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Species: Rattus norvegicus
Genetic Insert: MAPK1
Vector Backbone Description: Backbone Marker:David Russell, UTSW; Backbone Size:4700; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11591711
Proper citation: RRID:Addgene_40820 Copy
Species: Rattus norvegicus
Genetic Insert: Erk2
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24704509
Proper citation: RRID:Addgene_40777 Copy
Species: Rattus norvegicus
Genetic Insert: syntaxin 6
Vector Backbone Description: Backbone Marker:Sigma Aldrich; Backbone Size:4717; Vector Backbone:p3XFLAG-CMV 7.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21109560
Proper citation: RRID:Addgene_50012 Copy
Species: Rattus norvegicus
Genetic Insert: Cas
Vector Backbone Description: Backbone Marker:Yamada Lab; Vector Backbone:pGZ21dxZ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_50729 Copy
Species: Rattus norvegicus
Genetic Insert: mRuby2-P2A-GCaMP6f
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27284193
Comments: The discrepancies between the full sequence and Addgene's QC sequence should not have any functional consequence.
Proper citation: RRID:Addgene_50943 Copy
Species: Rattus norvegicus
Genetic Insert: mRuby2-P2A-GCaMP6s
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27284193
Comments: The discrepancies between the full sequence and Addgene's QC sequence should not have any functional consequence.
Proper citation: RRID:Addgene_50942 Copy
Species: Rattus norvegicus
Genetic Insert: Synaptophysin-miniSOG-T2A-mCherry
Vector Backbone Description: Backbone Size:4575; Vector Backbone:AAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23889931
Comments: WPRE is inserted after the mCherry before SV40 polyA.
Proper citation: RRID:Addgene_50972 Copy
Species: Rattus norvegicus
Genetic Insert: Synaptophysin-miniSOG-citrine
Vector Backbone Description: Backbone Size:4575; Vector Backbone:AAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23889931
Comments: WPRE is inserted after the citrine before SV40 polyA.
Proper citation: RRID:Addgene_50971 Copy
Species: Rattus norvegicus
Genetic Insert: Rem2
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21485012
Comments: Please note that the sequence of the myc epitope tag in this plasmid is MQKLISEEDLL, which differs from the standard EQKLISEEDLL.
Proper citation: RRID:Addgene_51596 Copy
Species: Rattus norvegicus
Genetic Insert: shRNA2
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSuper; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24036351
Proper citation: RRID:Addgene_51598 Copy
Species: Rattus norvegicus
Genetic Insert: PKA regulatory subunit RII beta tagged by mEGFP
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19447092
Proper citation: RRID:Addgene_45523 Copy
Species: Rattus norvegicus
Genetic Insert: PKA regulatory subunit RII beta
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19447092
Proper citation: RRID:Addgene_45522 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11060023
Proper citation: RRID:Addgene_45449 Copy
Species: Rattus norvegicus
Genetic Insert: microtubule-associated protein 1 light chain 3 beta
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11060023
Proper citation: RRID:Addgene_45448 Copy
Species: Rattus norvegicus
Genetic Insert: NR2B
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4006; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12160751
Comments: Addgene Next-generation sequencing identified a sequence discrepancy that results in a Met to Leu change at position 745 in the plasmid insert, relative to Genbank IDs NM_012574.1 and NP_036706.1. The plasmid is expected to function as described in the associated publication.
Proper citation: RRID:Addgene_45447 Copy
Species: Rattus norvegicus
Genetic Insert: rM3D (Gs)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5026; Vector Backbone:pcDNA5/FRT; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17360345
Comments: These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
Proper citation: RRID:Addgene_45549 Copy
Species: Rattus norvegicus
Genetic Insert: PKA regulatory subunit RII beta tagged by mEGFP
Vector Backbone Description: Backbone Marker:Karel Svoboda; Backbone Size:5900; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, with chicken beta actin promoter and WPRE; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19447092
Proper citation: RRID:Addgene_45530 Copy
Species: Rattus norvegicus
Genetic Insert: AT1AR-324delta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15355986
Comments: Mutant AT1A receptors were generated by mutagenesis PCR using pcDNA3.1-HA-AT1A receptor (provided by M. G. Caron, Duke University) as a template and a QuikChange multisite-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions. The primer for the 324delta receptor was:
5' p-CCCCCAAAGGCCTAGCTCCCACTCAAGCC 3'.
N4D mutation introduced to prevent glycosylation at this consensus site and steric hindrance of antibody binding.
Proper citation: RRID:Addgene_45635 Copy
Species: Rattus norvegicus
Genetic Insert: TRPV4
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20650893
Proper citation: RRID:Addgene_45751 Copy
Species: Rattus norvegicus
Genetic Insert: Zc3hav1 zinc finger CCCH type, antiviral 1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18225958
Proper citation: RRID:Addgene_45908 Copy
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