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Species: Danio rerio
Genetic Insert: CaSR-EGFP-SV40
Vector Backbone Description: Backbone Marker:Berger and Currie 2013; Backbone Size:7480; Vector Backbone:pDesttol2pACrymCherry (Addgene #64023); Vector Types:creation of transgenic fish using Tol2 transgenesis method; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36476852
Proper citation: RRID:Addgene_191500 Copy
Species: Danio rerio
Genetic Insert: BleoR-miR-430-G-G mm
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Fish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29272705
Comments: This plasmid was used for IVT-template generation. Mutations may exist within the pre-existing CMV IE94 promoter.
Proper citation: RRID:Addgene_221728 Copy
Species: Danio rerio
Genetic Insert: BleoR-miR-430-10-11 mm
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Fish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29272705
Comments: This plasmid was used for IVT-template generation. Mutations may exist within the pre-existing CMV IE94 promoter.
Proper citation: RRID:Addgene_221727 Copy
Species: Danio rerio
Genetic Insert: kcnk4b
Vector Backbone Description: Backbone Size:2605; Vector Backbone:pENTR-D-TOP; Vector Types:Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38270285
Proper citation: RRID:Addgene_213123 Copy
Species: Danio rerio
Genetic Insert: mCherry-Rab7
Vector Backbone Description: Vector Backbone:pDest; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36044568
Proper citation: RRID:Addgene_213738 Copy
Species: Danio rerio
Genetic Insert: lamp1b-mCherry
Vector Backbone Description: Vector Backbone:pDest; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36044568
Proper citation: RRID:Addgene_213739 Copy
Species: Danio rerio
Genetic Insert: mCherry-Rab5c
Vector Backbone Description: Vector Backbone:pDest; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36044568
Proper citation: RRID:Addgene_213737 Copy
Species: Danio rerio
Genetic Insert: lamp2-mCherry
Vector Backbone Description: Vector Backbone:pDest; Vector Types:Zebrafish; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36044568
Proper citation: RRID:Addgene_213740 Copy
Species: Danio rerio
Genetic Insert: nanos2
Vector Backbone Description: Backbone Marker:Christian Mosimann; Backbone Size:4974; Vector Backbone:pTol2Dest_pCY; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Comments: Cloned by Maya Pahima.
nos2 control elements were cloned from zebrafish ovary gDNA.
Gateway recombination: pTol2-nos2L:d2egfp-nos2,3’utr, cryaa:Venus (pCY) was generated by combining p5E-nos2promL, pME-d2GFP (Collery and Link, 2011), and pCR4-nos2,3’utr (Chapter 6) into the pTol2-R4/R3_cryaa:Venus backbone vector (Mosimann et al., 2013).
Proper citation: RRID:Addgene_226801 Copy
Species: Danio rerio
Genetic Insert: Ace2N-mNeon
Vector Backbone Description: Vector Backbone:pDestTol pA2; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42109300
Comments: Please visit https://doi.org/10.1101/2024.12.12.628143 for bioRxiv preprint.
Proper citation: RRID:Addgene_253618 Copy
Species: Danio rerio
Genetic Insert: nanos2
Vector Backbone Description: Backbone Marker:Florence Marlow, Amanda Heim; Backbone Size:6946; Vector Backbone:pTol2Dest_pBH; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Comments: Cloned by Maya Pahima.
pTol2-nos2L:mApple, cmlc2:dsred (pBH) was generated by combining p5E-nos2prom and pME-mApple (Addgene, 195960) (Kemmler et al., 2023) into the pTol2-R4/R2_cmlc2:dsred (pBH) backbone vector (Heim et al., 2014).
Proper citation: RRID:Addgene_226797 Copy
Species: Danio rerio
Genetic Insert: nanos2
Vector Backbone Description: Backbone Marker:Florence Marlow, Amanda Heim; Backbone Size:6946; Vector Backbone:pTol2Dest_pBH; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Comments: Cloned by Maya Pahima.
pTol2-nos2:emGFP, cmlc2:dsred (pBH) was generated by combining p5E-nos2prom and pME-emGFP into the pTol2-R4/R2_cmlc2:dsred (pBH) backbone vector (Heim et al., 2014).
Proper citation: RRID:Addgene_226798 Copy
Species: Danio rerio
Genetic Insert: miR-21-1-Dendra2
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:41582686
Comments: Please visit https://doi.org/10.1101/2025.07.19.665686 for the bioRxiv preprint.
Proper citation: RRID:Addgene_253623 Copy
Species: Danio rerio
Genetic Insert: vgll4l
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Zebrafish Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_254515 Copy
Species: Danio rerio
Genetic Insert: kcnh5b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pENTR-D-sp6; Vector Types:Mammalian Expression, Gateway Cloning, In vitro transcription for riboprobes; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42496271
Proper citation: RRID:Addgene_255308 Copy
Species: Danio rerio
Genetic Insert: kcnh5a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pENTR-D-sp6; Vector Types:Mammalian Expression, Gateway Cloning, In vitro transcription for riboprobes; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42496271
Proper citation: RRID:Addgene_255307 Copy
Species: Danio rerio
Genetic Insert: kcnh2a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pENTR-D-sp6; Vector Types:Mammalian Expression, Gateway Cloning, In vitro transcription for riboprobes; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42496271
Proper citation: RRID:Addgene_255302 Copy
Species: Danio rerio
Genetic Insert: kcnh7a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pENTR-D-sp6; Vector Types:Mammalian Expression, Gateway Cloning, In vitro transcription for riboprobes; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42496271
Proper citation: RRID:Addgene_255311 Copy
Species: Danio rerio
Genetic Insert: kcnh6b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pENTR-D-sp6; Vector Types:Mammalian Expression, Gateway Cloning, In vitro transcription for riboprobes; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42496271
Proper citation: RRID:Addgene_255310 Copy
Species: Danio rerio
Genetic Insert: kcnh8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pENTR-D-sp6; Vector Types:Mammalian Expression, Gateway Cloning, In vitro transcription for riboprobes; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42496271
Proper citation: RRID:Addgene_255313 Copy
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