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Species: E. coli
Genetic Insert: Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184982 Copy
Species: E. coli
Genetic Insert: Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184981 Copy
Species: E. coli
Genetic Insert: Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pET-21(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184960 Copy
Species: E. coli
Genetic Insert: Eco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184966 Copy
Species: E. coli
Genetic Insert: Integrating inducible cassette: Eco1 RT
Vector Backbone Description: Vector Backbone:pZS157 (Addgene plasmid #114454); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184970 Copy
Species: E. coli
Genetic Insert: Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184979 Copy
Species: E. coli
Genetic Insert: RNase III
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36493370
Proper citation: RRID:Addgene_189537 Copy
Species: E. coli
Genetic Insert: MG1655-C321 mutS+, λ-, Δ(ybhB-bioAB)::zeoR, ΔprfA, ΔserB
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:36280721
Comments: MG1655-C321 -- All 321 UAG codons changed to UAA.
mutS+ -- Restores normal spontaneous mutation frequency.
(λ-) – Deleted Lambda Phage operon to allow growth at 37C.
Δ(ybhB-bioAB)::zeoR – Deletion of biotin biosynthetic operon using Zeocin cassette; strain is d-biotin auxotroph.
ΔprfA – Deletion of polypeptide release factor 1 (RF1) to prevent translation termination at UAG codons.
ΔserB – Deletion of phosphoserine phosphatase to provide intracellular source of phosphoserine; strain is Serine auxotroph.
The genome sequence of a similar strain (C321.deltaA) was deposited at NCBI (accession # CP006698.1)
Proper citation: RRID:Addgene_192872 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191620 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191621 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191627 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191625 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191629 Copy
Species: E. coli
Genetic Insert: wt E. coli SSB
Vector Backbone Description: Backbone Marker:EMB/Novagen; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24021816
Proper citation: RRID:Addgene_197260 Copy
Species: E. coli
Genetic Insert: bacterial RNaseHI
Vector Backbone Description: Vector Backbone:pLVX; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36544021
Proper citation: RRID:Addgene_196701 Copy
Species: E. coli
Genetic Insert: MscL(G22S)-c-term-cpGFP-ERexp
Vector Backbone Description: Backbone Size:6100; Vector Backbone:Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36608338
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.04.510821v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_195346 Copy
Species: E. coli
Genetic Insert: acrAB
Vector Backbone Description: Backbone Size:3658; Vector Backbone:pBbA5k; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30310093
Proper citation: RRID:Addgene_195516 Copy
Species: E. coli
Genetic Insert: RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9121589
Comments: This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids:
http://www.addgene.org/browse/article/8393/
The following articles can provide more information on using the bacterial 2-hybrid system:
Dove, et al. 1997 PMID 9121589
Dove and Hochschild 1998 PMID 9499408
Deaconescu, et al. 2006 PMID 16469698
Deighan, et al. 2008 PMID 18832144
Kuznedelov, et al. 2002 PMID 11823642
Proper citation: RRID:Addgene_53732 Copy
Species: E. coli
Genetic Insert: ykgMO-IGS
Vector Backbone Description: Backbone Marker:p15A ori plasmid; Backbone Size:4770; Vector Backbone:pJBL5034; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37523538
Proper citation: RRID:Addgene_207231 Copy
Species: E. coli
Genetic Insert: ykgMO-IGS
Vector Backbone Description: Backbone Marker:p15A ori plasmid; Backbone Size:2269; Vector Backbone:pJBL5034; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:37523538
Proper citation: RRID:Addgene_207232 Copy
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