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Species: Other
Genetic Insert: 6His-TEV-TBP6.7(Q54A)
Vector Backbone Description: Backbone Size:5277; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29961805
Proper citation: RRID:Addgene_112730 Copy
Species: Mus musculus
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Feng Zhang; Backbone Size:4574; Vector Backbone:PX552; Vector Types:Mammalian Expression, AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29706865
Comments: gRNA targeting mouse Gsk3b gene was cloned into the plasmid PX552 received from Dr. Feng Zhang.
Proper citation: RRID:Addgene_112733 Copy
Species: E. coli
Genetic Insert: 2(KW)2-mCherry
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5706; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26264666
Comments: Note: The mCherry insert contains a N9Q mutation. The depositor has confirmed this does not affect plasmid function.
Proper citation: RRID:Addgene_112738 Copy
Species: E. coli
Genetic Insert: 2HMG-eGFP
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5706; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_112737 Copy
Species: Other
Genetic Insert: 6His-TEV-TBP6.7(WT)
Vector Backbone Description: Backbone Size:5277; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29961805
Proper citation: RRID:Addgene_112720 Copy
Species: Other
Genetic Insert: 6His-TEV-TBP6.7(R47A)
Vector Backbone Description: Backbone Size:5277; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29961805
Proper citation: RRID:Addgene_112722 Copy
Species: Other
Genetic Insert: 6His-TEV-TBP6.7(R49A)
Vector Backbone Description: Backbone Size:5277; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29961805
Proper citation: RRID:Addgene_112726 Copy
Species: Synthetic
Genetic Insert: EfaCas1
Vector Backbone Description: Backbone Size:5800; Vector Backbone:His6-SUMO-pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28869593
Proper citation: RRID:Addgene_112793 Copy
Species: Synthetic
Genetic Insert: Pveg-eGFP
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:3185; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Please visit https://www.biorxiv.org/content/early/2017/09/06/185108 for bioRxiv preprint.
Proper citation: RRID:Addgene_112792 Copy
Species: Synthetic
Genetic Insert: cat
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2532; Vector Backbone:pSB1A2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/early/2017/09/06/185108 for bioRxiv preprint.
Proper citation: RRID:Addgene_112791 Copy
Species: Synthetic
Genetic Insert: yckABD
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2532; Vector Backbone:pSB1A2 ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/early/2017/09/06/185108 for bioRxiv preprint.
Proper citation: RRID:Addgene_112790 Copy
Vector Backbone Description: Backbone Size:15615; Vector Backbone:pTC223; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Comments: Addgene NGS results identified an IS5 element immediately upstream of KanR, which should not affect plasmid function.
Proper citation: RRID:Addgene_112797 Copy
Species: Aequorea victoria
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5078; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30166341
Comments: The N-terminal tag sequence of this insert is exactly the same as in pcDNA4/TO-bio-myc-cCE, thus providing a specificity control for pulldown assays.
Proper citation: RRID:Addgene_112712 Copy
Species: Mus musculus
Genetic Insert: Rngtt
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5078; Vector Backbone:pcDNA4/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30166341
Comments: For more information about this plasmid, see Mukherjee et al. 2014 PLoS Biol (10.1371/journal.pbio.1001933). The insert sequence of this plasmid was subcloned from pcDNA3-bio-myc-NES-mCEΔNLS described in this publication.
Additional internal sequencing primers:
IntCE1-F: GAATGCCCCACCACTGAGAATACTG
IntCE2-F: GTTAGGAGAGGTGCAGCAGAAATGTC
IntCE3-F: CAAAGAAGTCAGCCATGAAATGGATGGAC
Proper citation: RRID:Addgene_112711 Copy
Species: Rattus norvegicus
Genetic Insert: OCTN2/SLC22A5
Vector Backbone Description: Backbone Marker:Sigma; Vector Backbone:p3 FLAG_CMV14; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24349196
Proper citation: RRID:Addgene_112799 Copy
Species: Homo sapiens
Genetic Insert: RNGTT
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5368; Vector Backbone:pET28b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28981715
Proper citation: RRID:Addgene_112710 Copy
Species: Homo sapiens
Genetic Insert: CFP-KRAS4B
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:5446; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29336889
Proper citation: RRID:Addgene_112717 Copy
Species: Mus musculus
Genetic Insert: Rngtt
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30166341
Comments: This plasmid encodes the guanylyltransferase domain of RNGTT.
Proper citation: RRID:Addgene_112716 Copy
Species: Mus musculus
Genetic Insert: Rngtt
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30166341
Comments: This plasmid encodes the triphosphatase domain of RNGTT.
Proper citation: RRID:Addgene_112715 Copy
Species: Homo sapiens
Genetic Insert: YFP-KRAS4B
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29336889
Proper citation: RRID:Addgene_112718 Copy
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