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Species: Rattus norvegicus
Genetic Insert: REACh-CaMKIIa-mEGFP
Vector Backbone Description: Backbone Size:3927; Vector Backbone:Clontech N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:19295602
Proper citation: RRID:Addgene_26933 Copy
Species: Rattus norvegicus
Genetic Insert: bJunVN155(I152L)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3791; Vector Backbone:pMyc-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21091444
Comments: To be published in Biotechniques. Please suggest users to cite the article later. Also, this plasmid can be used to replace pBiFC-bJunVN173.
Proper citation: RRID:Addgene_27098 Copy
Species: Rattus norvegicus
Genetic Insert: TSC2
Vector Backbone Description: Backbone Size:0; Vector Backbone:N/A; Vector Types:N/A; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_24939 Copy
Species: Rattus norvegicus
Genetic Insert: PAM
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19604476
Proper citation: RRID:Addgene_25458 Copy
Species: Rattus norvegicus
Genetic Insert: PHM
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9283080
Proper citation: RRID:Addgene_25455 Copy
Species: Rattus norvegicus
Genetic Insert: Kal7
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pEAK-His-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10692441
Comments: There are 2 mutations in the sequence, T64S and N80Y. These sequencing errors are the result of mistakes in initial submission to GenBank. The sequence should be correct.
Proper citation: RRID:Addgene_25454 Copy
Species: Rattus norvegicus
Genetic Insert: PAM1
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCIS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1577852
Proper citation: RRID:Addgene_25456 Copy
Species: Rattus norvegicus
Genetic Insert: Kal5
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pEAK-His-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10777487
Comments: Please see depositor's sequence for Kal5 for reference.
Proper citation: RRID:Addgene_25440 Copy
Species: Rattus norvegicus
Genetic Insert: Kal12
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pEAK-His-Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10777487
Comments: There are 2 mutations in the sequence, T46S and N62Y. These sequencing errors are the result of mistakes in initial submission to GenBank by the depositing lab. The sequence should be correct.
Proper citation: RRID:Addgene_25442 Copy
Species: Rattus norvegicus
Genetic Insert: NavII-III
Vector Backbone Description: Backbone Marker:Addgene plasmid 20945; Backbone Size:10093; Vector Backbone:pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21048938
Proper citation: RRID:Addgene_26057 Copy
Species: Rattus norvegicus
Genetic Insert: mammalian target of rapamycin-wild type
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20190810
Proper citation: RRID:Addgene_26036 Copy
Species: Rattus norvegicus
Genetic Insert: p97
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5137; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21822278
Comments: Contains NMHTG linker between myc and Strep tags.
The hygromycin resistance gene in this plasmid lacks a promoter and native start codon. See Invitrogen's website for more information about the vector backbone.
Proper citation: RRID:Addgene_31841 Copy
Species: Rattus norvegicus
Genetic Insert: CD200
Vector Backbone Description: Backbone Marker:Mark Bushell; Backbone Size:6000; Vector Backbone:pEMCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487
Proper citation: RRID:Addgene_32405 Copy
Species: Rattus norvegicus
Genetic Insert: CD200 Receptor extracellular domain
Vector Backbone Description: Backbone Marker:Mark Bushell; Backbone Size:6000; Vector Backbone:pEMCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487
Proper citation: RRID:Addgene_32406 Copy
Species: Rattus norvegicus
Genetic Insert: CD200
Vector Backbone Description: Backbone Marker:Mark Bushell; Backbone Size:6000; Vector Backbone:pEMCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487
Comments: Please note that Addgene's sequencing result with the LNCX primer found a single nucleotide mismatch at bp# 6587 when compared to the full plasmid sequence provided by the depositing scientist. According to the depositing scientist, the mismatch is in the Kozak sequence preceding the CD200bio open reading frame and should not affect protein expression.
Proper citation: RRID:Addgene_32404 Copy
Species: Rattus norvegicus
Genetic Insert: CD200 Receptor extracellular domain
Vector Backbone Description: Backbone Marker:Mark Bushell; Backbone Size:6000; Vector Backbone:pEMCS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487
Proper citation: RRID:Addgene_32407 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18460329
Comments: The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_32452 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18460329
Comments: The S3V, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_32450 Copy
Species: Rattus norvegicus
Genetic Insert: Calcium/Calmodulin dependent protein kinase kinase beta
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4100; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18460329
Comments: The S3L, G96R, and P108L "mutations" were present in the original version of rat CaMKKbeta cloned by the Means lab. They believe these sequence differences represent a natural variant of the CaMKKbeta sequence and are not mutations.
Proper citation: RRID:Addgene_32449 Copy
Species: Rattus norvegicus
Genetic Insert: dominant negative Rheb (D60K)
Vector Backbone Description: Backbone Marker:Addgene plasmid #32519; Vector Backbone:pHAGE-CMV-Rheb-IRES-eGFP-W; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: The published dominant negative D60K mutation was introduced to pHAGE-CMV-Rheb-IRES-eGFP-W by site directed mutagenesis. Although reported to have robust DN activity in other cell types, this construct did not provide robust DN activity in neurons.
The original pHAGE-CMV-IRES lentiviral vector is from Dr Richard C. Mulligan at Harvard (Mostoslavsky G et al., 2005).
Proper citation: RRID:Addgene_32522 Copy
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