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  • Organism:e. coli (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pAT-9251
 
Resource Report
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RRID:Addgene_124226 SpCas9-HF1 E. coli Chloramphenicol PMID:33450024 Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol 2026-08-15 01:04:04 0
3XFlag-pA-Tn5-Fl
 
Resource Report
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10+ mentions
RRID:Addgene_124601 Protein A and hyperactive Tn5 transposase (Tnp) fusion protein E. coli Ampicillin PMID:31036827 Please note that verifying this plasmid by sequencing or digest is challenging due to the presence of the helper plasmid, as multiple bands or mispriming is likely to occur. See Addgene's sequencing results to compare sequences. This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but can reduce transformation or transfection efficiencies. If you have trouble isolating the monomeric version of this plasmid, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid. Backbone Size:8079; Vector Backbone:pTXB1-Tn5; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:08 32
pBad-HPII
 
Resource Report
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RRID:Addgene_105839 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:00:55 0
pBad-HPII TAG348
 
Resource Report
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RRID:Addgene_105844 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin TAG stop codon at site 348 2026-08-15 01:00:56 0
pBad-HPII TAG568
 
Resource Report
Resource Website
RRID:Addgene_105845 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin TAG stop codon at site 568 2026-08-15 01:00:56 0
pBad-HPII TAG283
 
Resource Report
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RRID:Addgene_105843 hydroperoxidase II catalase E. coli Ampicillin Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual. Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin TAG stop codon at site 283 2026-08-15 01:01:00 0
pTW122
 
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RRID:Addgene_115962 Lvl0 5'-2xTetApt-mVenus-PEST E. coli Ampicillin PMID:30327560 Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:02:38 0
pTW120
 
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RRID:Addgene_115947 Lvl0 TetR SapI/BsaI Mut E. coli Ampicillin PMID:30327560 Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:02:38 0
pLPT234
 
Resource Report
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1+ mentions
RRID:Addgene_127855 bla E. coli Ampicillin PMID:31604953 This plasmid is a derivative of repressilator plasmids presented in: Potvin-Trottier et al, 2016, Nature. Backbone Size:3000; Vector Backbone:pSC101; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin 2026-08-15 01:04:40 1
pTrc99S-ssDsbA-MBP-4xDQNAT
 
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RRID:Addgene_128398 Maltose binding protein E. coli Spectinomycin and Streptomycin PMID:33536221 Depositor confirms mutation Y342H does not affect plasmid function. Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin Y342H- please see depositor comments 2026-08-15 01:04:46 0
pJL1-MBP-4xDQNAT
 
Resource Report
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RRID:Addgene_128390 Maltose binding protein E. coli Kanamycin PMID:33536221 Depositor confirms mutation does not affect plasmid function. Backbone Size:1765; Vector Backbone:pJL1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin A302T- please see depositor comment 2026-08-15 01:04:46 0
pJ211_rha_WT_hi
 
Resource Report
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RRID:Addgene_128853 Flagellin E. coli Kanamycin PMID:33381753 Backbone Marker:ATUM; Backbone Size:2500; Vector Backbone:pJ211; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:04:51 0
pJ211_rha_WT_low
 
Resource Report
Resource Website
RRID:Addgene_128851 Flagellin E. coli Kanamycin PMID:33381753 Backbone Marker:ATUM; Backbone Size:2500; Vector Backbone:pJ211; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:04:51 0
pJ291_WT_med
 
Resource Report
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RRID:Addgene_128856 Flagellin E. coli Kanamycin PMID:33381753 Backbone Marker:ATUM; Backbone Size:4000; Vector Backbone:pJ291; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:04:52 0
pJ211_WT_med
 
Resource Report
Resource Website
RRID:Addgene_128849 Flagellin E. coli Kanamycin PMID:33381753 Backbone Marker:ATUM; Backbone Size:2500; Vector Backbone:pJ211; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:04:51 0
pJ211_WT_low
 
Resource Report
Resource Website
RRID:Addgene_128848 Flagellin E. coli Kanamycin PMID:33381753 Backbone Marker:ATUM; Backbone Size:2500; Vector Backbone:pJ211; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:04:51 0
pACYCDuet-1_Ec_rpoZ
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_128837 rpoZ E. coli Chloramphenicol PMID:23389035 Backbone Size:3939; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:04:51 4
pEcRNAP6
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_128940 RpoA E. coli Ampicillin PMID:21416542 Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:04:53 4
pCMV Lex VP16 (F442A) HA (P#1710)
 
Resource Report
Resource Website
RRID:Addgene_14595 LexA E. coli Ampicillin PMID:15203006 Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin The VP16 sequence contains an F442A mutation. The substitution of alanine for phenylanine at position 442 has previously been shown to affect the transcriptional activity of VP16. 2026-08-15 01:07:02 0
pCMV Ub Lex VP16 (F442A) HA (P#1711)
 
Resource Report
Resource Website
RRID:Addgene_14596 LexA E. coli Ampicillin PMID:15203006 Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin The VP16 sequence contains an F442A mutation. The substitution of alanine for phenylanine at position 442 has previously been shown to affect the transcriptional activity of VP16. 2026-08-15 01:07:02 0

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