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Species: Rattus norvegicus
Genetic Insert: Fidgetin
Vector Backbone Description: Vector Backbone:C1 Cloning Vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_62756 Copy
Species: Rattus norvegicus
Genetic Insert: AP180
Vector Backbone Description: Vector Backbone:pQE-31; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7814377
Proper citation: RRID:Addgene_63006 Copy
Species: Rattus norvegicus
Genetic Insert: Epsin 1 without ENTH domain
Vector Backbone Description: Backbone Marker:Qiagen; Vector Backbone:pQE-32; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11756460
Proper citation: RRID:Addgene_63008 Copy
Species: Rattus norvegicus
Genetic Insert: Endolyn
Vector Backbone Description: Backbone Marker:Girotti and Banting, PMID 9013339 ; Vector Backbone:delta pMEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10620506
Proper citation: RRID:Addgene_62962 Copy
Species: Rattus norvegicus
Genetic Insert: lgp120
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: The 10 amino acid linker was designed using the sequence as described below but with different cloning sites (EcoRI and BamHI):
Patterson GH and Lippincott-Schwartz J. (2002) A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873-1877.
Examples of use:
Jahreiss L, Menzies FM, Rubinsztein DC. (2008) The itinerary of autophagosomes: from peripheral formation to kiss-and-run fusion with lysosomes. Traffic 9: 574-587.
Renna M, Schaffner C, Winslow AR, Menzies FM, Peden AA, Floto RA, Rubinsztein DC. (2011) Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex. J Cell Sci 124:469-482.
Ogunbayo OA, Zhu Y, Shen B, Agban E, Li J, Ma J, Zhu MX, Evans AM. (2015) Organelle-specific Subunit Interactions of the Vertebrate Two-pore Channel Family. J. Biol. Chem. 290: 1086-1095.
Proper citation: RRID:Addgene_62963 Copy
Species: Rattus norvegicus
Genetic Insert: CathespinB
Vector Backbone Description: Backbone Size:4750; Vector Backbone:EBFP2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:2445929
Comments: . Excitation = 383; Emission = 448
Proper citation: RRID:Addgene_55236 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mAzurite; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: R. norvegicus (rat) Alpha-1 Connexin 43. Excitation = 384; Emission = 450
Proper citation: RRID:Addgene_55231 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:EBFP2; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 383; Emission = 448
Proper citation: RRID:Addgene_55246 Copy
Species: Rattus norvegicus
Genetic Insert: CathespinB
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCerulean; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 433; Emission = 475
Proper citation: RRID:Addgene_55354 Copy
Species: Rattus norvegicus
Genetic Insert: Cx26
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCerulean; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: R. norvegicus (rat) Beta-2 Connexin 26. Excitation = 433; Emission = 475
Proper citation: RRID:Addgene_55361 Copy
Species: Rattus norvegicus
Genetic Insert: CEBPA
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCerulean3-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21479270
Comments: . Excitation = 433; Emission = 475
Proper citation: RRID:Addgene_55407 Copy
Species: Rattus norvegicus
Genetic Insert: Cx43
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCerulean3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21479270
Comments: R. norvegicus (rat) Alpha-1 Connexin 43. Excitation = 433; Emission = 475
Proper citation: RRID:Addgene_55411 Copy
Species: Rattus norvegicus
Genetic Insert: Cx26
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCerulean3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21479270
Comments: R. norvegicus (rat) Beta-2 Connexin 26. Excitation = 433; Emission = 475
Proper citation: RRID:Addgene_55410 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCerulean; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22635056
Comments: . Excitation = 433; Emission = 475
Proper citation: RRID:Addgene_55382 Copy
Species: Rattus norvegicus
Genetic Insert: CEBPA
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTFP1-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18325109
Comments: . Excitation = 462; Emission = 492
Proper citation: RRID:Addgene_55469 Copy
Species: Rattus norvegicus
Genetic Insert: Cx26
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTFP1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18325109
Comments: R. norvegicus (rat) Beta-2 Connexin 26. Excitation = 462; Emission = 492
Proper citation: RRID:Addgene_55473 Copy
Species: Rattus norvegicus
Genetic Insert: LAMP1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mCerulean3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21479270
Comments: . Excitation = 433; Emission = 475
Proper citation: RRID:Addgene_55428 Copy
Species: Rattus norvegicus
Genetic Insert: LC3
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_55579 Copy
Species: Rattus norvegicus
Genetic Insert: CEBPA
Vector Backbone Description: Backbone Size:4750; Vector Backbone:mTurquoise-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 434; Emission = 474
Proper citation: RRID:Addgene_55541 Copy
Species: Rattus norvegicus
Genetic Insert: G protein alpha-o with constitutively activating R179C mutation
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4800; Vector Backbone:pcDNAI/Amp; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17596375
Proper citation: RRID:Addgene_55780 Copy
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