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Species: Homo sapiens
Genetic Insert: Dynactin 2
Vector Backbone Description: Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22327364
Comments: Please note that Addgene's sequencing results identified single nucleotide mismatches at bp# 2273 & 2347 when compared to the full plasmid sequence provided by the depositing laboratory. The mismatch at bp#2273 causes A7P mutation in the DCTN2 seqeuence.
Proper citation: RRID:Addgene_37388 Copy
Species: Homo sapiens
Genetic Insert: dynein light chain TcTex-type 3
Vector Backbone Description: Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22327364
Proper citation: RRID:Addgene_37390 Copy
Species: Homo sapiens
Genetic Insert: ACTR1A
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22327364
Proper citation: RRID:Addgene_37391 Copy
Species: Homo sapiens
Genetic Insert: HsPAN3
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4759; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21981923
Proper citation: RRID:Addgene_37374 Copy
Species: Drosophila melanogaster
Genetic Insert: porcupine
Vector Backbone Description: Backbone Size:7855; Vector Backbone:pCaSpeR-4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8985181
Comments: Perrimon Lab plasmid #123
The EcoRI-XbaI genomic DNA covering the porc locus was cloned in pCaspeR4 to generate a genomic porc rescue construct.
Proper citation: RRID:Addgene_37376 Copy
Species: Drosophila melanogaster
Genetic Insert: porcupine
Vector Backbone Description: Vector Backbone:pNB40; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8985181
Comments: Perrimon Lab plasmid #124
Proper citation: RRID:Addgene_37377 Copy
Species: Homo sapiens
Genetic Insert: HsNot1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4755; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21981923
Proper citation: RRID:Addgene_37370 Copy
Species: Homo sapiens
Genetic Insert: HsPAN2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4775; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21981923
Proper citation: RRID:Addgene_37373 Copy
Species: Drosophila melanogaster
Genetic Insert: PolIII-Renilla control reporter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3320; Vector Backbone:pRL-null; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16311596
Comments: Perrimon Lab plasmid #414
The PolIII–Renilla luciferase control construct was generated by PCR amplification of a fragment (base pairs 43,224–43,389 of scaffold AE003823) of the promoter of the D. melanogaster RNA PolIII 128 subunit (RpIII128) with a 5' BglII and a 3' SpeI site and ligation of this fragment into the BglII and SpeI sites of pRL-null.
Proper citation: RRID:Addgene_37380 Copy
Genetic Insert: Cre Shine
Vector Backbone Description: Backbone Marker:Invitrogene; Backbone Size:5060; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_37404 Copy
Species: Homo sapiens
Genetic Insert: Plk1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22327364
Proper citation: RRID:Addgene_37406 Copy
Species: Homo sapiens
Genetic Insert: Plk1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22327364
Proper citation: RRID:Addgene_37407 Copy
Species: Homo sapiens
Genetic Insert: HsNot8
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4780; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21981923
Proper citation: RRID:Addgene_37367 Copy
Species: Drosophila melanogaster
Genetic Insert: tout-velu
Vector Backbone Description: Vector Backbone:pNB40; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9665133
Comments: Perrimon Lab plasmid #179
Proper citation: RRID:Addgene_37401 Copy
Species: Homo sapiens
Genetic Insert: EEF1D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21936567
Proper citation: RRID:Addgene_37364 Copy
Species: Homo sapiens
Genetic Insert: npc1l1
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5000; Vector Backbone:pFASTBAC; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21525977
Comments: There is a TEV cleavage site downstream of His tag.
Proper citation: RRID:Addgene_37366 Copy
Species: Homo sapiens
Genetic Insert: CK2beta 6KR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5542; Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17681943
Proper citation: RRID:Addgene_37361 Copy
Species: Homo sapiens
Genetic Insert: CK2α V66A/I174A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5542; Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18258654
Comments: there is a 3bp deletion (AGG) in the MCS 131.
Proper citation: RRID:Addgene_37362 Copy
Species: Synthetic
Genetic Insert: NLS-mCherry
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6000; Vector Backbone:pQC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397594
Proper citation: RRID:Addgene_37354 Copy
Species: Synthetic
Genetic Insert: membrane TdTomato
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6000; Vector Backbone:pQC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397594
Comments: *palmitoylation sequence: 5'-atgctgtgctgtatgagaagaaccaaacaggttgaaaagaatgatgaggaccaaaagatc-3'
Proper citation: RRID:Addgene_37351 Copy
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