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http://www.addgene.org/26428

Species: Mus musculus
Genetic Insert: partial sequence of mouse Titin
Vector Backbone Description: Backbone Size:7400; Vector Backbone:pRRL.SIN.cPPT.PGK.GFP; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_26428 Copy   

•   Source: Addgene
  

http://www.addgene.org/26421

Species: E.coli, HSV
Genetic Insert: HSV-TK, mCMV-b-gal
Vector Backbone Description: Backbone Size:3360; Vector Backbone:pBR322; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Ampicillin
Comments: There are some discrepancies between Addgene's quality control sequence and the author's sequence, but the Mitani lab has confirmed that this plasmid is correct. The depositor has also confirmed that there is an extra SacI site present in the plasmid is not apparent in the theoretical sequence on which the map below is based. Addgene does not recommend using SacI for a diagnostic digest.

Proper citation: RRID:Addgene_26421 Copy   

•   Source: Addgene
  

http://www.addgene.org/26387

Species: Caenorhabditis elegans
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20729841

Proper citation: RRID:Addgene_26387 Copy   

•   Source: Addgene
  

http://www.addgene.org/26381

Species: Homo sapiens
Genetic Insert: FUS
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:5299; Vector Backbone:pFRT_DESTFLAGHA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22081015

Proper citation: RRID:Addgene_26381 Copy   

•   Source: Addgene
  

http://www.addgene.org/26437

Species: unknown
Genetic Insert: bActin promoter
Vector Backbone Description: Backbone Size:4726; Vector Backbone:unknown; Vector Types:4726; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_26437 Copy   

•   Source: Addgene
  

http://www.addgene.org/26434

Genetic Insert: no insert
Vector Backbone Description: Backbone Size:9116; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Tet-On Advanced Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Need to be transduced into a Tet-On Advanced cell line for expression of the GOI. There are a few mismatches between Addgene's sequence and Dr Campeau's sequence. The sequence differences do not affect plasmid function.

Proper citation: RRID:Addgene_26434 Copy   

•   Source: Addgene
  

http://www.addgene.org/26436

Species: Danio rerio
Genetic Insert: flk1 promoter
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Topo cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16251212
Comments: From the paper: Approximately 6.5 kb of upstream sequence of the zebrafish flk1 gene (Liao et al., 1997; Thompson et al., 1998) was amplified from wild-type genomic DNA based on the sequence information previously deposited (from –6410 to –3 of the transcriptional start site, GenBank Accession Number AY045466) (Chan et al., 2002), and subcloned into the pCRII TOPO vector (Invitrogen). This fragment has been shown to drive endothelial-specific expression of the green-red coral fluorescent protein (G-RCFP) (Cross et al., 2003). The EcoRI cleaved fragment from this construct was placed 5′ upstream of the enhanced green fluorescent protein (EGFP) vector (Clontech) to drive endothelial specific expression of EGFP

Proper citation: RRID:Addgene_26436 Copy   

•   Source: Addgene
  

http://www.addgene.org/26435

Species: Mus musculus
Genetic Insert: cytoplasmic dynein 1 intermediate chain 1 isoform B
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4-TOPO; Vector Types:subcloning and sequencing; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20657784

Proper citation: RRID:Addgene_26435 Copy   

•   Source: Addgene
  

http://www.addgene.org/26430

Genetic Insert: no insert
Vector Backbone Description: Backbone Size:9402; Vector Backbone:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; Vector Types:Mammalian Expression, Lentiviral, Tet-On Advanced Destination vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Need to be transduced in a Tet-On advanced cell line to obtain expression of the GOI.

Proper citation: RRID:Addgene_26430 Copy   

•   Source: Addgene
  

http://www.addgene.org/26396

Genetic Insert: Cerulean-32-Venus
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:3937; Vector Backbone:EGFP N1; Vector Types:Mammalian Expression, FRET standard; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17040988
Comments: This construct contains Cerulean attached via a 32 amino acid linker to Venus. See supplemental methods section in associated paper for further details of plasmid construction.

Proper citation: RRID:Addgene_26396 Copy   

•   Source: Addgene
  

http://www.addgene.org/26398

Species: Homo sapiens
Genetic Insert: SOD1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20221404
Comments: The complete cDNA insert was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 4 of the cDNA from GCC to GTC, resulting in the A4V mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and XhoI sites on the 5’ and 3’ end respectively. This EcoRI-Xho1 0.47kb fragment was cloned into the respective EcoRI and XhoI sites of pcDNA3.1(+).

Proper citation: RRID:Addgene_26398 Copy   

•   Source: Addgene
  

http://www.addgene.org/26409

Species: Homo sapiens
Genetic Insert: SOD1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20221404
Comments: The complete cDNA was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 37 of the cDNA from GGA to AGA, resulting in the G37R mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and SalI sites on the 5’ and 3’ end respectively. This EcoRI-Sal1 0.47kb fragment was cloned into the respective EcoRI and SalI sites of pAcGFP1N1. Thus SOD1 is 5’ of GFP and SOD1 lies upstream of GFP in the SOD1-GFP fusion protein.

Proper citation: RRID:Addgene_26409 Copy   

•   Source: Addgene
  

http://www.addgene.org/26408

Species: Homo sapiens
Genetic Insert: SOD1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20221404
Comments: The complete cDNA was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 4 of the cDNA from GCC to GTC, resulting in the A4V mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and SalI sites on the 5’ and 3’ end respectively. This EcoRI-Sal1 0.47kb fragment was cloned into the respective EcoRI and SalI sites of pAcGFP1N1. Thus SOD1 is 5’ of GFP and SOD1 lies upstream of GFP in the SOD1-GFP fusion protein.

Proper citation: RRID:Addgene_26408 Copy   

•   Source: Addgene
  

http://www.addgene.org/26407

Species: Homo sapiens
Genetic Insert: SOD1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20221404
Comments: The complete cDNA was subcloned into the vector pET28 and then a PCR amplified the complete cDNA with EcoRI and SalI sites on the 5’ and 3’ end respectively. This EcoRI-Sal1 0.47kb fragment was cloned into the respective EcoRI and SalI sites of pAcGFP1N1. Thus SOD1 is 5’ of GFP and SOD1 lies upstream of GFP in the SOD1-GFP fusion protein.

Proper citation: RRID:Addgene_26407 Copy   

•   Source: Addgene
  

http://www.addgene.org/26368

Species: Homo sapiens
Genetic Insert: TAF15
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2278; Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21344536

Proper citation: RRID:Addgene_26368 Copy   

•   Source: Addgene
  

http://www.addgene.org/26367

Species: Homo sapiens
Genetic Insert: EWSR1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2278; Vector Backbone:pENTR4; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21344536

Proper citation: RRID:Addgene_26367 Copy   

•   Source: Addgene
  

http://www.addgene.org/26400

Species: Homo sapiens
Genetic Insert: SOD1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20221404
Comments: The complete cDNA insert was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 85 of the cDNA from GGC to CGC, resulting in the G85R mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and XhoI sites on the 5’ and 3’ end respectively. This EcoRI-Xho1 0.47kb fragment was cloned into the respective EcoRI and XhoI sites of pcDNA3.1(+).

Proper citation: RRID:Addgene_26400 Copy   

•   Source: Addgene
  

http://www.addgene.org/26403

Species: Homo sapiens
Genetic Insert: SOD1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20221404
Comments: The complete cDNA was subcloned into the vector pET28. Site directed mutagenesis changed wildtype DNA sequence for codon 4 of the cDNA from GCC to GTC, resulting in the A4V mutation in the protein and then a PCR amplified the complete cDNA with EcoRI and SalI sites on the 5’ and 3’ end respectively. This EcoRI-Sal1 0.47kb fragment was cloned into the respective EcoRI and SalI sites of pACGFPC1. Thus GFP is 5’ of the SOD1 insert and upstream in the GFP-SOD1 fusion protein.

Proper citation: RRID:Addgene_26403 Copy   

•   Source: Addgene
  

http://www.addgene.org/26402

Species: Homo sapiens
Genetic Insert: SOD1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20221404
Comments: The complete cDNA was subcloned into the vector pET28 and then a PCR amplified the complete cDNA with EcoRI and SalI sites on the 5’ and 3’ end respectively. This EcoRI-SalI 0.47kb fragment was cloned into the respective EcoRI and SalI sites of pACGFPC1. Thus GFP is 5’ of the SOD1 insert and upstream in the GFP-SOD1 fusion protein.

Proper citation: RRID:Addgene_26402 Copy   

•   Source: Addgene
  

http://www.addgene.org/26363

Genetic Insert: FLAG/HA
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3729; Vector Backbone:pET23(a); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21344536

Proper citation: RRID:Addgene_26363 Copy   

•   Source: Addgene