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Species: Synthetic
Genetic Insert: EBFP2-NES
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184461 Copy
Species: Synthetic
Genetic Insert: TagRFP-NES
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184449 Copy
Species: Synthetic
Genetic Insert: TagRFP-CAAX
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184447 Copy
Species: Synthetic
Genetic Insert: iRFP702-CAAX
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184455 Copy
Species: Synthetic
Genetic Insert: mCardinal-NES
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184453 Copy
Species: Synthetic
Genetic Insert: iRFP702-LaminB1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184456 Copy
Species: Synthetic
Genetic Insert: iRFP702-NES
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184457 Copy
Vector Backbone Description: Backbone Size:9662; Vector Backbone:pBAC2015, pQS-gusA, and pUC19; Vector Types:; Bacterial Resistance:Hygromycin
Defining Citation: PMID:39666857
Proper citation: RRID:Addgene_227508 Copy
Genetic Insert: Cas9
Vector Backbone Description: Vector Backbone:pGM1190; Vector Types:CRISPR; Bacterial Resistance:Hygromycin
Defining Citation: PMID:39666857
Proper citation: RRID:Addgene_227582 Copy
Species: Other
Genetic Insert: gRNA1(gsdA)
Vector Backbone Description: Backbone Marker:Michael Sauer (Sarkari et al. 2017) Addgene #90278; Vector Backbone:pMST665_BB3_L 23_gRNAempty_cas9_BbsI; Vector Types:Bacterial Expression, CRISPR, A. niger; Bacterial Resistance:Hygromycin
Proper citation: RRID:Addgene_222719 Copy
Genetic Insert: scbR
Vector Backbone Description: Backbone Size:5715; Vector Backbone:pSET152; Vector Types:Bacterial Expression, Synthetic Biology, Integrative into Streptomyces genome via serine integrase (like parent pSET152); Bacterial Resistance:Hygromycin
Defining Citation: PMID:29130169
Comments: Biarnes-Carrera, Marc, Chang Kwon Lee, Takuya Nihira, Rainer Breitling, and Eriko Takano. ‘Orthogonal Regulatory Circuits for Escherichia Coli Based on the γ-Butyrolactone System of Streptomyces Coelicolor’. ACS Synthetic Biology 7, no. 4 (2018): 1043–55. https://doi.org/10.1021/acssynbio.7b00425.
pTE1062 is replicative in E. coli via pMB1 type origin, and carrying oriT (RP4) for delivery to Streptomyces by conjugation. In Streptomyces, pTE1062 is not replicative, and therefore selection is on integration into the genome mediated by phiC31 integrase.
Proper citation: RRID:Addgene_172356 Copy
Genetic Insert: pmyc1tetO
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:17483222
Comments: pmyc1tetO is a tet operator containing promoter for mycobacteria. Also see Pubmed IDs 18059281 and 15687379.
Derivative of pMS2 containing Pmyc1tetO. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.
Proper citation: RRID:Addgene_17972 Copy
Species: A. victoria
Genetic Insert: Ptac::GFP-mut-2
Vector Backbone Description: Backbone Size:6050; Vector Backbone:pJN105; Vector Types:Bacterial Expression, Bacterial broad host range vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:15271914
Comments: The pBBR1MCS-5 derivative of the Bordetella bronchiseptica BHR plasmid has a pBBR/BHR replicon that is maintained in L. pneumophila and is compatible with IncP, IncW, IncQ, and IncRi incompatibility groups. Plasmid pJN105 is a derivative of pBBR1MCS-5 with araC and PBAD regions before the polylinker. PCR primers with BglII sites at their 5′ ends were used to amplify the region outside the gentamicin cassette in pJN105. The PCR fragment was digested with BglII, blunted with the Klenow fragment, and ligated to the blunted DdeI-DdeI hygromycin resistance fragment from pMV206.hygro to form pJN105-hygro. The Ptac-GFP fragment of pGS-GFP04 was amplified by PCR by using primers with NheI sites at their 5′ ends. After digestion with NheI, this fragment was ligated with NheI-digested pJN105-hygro to form pJN105-hygro-GFP.
Proper citation: RRID:Addgene_41883 Copy
Vector Backbone Description: Backbone Marker:Daugelat et al 2003; Backbone Size:6900; Vector Backbone:pSD26; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33006405
Comments: Vector constructed by Dr. Arie Geerlof at EMBL-Hamburg (now at Helmholtz Zentrum München)
Proper citation: RRID:Addgene_42191 Copy
Genetic Insert: dCas9
Vector Backbone Description: Backbone Marker:Chunbo Lou; Backbone Size:6468; Vector Backbone:pTHS; Vector Types:CRISPR, Integrative vector for use in Streptomyces; Bacterial Resistance:Hygromycin
Defining Citation: PMID:37801665
Proper citation: RRID:Addgene_210387 Copy
Genetic Insert: this strain is a P2 lysogen derived from E. coli EMG C5545 strain
Vector Backbone Description: Vector Backbone:This is a strain.; Vector Types:; Bacterial Resistance:Hygromycin
Defining Citation: PMID:36731126
Proper citation: RRID:Addgene_197132 Copy
Species: Synthetic
Genetic Insert: EBFP2-LaminB1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184460 Copy
Species: Synthetic
Genetic Insert: iRFP702-3xNLS
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184454 Copy
Species: Synthetic
Genetic Insert: mCardinal-LaminB1
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184452 Copy
Species: Synthetic
Genetic Insert: mCardinal-3xNLS
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6491; Vector Backbone:pVITRO1-hygro-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34838160
Proper citation: RRID:Addgene_184450 Copy
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