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Species: Synthetic
Genetic Insert: de-catalyzed SaCas9
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113686 Copy
Species: Synthetic
Genetic Insert: inverted de-catalyzed SaCas9
Vector Backbone Description: Backbone Marker:Alligent Technologies; Backbone Size:6739; Vector Backbone:AAV; Vector Types:AAV, Cre/Lox, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113685 Copy
Species: Synthetic
Genetic Insert: mTagBFP2
Vector Backbone Description: Backbone Size:7894; Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34568302
Proper citation: RRID:Addgene_113725 Copy
Species: Synthetic
Genetic Insert: TagBFP
Vector Backbone Description: Backbone Size:7895; Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113724 Copy
Species: Synthetic
Genetic Insert: miRFP670
Vector Backbone Description: Backbone Size:7909; Vector Backbone:pLenti6.2; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_113726 Copy
Species: Synthetic
Genetic Insert: ncpGCaMP6s
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29934970
Proper citation: RRID:Addgene_113674 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA Cassete
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Comments: The H1TO promoter renders the gRNA inducible via the presence of Doxycycline. See: The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing. de Solis CA, Ho A, Holehonnur R, Ploski JE. Front Mol Neurosci. 2016 Aug 18;9:70. doi: 10.3389/fnmol.2016.00070. eCollection 2016. 10.3389/fnmol.2016.00070 PubMed 27587996
Proper citation: RRID:Addgene_113701 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA Cassete
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Comments: The H1TO promoter renders the gRNA inducible via the presence of Doxycycline. See: The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing. de Solis CA, Ho A, Holehonnur R, Ploski JE. Front Mol Neurosci. 2016 Aug 18;9:70. doi: 10.3389/fnmol.2016.00070. eCollection 2016. 10.3389/fnmol.2016.00070 PubMed 27587996
Proper citation: RRID:Addgene_113700 Copy
Species: Synthetic
Genetic Insert: stop cassette
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2540; Vector Backbone:pDONR221-P4rP3r; Vector Types:CRISPR, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31523744
Comments: Second position vector for three-way Gateway LR reaction to generate a premature stop codon at your locus of interest. Also contains a multiple cloning site for easy genotyping. This plasmid contains multiple M13F and M13R sites, due to cloning the MCS of pBluescriptSK+.
QC note: This plasmid dimerizes during growth in bacteria, which can result in digest and Sanger sequencing results that differ from the full plasmid sequence of the monomer. The depositing lab has confirmed that dimerization does not affect plasmid function.
Proper citation: RRID:Addgene_113745 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA Cassete
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113695 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA Cassete
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113694 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA Cassete
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113693 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA Cassete
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Comments: The U6TO promoter renders the gRNA inducible via the presence of Doxycycline. See: The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing. de Solis CA, Ho A, Holehonnur R, Ploski JE. Front Mol Neurosci. 2016 Aug 18;9:70. doi: 10.3389/fnmol.2016.00070. eCollection 2016. 10.3389/fnmol.2016.00070 PubMed 27587996
Proper citation: RRID:Addgene_113698 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA Cassete
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113697 Copy
Species: Synthetic
Genetic Insert: SaCas9 gRNA Cassete
Vector Backbone Description: Backbone Marker:Alligent Technologies; Vector Backbone:AAV; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30483052
Proper citation: RRID:Addgene_113696 Copy
Species: Synthetic
Genetic Insert: osTIR1-GFP
Vector Backbone Description: Vector Backbone:pbluescript; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29889212
Comments: This vector is good for targeting experiment in early embryos and mouse cell lines. Viability problem have been observed when generating knock-in mice.
Proper citation: RRID:Addgene_113832 Copy
Species: Synthetic
Genetic Insert: jREX-GECO1
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:4064; Vector Backbone:pTorPE; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31054773
Comments: Please visit https://www.biorxiv.org/content/early/2018/10/04/435891 for bioRxiv preprint.
Proper citation: RRID:Addgene_113941 Copy
Species: Synthetic
Genetic Insert: R-GECO1
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:4067; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31054773
Comments: Please visit https://www.biorxiv.org/content/early/2018/10/04/435891 for bioRxiv preprint.
Proper citation: RRID:Addgene_113943 Copy
Species: Synthetic
Genetic Insert: H-Luc_mito
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28941028
Proper citation: RRID:Addgene_113930 Copy
Species: Synthetic
Genetic Insert: LOV2 and Stim1 polybasic domain (PB)
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28959426
Proper citation: RRID:Addgene_113934 Copy
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