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Species: Caenorhabditis elegans
Genetic Insert: GFP:H2B from pKS111-His in pDONR201
Vector Backbone Description: Backbone Size:2257; Vector Backbone:pDONR201; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_17248 Copy
Species: Caenorhabditis elegans
Genetic Insert: L3787 promoter NO ATG in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20169625
Proper citation: RRID:Addgene_17251 Copy
Species: Caenorhabditis elegans
Genetic Insert: L3788 promoter NO ATG in pDONRP4P1R
Vector Backbone Description: Backbone Size:2645; Vector Backbone:pDONRP4P1R; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20169625
Proper citation: RRID:Addgene_17252 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-54 3' from pPD49.78 in pDONRP2RP3
Vector Backbone Description: Backbone Size:2641; Vector Backbone:pDONRP2RP3; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18818082
Proper citation: RRID:Addgene_17253 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-3::mScarlet
Vector Backbone Description: Backbone Size:2445; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37396790
Comments: C. elegans codon-optimized mScarlet. Use as a co-injection marker. pGLOW31 mScarlet fluorescence is brighter than pCFJ104 mCherry fluorescence in body wall muscle. Inject at 5 ng/uL. Made by George Huang (Glow Worms '20).
Proper citation: RRID:Addgene_172698 Copy
Species: Caenorhabditis elegans
Genetic Insert: Punc-25
Vector Backbone Description: Vector Backbone:Gateway final clone; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28767038
Comments: Please note that the Addgene NGS sequence differs from the depositor's annotated sequence. These differences do not affect plasmid function.
Proper citation: RRID:Addgene_172695 Copy
Species: Caenorhabditis elegans
Genetic Insert: mScarlet-I-C1^SEC^3xMyc
Vector Backbone Description: Backbone Size:2661; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37396790
Comments: C. elegans codon-optimized mScarlet. pGLOW39 is a derivative of published TagRFP-SEC vectors (Dickinson et al. Genetics 2015). It has a 3xMyc tag in place of 3xFlag and Lox2272 sites in place of LoxP. These features allow pGLOW39 to be used in a genetic background that has already been modified using a green FP-SEC vector, without conflicts between epitope tags and Lox sites. Made by George Huang (Glow Worms '20).
Proper citation: RRID:Addgene_173068 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::CIBN-3xFLAG::MP::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173744 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::mKate2::CRY2(olig)::VHH(GFP)::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173745 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::CRY2(olig)::VHH(mCherry)::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173747 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pwrt-2::CRY2(olig)::VHH(GFP)::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173748 Copy
Species: Caenorhabditis elegans
Genetic Insert: wrt-2 promoter
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:2675; Vector Backbone:pHSG298; Vector Types:Other; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34849800
Comments: Insert may be inserted in reverse orientation in the backbone plasmid. Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173741 Copy
Species: Caenorhabditis elegans
Genetic Insert: unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:2675; Vector Backbone:pHSG298; Vector Types:Other; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34849800
Comments: Insert may be inserted in reverse orientation in the backbone plasmid. Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173738 Copy
Species: Caenorhabditis elegans
Genetic Insert: rps-0 promoter
Vector Backbone Description: Backbone Marker:Takara Bio; Backbone Size:2675; Vector Backbone:pHSG298; Vector Types:Other; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34849800
Comments: Insert may be inserted in reverse orientation in the backbone plasmid. Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173730 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173753 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::HygR::unc-54 3'UTR; Psqt-1::sqt-1::sqt-1 3' UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4762; Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173755 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::mKate2::par-6::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173751 Copy
Species: Caenorhabditis elegans
Genetic Insert: Prps-0::mKate2:: par-6(_PB1)::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173752 Copy
Species: Caenorhabditis elegans
Genetic Insert: grh-1
Vector Backbone Description: Vector Backbone:pdestMB14 (Addgene #26415); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35013237
Proper citation: RRID:Addgene_177762 Copy
Species: Caenorhabditis elegans
Genetic Insert: wrmScarlet11^SEC^3xMyc
Vector Backbone Description: Backbone Size:3881; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Comments: The split-wrmScarlet system was developed and published by Goudeau et al. Genetics 2021. pGLOW63 is a derivative of published TagRFP-SEC vectors (Dickinson et al. Genetics 2015). It has a 3xMyc tag in place of 3xFlag and Lox2272 sites in place of LoxP. These features allow pGLOW63 to be used in a genetic background that has already been modified using a green FP-SEC vector, without conflicts between epitope tags and Lox sites. pGLOW63 must also be used in a genetic background that has wrmScarlet1-10. Made by Amelie Perez (Glow Worms '21).
Proper citation: RRID:Addgene_178877 Copy
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