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Species: Homo sapiens
Genetic Insert: cullin 7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4620; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20696 Copy
Species: Homo sapiens
Genetic Insert: cullin 7
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5486; Vector Backbone:pcDNA3-myc3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20695 Copy
Species: Artificial
Genetic Insert: GFP-NLS-lacZ
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5352; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20701 Copy
Species: Mus musculus
Genetic Insert: pCMV5 Flag p38 alpha (agf)
Vector Backbone Description: Backbone Marker:Dr. David Russell, UTSW; Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20787 Copy
Species: artificial gene
Genetic Insert: 4 finger ZFP
Vector Backbone Description: Backbone Size:3710; Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20704 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2513; Vector Backbone:pFA6a; Vector Types:Yeast Expression, PCR-based C-terminal tagging; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20780 Copy
Species: Mus musculus
Genetic Insert: pCMV5 Flag p38 alpha
Vector Backbone Description: Backbone Marker:Dr. David Russell, UTSW; Backbone Size:4657; Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20786 Copy
Species: Mus musculus
Genetic Insert: pDCNA3 Flag p38 delta
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20785 Copy
Species: Homo sapiens
Genetic Insert: CAND1 (cullin-associated and neddylation-dissociated 1)
Vector Backbone Description: Backbone Size:5512; Vector Backbone:pcDNA3-HA2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20719 Copy
Species: Mus musculus
Genetic Insert: beta-transducin repeat containing (BTRC)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5486; Vector Backbone:pcDNA3-myc3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: *possible Y377I mutation in stock. This mutation is not known to affect function.
Proper citation: RRID:Addgene_20718 Copy
Species: Homo sapiens
Genetic Insert: Moesin
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20679 Copy
Species: Homo sapiens
Genetic Insert: RING finger protein 1 (ROC1)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4620; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20717 Copy
Species: Danio rerio
Genetic Insert: mir-206-1/mir-133b
Vector Backbone Description: Backbone Marker:Bartel Lab; Backbone Size:0; Vector Backbone:pBSII SK+ modified to introduce GFP and more cloning sites; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
References:
Comments: For polycistronic mir-206-1/mir-133b, we were not able to identify the transcriptional start precisely by 5′-RACE due to the repetitive sequence in this region. Analyses of EST clones (BM259633, CF999541, CF348604, DR729161) mapped the approximate start of the mir-206-1/mir-133b primary transcript to 1.4 kb from the mir-206 hairpin. A 4.5-kb upstream fragment that includes ∼0.9 kb of the primary transcript was amplified from genomic DNA using primers 3 and 4 (5'-CTGCCCCATTTAGACTCTTTCTTGACG-3' and 5'-CCAGAAGAACCCAGACGAAACCCAC-3'). The promoter fragment was fused to a GFP reporter gene. The miRNA promoter fusion was flanked by I-SceI meganuclease recognition sites to increase the efficiency of transgenensis.
Please note that Addgene's sequencing results show some differences from the published sequence. The depositing lab is aware of the differences.
Proper citation: RRID:Addgene_20793 Copy
Species: Danio rerio
Genetic Insert: mir-124
Vector Backbone Description: Backbone Marker:Bartel Lab; Backbone Size:0; Vector Backbone:pBSII SK+ modified to introduce GFP and more cloning sites; Vector Types:zebrafish expression; Bacterial Resistance:Ampicillin
References:
Comments: 5′-RACE (GenRacer kit, Invitrogen) identified a transcriptional start of the mir-124-5 primary transcript, which is located 1.1 kb from the mir-124-5 hairpin. A 5.6-kb fragment upstream of the mir-124-5 transcriptional start was amplified from genomic DNA using primers 1 and 2 (5'-TGCTTTGAGGCACGGTTATGGTCCC-3' and 5'-CTCGGGCAGCGGCTTTTTGGTCCTG-3'). The promoter fragment was fused to a GFP reporter gene. The miRNA promoter fusion was flanked by I-SceI meganuclease recognition sites to increase the efficiency of transgenensis.
Proper citation: RRID:Addgene_20792 Copy
Species: Homo sapiens
Genetic Insert: VHL
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4959; Vector Backbone:pGex2TK; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20790 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2513; Vector Backbone:pFA6a; Vector Types:Yeast Expression, PCR-based C-terminal tagging; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20768 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2513; Vector Backbone:pFA6a; Vector Types:Yeast Expression, PCR-based C-terminal tagging; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20767 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2513; Vector Backbone:pFA6a; Vector Types:Yeast Expression, PCR-based C-terminal tagging; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20763 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2513; Vector Backbone:pFA6a; Vector Types:Yeast Expression, PCR-based C-terminal tagging; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20762 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2513; Vector Backbone:pFA6a; Vector Types:Yeast Expression, PCR-based C-terminal tagging; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20761 Copy
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