:2855; Vector Backbone:pBSK; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40260543
Comments: The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
Proper citation: RRID:Addgene_245121 Copy
Species: Caenorhabditis elegans
Genetic Insert: eft-3p
Vector Backbone Description: Backbone Size:2862; Vector Backbone:pBSK; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40260543
Proper citation: RRID:Addgene_245120 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP::mlcd-1
Vector Backbone Description: Backbone Size:3616; Vector Backbone:pRSET B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41773048
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
There is a single nt discrepancy in Pgpc-1. This does not affect the plasmid's function.
Proper citation: RRID:Addgene_253863 Copy
Species: Caenorhabditis elegans
Genetic Insert: GFP::C54F6.5 3'UTR
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41773048
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
There is a single nt discrepancy in Pgpc-1. This does not affect the plasmid's function.
The EGFP in the plasmid has synthetic introns in it to help expression in C. elegans. The mutations or indels are all in the introns and does not affect the protein expression or function. We observed good GFP expression from the plasmid
Proper citation: RRID:Addgene_253862 Copy
Species: Caenorhabditis elegans
Genetic Insert: SL2 NLS mNeptune
Vector Backbone Description: Backbone Size:5900; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41773048
Comments: Please visit for https://www.biorxiv.org/content/10.1101/2021.09.21.461278v4 bioRxiv preprint.
There is a single nt discrepancy in Pgpc-1. This does not affect the plasmid's function.
The Addgene sequence result finds a 1bp indel at the 3' end of the ORF containing mCardinal that causes a premature stop codon. The indel does not affect the expression and localization of the NLS::mNeptune. The protein in the plasmid expresses a few amino acids past the final Lysine of mNeptune and therefore, the premature stop does not truncate any functional part of the fluorescent protein. We observed bright nuclear RFP signal with this plasmid.
Proper citation: RRID:Addgene_253948 Copy
Species: Caenorhabditis elegans
Genetic Insert: rap-1::mGFP
Vector Backbone Description: Vector Backbone:pCR8/GW/TOPO; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40748210
Proper citation: RRID:Addgene_245765 Copy
Species: Caenorhabditis elegans
Genetic Insert: rap-1::mCherry
Vector Backbone Description: Vector Backbone:pCR8/GW/TOPO; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40748210
Proper citation: RRID:Addgene_245764 Copy
Species: Caenorhabditis elegans
Genetic Insert: tiam-1::mGFP
Vector Backbone Description: Vector Backbone:pDEST; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40748210
Proper citation: RRID:Addgene_245769 Copy
Species: Caenorhabditis elegans
Genetic Insert: rap-1::mGFP
Vector Backbone Description: Vector Backbone:pDEST; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40748210
Proper citation: RRID:Addgene_245768 Copy
Species: Caenorhabditis elegans
Genetic Insert: rap-1(G12V)::mGFP
Vector Backbone Description: Vector Backbone:pCR8/GW/TOPO; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40748210
Proper citation: RRID:Addgene_245772 Copy
Species: Caenorhabditis elegans
Genetic Insert: rac-2::mGFP
Vector Backbone Description: Vector Backbone:pDEST; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40748210
Proper citation: RRID:Addgene_245776 Copy
Species: Caenorhabditis elegans
Genetic Insert: rac-2::mGFP
Vector Backbone Description: Vector Backbone:pCR8/GW/TOPO; Vector Types:Unspecified; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:40748210
Proper citation: RRID:Addgene_245773 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-12
Vector Backbone Description: Backbone Size:3791; Vector Backbone:pCMV myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21814518
Proper citation: RRID:Addgene_32566 Copy
Species: Caenorhabditis elegans
Genetic Insert: T04C9.2 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33199 Copy
Species: Caenorhabditis elegans
Genetic Insert: T20G5.9 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33192 Copy
Species: Caenorhabditis elegans
Genetic Insert: T20G5.9 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33191 Copy
Species: Caenorhabditis elegans
Genetic Insert: acl-5 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33196 Copy
Species: Caenorhabditis elegans
Genetic Insert: acl-5 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33194 Copy
Species: Caenorhabditis elegans
Genetic Insert: cog-1 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33222 Copy
Species: Caenorhabditis elegans
Genetic Insert: cog-1 3'UTR
Vector Backbone Description: Backbone Marker:Addgene (#12179); Backbone Size:4085; Vector Backbone:pIS1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21909094
Proper citation: RRID:Addgene_33223 Copy
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