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Species: Caenorhabditis elegans
Genetic Insert: Q7JLY3_CAEEL_48-124
Vector Backbone Description: Vector Backbone:YK 02; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35176859
Comments: This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). See supplemental table 2 in our paper to see its performance in our assay. The negative control for this assay is pSecTag2 B which can be obtained from Thermo here https://www.thermofisher.com/order/catalog/product/V90020
Proper citation: RRID:Addgene_185232 Copy
Species: Caenorhabditis elegans
Genetic Insert: Q19228_CAEEL_51-88
Vector Backbone Description: Vector Backbone:YK 02; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35176859
Comments: This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis). See supplemental table 2 in our paper to see its performance in our assay. The negative control for this assay is pSecTag2 B which can be obtained from Thermo here https://www.thermofisher.com/order/catalog/product/V90020
Proper citation: RRID:Addgene_185230 Copy
Species: Caenorhabditis elegans
Genetic Insert: MRP-1
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:4376; Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35513700
Proper citation: RRID:Addgene_184130 Copy
Species: Caenorhabditis elegans
Genetic Insert: genomic mcm-3 fused to mScarlet
Vector Backbone Description: Backbone Marker:Maureen Barr lab (Nikonorova et al 2022); Backbone Size:4358; Vector Backbone:pIN1_GGGSx3_mScarlet; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35334227
Proper citation: RRID:Addgene_182933 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi5605
Vector Backbone Description: Backbone Marker:John Calarco's lab at University of Toronto; Vector Backbone:loxP_myo2_neoR; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36640369
Proper citation: RRID:Addgene_194056 Copy
Species: Caenorhabditis elegans
Genetic Insert: ChrII_ttTi5605 sgRNA
Vector Backbone Description: Vector Backbone:pTK73; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36640369
Comments: An identical plasmid has been reported by Dr. Shinsuke Niwa's group.
doi.org/10.1371/journal.pone.0197128
Proper citation: RRID:Addgene_194058 Copy
Species: Caenorhabditis elegans
Genetic Insert: lim-4
Vector Backbone Description: Vector Backbone:pPD95.62; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33378642
Proper citation: RRID:Addgene_191028 Copy
Species: Caenorhabditis elegans
Genetic Insert: Cbr-unc-119 Punc-122::GFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2544; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20418868
Proper citation: RRID:Addgene_24981 Copy
Species: Caenorhabditis elegans
Genetic Insert: systemic RNA interference-deficient 1
Vector Backbone Description: Backbone Marker:A. Fire; Backbone Size:4000; Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20512143
Comments: Specific details about this vector are given at the Materials and Methods of the article: Calixto et al, 2010, Nat Meth "Enhanced Neuronal RNAi using sid-1"
Note that this plasmid contains genomic sid-1, NOT the cDNA.
Proper citation: RRID:Addgene_25811 Copy
Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729841
Comments: extremely slow growing.
Proper citation: RRID:Addgene_26390 Copy
Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20729841
Proper citation: RRID:Addgene_26386 Copy
Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20729841
Proper citation: RRID:Addgene_26388 Copy
Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20729841
Comments: PLEASE NOTE: this plasmid is approximately 1.6kb shorter than expected in the region between the T7 promoter and pBR322 origin region based on the author sequence. Both promoters are present in their entirety based on the Addgene Quality Control sequencing. According to the depositing laboratory the plasmid behaves as expected and gives the expected pattern of fluorescence and Neomycin resistant phenotype.
There are a few other mismatches with author sequence but they also do not affect function. For questions regarding this plasmid please contact the depositing scientist Denis Dupuy at [email protected]
AgeI is NOT a unique restriction site. A second site is present at around nucleotide 7210.
Proper citation: RRID:Addgene_26393 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7_LPB
Vector Backbone Description: Backbone Size:7556; Vector Backbone:MXW-PGK-let7-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: BamHI and BstBI are endogenous sites that flank the hairpin region of wt cel-let-7.
Proper citation: RRID:Addgene_26548 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7_LPA
Vector Backbone Description: Backbone Size:7556; Vector Backbone:MXW-PGK-let7-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: BamHI and BstBI are endogenous sites that flank the hairpin region of wt cel-let-7.
Proper citation: RRID:Addgene_26547 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-pre-let-7_LPC
Vector Backbone Description: Backbone Size:7556; Vector Backbone:MXW-PGK-let7-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Comments: BamHI and BstBI are endogenous sites that flank the hairpin region of wt cel-let-7.
Proper citation: RRID:Addgene_26549 Copy
Species: Caenorhabditis elegans
Genetic Insert: lin-41_LCS-2G
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:4075; Vector Backbone:phRL-TKlkF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Proper citation: RRID:Addgene_26544 Copy
Species: Caenorhabditis elegans
Genetic Insert: lin-41_LCS-2U
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:4075; Vector Backbone:phRL-TKlkF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Proper citation: RRID:Addgene_26543 Copy
Species: Caenorhabditis elegans
Genetic Insert: cel-let-7 (~250nt flanking each side)
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:7060; Vector Backbone:MXW-PGK-IRES-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Proper citation: RRID:Addgene_26545 Copy
Species: Caenorhabditis elegans
Genetic Insert: lin-41_LCS-wt
Vector Backbone Description: Backbone Marker:Chang-Zheng Chen Lab; Backbone Size:4075; Vector Backbone:phRL-TKlkF; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20808284
Proper citation: RRID:Addgene_26542 Copy
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