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Species: Homo sapiens
Genetic Insert: Human SMN1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16301532
Proper citation: RRID:Addgene_37057 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid #37060; Vector Backbone:pHSS6; Vector Types:; Bacterial Resistance:Kanamycin
Comments: Used to create strains to complement mutations using the NICS system (Mehr and Seifert, 1998; Mehr, 2000). Insert a functional copy of the gene ectopically at an unlinked chromosomal locus between the lctP and aspC genes under control of lac regulatory sequences, and linked to an Erythromycin-resistance cassette.
Proper citation: RRID:Addgene_37058 Copy
Species: Homo sapiens
Genetic Insert: Smad2
Vector Backbone Description: Backbone Marker:Weinberg Lab; Backbone Size:6900; Vector Backbone:pLKO.1-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20086175
Proper citation: RRID:Addgene_37051 Copy
Vector Backbone Description: Backbone Size:2320; Vector Backbone:pMB8; Vector Types:Cloning vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:3003748
Proper citation: RRID:Addgene_37060 Copy
Species: Homo sapiens
Genetic Insert: HCF-1 shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6349; Vector Backbone:pSuper.Retro.Puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22371484
Proper citation: RRID:Addgene_36997 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid #1764; Backbone Size:4517; Vector Backbone:pBABE-Puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22371484
Proper citation: RRID:Addgene_36999 Copy
Species: Homo sapiens
Genetic Insert: THAP11 shRNA
Vector Backbone Description: Backbone Marker:OligoEngine; Backbone Size:6349; Vector Backbone:pSuper.Retro.Puro; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22371484
Proper citation: RRID:Addgene_36993 Copy
Species: Homo sapiens
Genetic Insert: MAML1(1-990)
Vector Backbone Description: Vector Backbone:pHAGE-N-V5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22547818
Proper citation: RRID:Addgene_37049 Copy
Species: Homo sapiens
Genetic Insert: Smad7
Vector Backbone Description: Backbone Marker:Weinberg Lab; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20086175
Comments: Insert was blunt-ended and ligated into SnaBI-digested pBabe-puro vector.
Proper citation: RRID:Addgene_37044 Copy
Species: Homo sapiens
Genetic Insert: Dsg2
Vector Backbone Description: Backbone Marker:K. Green lab; Vector Backbone:pmCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22184201
Proper citation: RRID:Addgene_36991 Copy
Species: Homo sapiens
Genetic Insert: Smad4
Vector Backbone Description: Backbone Marker:Weinberg Lab; Backbone Size:6900; Vector Backbone:pLKO.1-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20086175
Proper citation: RRID:Addgene_37046 Copy
Species: Homo sapiens
Genetic Insert: Dsc2
Vector Backbone Description: Backbone Marker:GE Healthcare; Vector Backbone:PGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22184201
Proper citation: RRID:Addgene_36992 Copy
Species: Bovine Papillomavirus
Genetic Insert: BPV1 E6
Vector Backbone Description: Vector Backbone:N-Tap-Flag-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22547818
Proper citation: RRID:Addgene_37047 Copy
Species: Homo sapiens
Genetic Insert: TGFβRII
Vector Backbone Description: Backbone Marker:Weinberg Lab; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21245094
Comments: Insert was blunt-ended and ligated into SnaBI-digested pBabe-puro.
Proper citation: RRID:Addgene_37040 Copy
Species: Homo sapiens
Genetic Insert: Smad4
Vector Backbone Description: Backbone Marker:Weinberg Lab; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21245094
Comments: Insert was blunt-ended and ligated into SnaBI-digested pBabe-puro vector
Proper citation: RRID:Addgene_37041 Copy
Species: Homo sapiens
Genetic Insert: MAML1-ADDAA
Vector Backbone Description: Vector Backbone:pHAGE-N-V5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22547818
Comments: MAML1-ADDAA acidic domain mutant
Proper citation: RRID:Addgene_37050 Copy
Species: Mus musculus
Genetic Insert: slug
Vector Backbone Description: Backbone Marker:Dr. Tal Kafri; Backbone Size:0; Vector Backbone:pTK380 (tetracycline inducible); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: Slug cDNA was PCR amplified from an IMAGE clone (Open Biosystems) and cloned into BamHI-XbaI sites of pTK380.
Proper citation: RRID:Addgene_36986 Copy
Species: Homo sapiens
Genetic Insert: Dsg2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-N2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18987342
Comments: The construct encoding human Dsg2 cDNA in pBluescript was a gift from Drs. W. Franke and S. Schafer, German Cancer Research Center, Heidelberg, Germany.
Proper citation: RRID:Addgene_36989 Copy
Species: Mus musculus
Genetic Insert: Hes1
Vector Backbone Description: Backbone Marker:Trono lab (Addgene plasmid 12257); Backbone Size:10510; Vector Backbone:pWPXL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: The mouse Hes1 cDNA was cloned between MluI and SpeI sites (blunted) in pWPXL. This resulted in replacement of the EGFP in the vector backbone with mouse Hes1.
Proper citation: RRID:Addgene_36983 Copy
Species: Homo sapiens
Genetic Insert: beta-catenin deltaN90
Vector Backbone Description: Backbone Marker:I. Ben-Porath; Backbone Size:0; Vector Backbone:pLV-IRES-neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: The pLV-neo vector was modified from the attached vector by replacing dsRed with Neomycin resistant cassette and removing tTR-kRAB.
Addgene QC sequence analysis identified a S374G discrepancy, relative to reference sequence NP_001091679.1.
Proper citation: RRID:Addgene_36985 Copy
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