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Plasmids are provided by Addgene and DGRC.

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pHSN501
 
Resource Report
Resource Website 		RRID:Addgene_50589 zCas9D10A Synthetic Kanamycin and Spectinomycin PMID:25432517 Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin Cas9D10A nickase, was derived from the zCas9 and included in the toolkit to facilitate HR-based gene replacement or NHEJ-based insertion/deletion events (indels) through offset nicking under the precondition of the avoidance of off-target effects 2026-08-29 01:06:33 0
pCBC-MT1T2
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_50593 T1, gRNA scaffold, OsU3t plus, TaU3p, T2 Synthetic Chloramphenicol PMID:25432517 Please note that this plasmid runs as a dimer (>7kb) or multimer. Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:33 1
pCBC-MT3T4
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_50595 T3, gRNA scaffold, U6-26t plus, OsU3p, T4 Synthetic Chloramphenicol PMID:25432517 Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:33 1
pCBC-DT1T2
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_50590 T1, U626t plus, U629p, T2 Synthetic Chloramphenicol PMID:25432517 Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:33 8
pCBC-DT3T4
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_50592 T3, gRNA scaffold, U6-1tplus, U6-26p, T4 Synthetic Chloramphenicol PMID:25432517 Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol 2026-08-29 01:06:33 3
pBS-3xGFP
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_50659 3xGFP Synthetic Ampicillin PMID:12566428 Please note that this plasmid is unstable due to the tandem yeGFP genes. Screening of colonies is required prior to storage. See diagnostic digest for expected results. Vector Backbone:pBS; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:34 1
pCAG-rTetRDBD-GBP1
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_50791 rTetR DNA binding domain-GBP1 fusion protein Synthetic Ampicillin PMID:23953120 Tang J.C. et al. (2013). A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation. Cell. 2013 Aug 15;154(4):928-39. Backbone Size:4823; Vector Backbone:pCAG-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:35 1
pAcBac1.tR4-MbPyl
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_50832 two-copy Pyl tRNA cassette Synthetic Ampicillin PMID:23818609 Vector Backbone:pAcBac; Vector Types:Insect Expression, baculoviral; Bacterial Resistance:Ampicillin 2026-08-29 01:06:35 8
pCAG-GBP6-10gly-VPminx4
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_50796 GBP6-VPminx4 activation domain fusion protein Synthetic Ampicillin PMID:23953120 Tang J.C. et al. (2013). A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation. Cell. 2013 Aug 15;154(4):928-39. Note that GBP6 was verified in Addgene's quality control sequencing but cannot be displayed. Backbone Size:4823; Vector Backbone:pCAG-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:35 1
pAcBac2.tR4-OMeYRS/GFP*
 
Resource Report
Resource Website 		RRID:Addgene_50831 two-copy Tyr tRNA cassette Synthetic Ampicillin PMID:23818609 Vector Backbone:pAcBac; Vector Types:Insect Expression, baculoviral; Bacterial Resistance:Ampicillin 2026-08-29 01:06:35 0
pCAG-EGxxFP
 
Resource Report
Resource Website
50+ mentions 		RRID:Addgene_50716 split EGFP Synthetic Ampicillin PMID:24284873 Backbone Size:5138; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-29 01:06:34 63
RANLS-DEVD-BNES
 
Resource Report
Resource Website 		RRID:Addgene_50840 ddRFP A and ddRFP B Synthetic Ampicillin PMID:25622108 There is a small discrepancy between the reference sequence and Addgene's quality control sequence, this causes an A to G mutation in the linker region between NLS and ddRFP B. This change should not affect plasmid function. Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:35 0
GANES-DEVD-BNLS
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_50842 ddGFP A and ddGFP B Synthetic Ampicillin PMID:25622108 Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:35 1
NESRA-DEVD-BNLS
 
Resource Report
Resource Website 		RRID:Addgene_50849 ddRFP A and ddRFP B Synthetic Ampicillin PMID:25622108 Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:35 0
GANLS
 
Resource Report
Resource Website 		RRID:Addgene_50852 ddGFP A Synthetic Ampicillin PMID:25622108 Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-29 01:06:35 0
pLKO.1-puro U6 sgRNA CAG
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_50927 negative control sgRNA synthetic Ampicillin PMID:24346702 Please note that Addgene's diagnostic digest results suggest that the backbone may be longer than suggested. Backbone Size:7145; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin 2026-08-29 01:06:36 4
pAAV-CaMKIIa-oChIEF(E163A/T199C)-P2A-mKate2
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_51092 oChIEF(E163A/T199C) Synthetic Ampicillin The addition of the E163A and T199C mutations in oChIEF impart higher photocurrent amplitude while retaining fast kinetic properties. This variant is proposed as an alternative to ChETA variants that exhibit smaller photocurrents and more toxicity. Backbone Marker:custom; Backbone Size:5100; Vector Backbone:pAAV-CaMKIIa-WPRE-HGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin E163A/T199C 2026-08-29 01:06:37 1
AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpA
 
Resource Report
Resource Website 		RRID:Addgene_51095 ChIEF(E162A/T198C) Synthetic Ampicillin The addition of the E162A and T198C mutations in ChIEF impart higher photocurrent amplitude while retaining fast kinetic properties. This variant is proposed as an alternative to ChETA variants that exhibit smaller photocurrents and more toxicity. Backbone Marker:custom; Backbone Size:1047; Vector Backbone:pAAV-EF1a-DIO-WPRE-BGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin E162A/T198C 2026-08-29 01:06:37 0
pICSL11024 (pICH47732::NOSp-NPTII-OCST)
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_51144 NOSp-NPTII-OCST Synthetic Ampicillin Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pICH47732; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-29 01:06:37 22
pICSL11017 (pICH47732::NOSp-BAR-NOST)
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_51145 NOSp-BAR-NOST Synthetic Ampicillin Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pICH47732; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-29 01:06:37 1

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