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http://www.addgene.org/37184

Vector Backbone Description: Backbone Marker:CLONTECH; Backbone Size:4810; Vector Backbone:pDIRECT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24747757
Comments: For more information on Pelczar TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#pelczar

Proper citation: RRID:Addgene_37184 Copy   

•   Source: Addgene

http://www.addgene.org/37190

Species: Homo sapiens
Genetic Insert: EDD
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4708; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:12011095

Proper citation: RRID:Addgene_37190 Copy   

•   Source: Addgene

http://www.addgene.org/37192

Species: Homo sapiens
Genetic Insert: EDD C2768A
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4225; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12011095
Comments: The depositing lab has noted that mutation K503R shouldn’t be a problem as it is outside the main functional domains. There does appear to be a relatively common SNP at K503 in Latino populations so this might be a genuine SNP- http://exac.broadinstitute.org/variant/8-103338864-C-T https://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=rs571861064

Proper citation: RRID:Addgene_37192 Copy   

•   Source: Addgene

http://www.addgene.org/37217

Species: Saccharomyces cerevisiae
Genetic Insert: eIF1
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:7474; Vector Backbone:pTYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17913637

Proper citation: RRID:Addgene_37217 Copy   

•   Source: Addgene

http://www.addgene.org/37219

Species: Saccharomyces cerevisiae
Genetic Insert: eIF5
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:7474; Vector Backbone:pTYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17913637

Proper citation: RRID:Addgene_37219 Copy   

•   Source: Addgene

http://www.addgene.org/37215

Species: Mus musculus
Genetic Insert: mouse ZO-1 shRNA
Vector Backbone Description: Backbone Size:7700; Vector Backbone:pLL5.0; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Comments: pLL5.0 derived from pLL3.7; CMV promoter replaced with 5' LTR promoter from pMSCV.

Proper citation: RRID:Addgene_37215 Copy   

•   Source: Addgene

http://www.addgene.org/37216

Species: Mus musculus
Genetic Insert: mouse ZO-1 shRNA
Vector Backbone Description: Backbone Size:7700; Vector Backbone:pLL5.0; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Comments: pLL5.0 derived from pLL3.7; CMV promoter replaced with 5' LTR promoter from pMSCV.

Proper citation: RRID:Addgene_37216 Copy   

•   Source: Addgene

http://www.addgene.org/37176

Genetic Insert: None
Vector Backbone Description: Vector Backbone:pUX3236; Vector Types:; Bacterial Resistance:Ampicillin
Comments: This strain contains Roberts lab plasmid pUX3236 which is pUX2424 (contains ~1200bp fragment from Burkholderia xenovorans LB400 with introduced terminal HindIII and SalI sites [such that HindIII – (<-rcoM1– intergenic region – coxM1′->) – SalI]) but with a higher-affinity “e↑” binding site (5′-TCCTACAGTTCACGCACGT-3′). Suitable template for amplification and mutagenesis. For strain usage see attached pdf. Please note that this strain has not been confirmed by Addgene functionally.

Proper citation: RRID:Addgene_37176 Copy   

•   Source: Addgene

http://www.addgene.org/37177

Species: Burkholderia xenovorans
Genetic Insert: coxM1′::lacZ reporter system with higher-affinity RcoM1 binding site at the "e" position AND RcoM1 with C-term 6xHIS
Vector Backbone Description: Vector Backbone:pUX3242 and pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3242 (pUX2996 with a coxM1′::lacZ reporter system such that ...vector DNA – BamHI (unique site) – higher-affinity binding site at the “e” position (“e↑” binding site = 5′-TCCTACAGTTCACGCACGT-3′) – coxM1′(with unique SalI site)::lacZ-> tfd <-KanR (with 5′-end unique XhoI site) – vector DNA <-SpR...) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag). The strain is CmR, pUX3242 is KanR and SpecR and pUX2410 is AmpR. Can be grown in Amp + Kan. For strain usage see attached pdf.

Proper citation: RRID:Addgene_37177 Copy   

•   Source: Addgene

http://www.addgene.org/37210

Species: canine
Genetic Insert: dog ZO-2 shRNA
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19605556

Proper citation: RRID:Addgene_37210 Copy   

•   Source: Addgene

http://www.addgene.org/37178

Genetic Insert: coxM1′::lacZ reporter system with RcoM binding sites "a-f" spaced at 21bp AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3254 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3254 (coxM1′::lacZ reporter system with binding sites “a-f” with a deletion between the “c” and “d” binding motifs such that the individual binding motifs “a,” “b,” “c,” “d,” “e,” and “f” are spaced at 21-bp intervals) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag). Strain is CmR, pUX3254 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan. For strain usage see attached pdf.

Proper citation: RRID:Addgene_37178 Copy   

•   Source: Addgene

http://www.addgene.org/37211

Species: canine
Genetic Insert: dog ZO-2 shRNA
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19605556

Proper citation: RRID:Addgene_37211 Copy   

•   Source: Addgene

http://www.addgene.org/37179

Genetic Insert: coxM1′::lacZ reporter system of pUX3009 with an improved −35/ −10 RNAP binding region AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3272 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3272 (pUX3009 based coxM1′::lacZ reporter system with binding sites “d + e + f” and an improved −35/−10 RNAP binding region (5′-TTGACA-(18N)-TATCCT-3′) ) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag). Strain is CmR, pUX3272 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan. For strain usage see attached pdf.

Proper citation: RRID:Addgene_37179 Copy   

•   Source: Addgene

http://www.addgene.org/37173

Genetic Insert: ~5-kb BamHI + XhoI fragment from pUX2996 cloned into BamHI + SalI-cut pEXT20
Vector Backbone Description: Vector Backbone:pUX3025; Vector Types:; Bacterial Resistance:Ampicillin
Comments: This strain contains Roberts lab plasmid pUX3025 which contains ~5-kb BamHI + XhoI fragment from pUX2996 cloned into BamHI + SalI-cut pEXT20. Useful for mutagenesis and excision of the “e + f” binding region. For strain usage details see attached pdf. Please note that this strain has not been tested functionally by Addgene.

Proper citation: RRID:Addgene_37173 Copy   

•   Source: Addgene

http://www.addgene.org/37175

Genetic Insert: ~5-kb BamHI + XhoI fragment from pUX3010 cloned into BamHI + SalI-cut pEXT20
Vector Backbone Description: Vector Backbone:pUX3057; Vector Types:; Bacterial Resistance:Ampicillin
Comments: This strain contains Roberts lab plasmid pUX3057 which contains ~5-kb BamHI + XhoI fragment from pUX3010 cloned into BamHI + SalI-cut pEXT20. Useful for mutagenesis and excision of the “a-f” binding region. For strain usage details see attached pdf. Please note that Addgene has not tested this strain functionally.

Proper citation: RRID:Addgene_37175 Copy   

•   Source: Addgene

http://www.addgene.org/37180

Genetic Insert: coxM1′::lacZ reporter system of pUX3009 with “e” binding site “TTnnnG” motif changed to “GGnnnG” AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3297 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3297 (pUX3009 based coxM1′::lacZ reporter system with binding sites “d + e + f” with the “e” binding site “TTnnnG” motif changed to “GGnnnG;”) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag). Strain is CmR, pUX3297 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan. For strain usage see attached pdf.

Proper citation: RRID:Addgene_37180 Copy   

•   Source: Addgene

http://www.addgene.org/37206

Species: canine
Genetic Insert: dog ZO-1 shRNA
Vector Backbone Description: Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19605556

Proper citation: RRID:Addgene_37206 Copy   

•   Source: Addgene

http://www.addgene.org/37169

Genetic Insert: coxM1′::lacZ reporter system with RcoM binding site "f" AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3008 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3008 (coxM1′::lacZ reporter system with binding site “f”) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag). Strain is CmR, pUX3008 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan. For strain usage see attached pdf.

Proper citation: RRID:Addgene_37169 Copy   

•   Source: Addgene

http://www.addgene.org/37202

Species: Homo sapiens
Genetic Insert: A2BR
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21628448
Comments: A2B was PCR amplified as a 1068bp fragment incorporating a 5' EcoRI site. The stop codon was eliminated and a 3' BamHI site was added with the reverse PCR primer to allow direct cloning into pEYFP-N1

Proper citation: RRID:Addgene_37202 Copy   

•   Source: Addgene

http://www.addgene.org/37167

Genetic Insert: coxM1′::lacZ reporter system AND Burkholderia xenovorans RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX2996 and pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX2996 (pUX2970 with a coxM1′::lacZ reporter system such that ...vector DNA – BamHI (unique site) – “e + f” binding region – coxM1′(with unique SalI site)::lacZ-> tfd <-KanR (with 5′-end unique XhoI site) – vector DNA <-SpR...) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag). Strain is CmR, plasmid pUX2996 is KanR and SpecR and plasmid is pUX2410 is AmpR. Can be grown in media containing Amp + Kan. For strain usage see attached pdf. Please note that this strain has not been tested functionally by Addgene.

Proper citation: RRID:Addgene_37167 Copy   

•   Source: Addgene