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Vector Backbone Description: Vector Backbone:pUC; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:40645610
Proper citation: RRID:Addgene_229736 Copy
Species: Escherichia coli K-12 W3110
Genetic Insert: E.coli Type I-E CRISPR cas
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:2300; Vector Backbone:pCDF; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Streptomycin
Defining Citation: PMID:41290661
Proper citation: RRID:Addgene_247069 Copy
Species: Anthoceros agrestis
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Removal of Anthoceros agrestis RbcX2 and RbcX1 from pCDF_AaRaf1/Raf2/RbcX2/BSD2/RbcX1 was performed by Genscript.
Proper citation: RRID:Addgene_229515 Copy
Species: Arabidopsis thaliana
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Gene Synthesis from Genscript. Design of the Rubisco assembly factor inserts was mirroring pAtR1/R2/RX/B2 as shown in Aigner et al 2017 (10.1126/science.aap9221), with the addition of RbcX isoform, RbcXI after the BSD2 gene.
Proper citation: RRID:Addgene_229510 Copy
Species: Arabidopsis thaliana
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Gene Synthesis from Genscript. Design of the three Rubisco assembly factor inserts was mirroring pAtR1/R2/RX/B2 as shown in Aigner et al 2017 (10.1126/science.aap9221).
Proper citation: RRID:Addgene_229511 Copy
Species: Anthoceros agrestis
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Removal of Anthoceros agrestis Raf2 from pCDF_AaRaf1/Raf2/RbcX2/BSD2/RbcX1 was performed by Genscript.
Proper citation: RRID:Addgene_229513 Copy
Species: Anthoceros agrestis
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Fragments of assembly factors , with T7 promoter and terminator, with type II restriction cut sites at both ends were ordered from Twist Bioscience, construct was formed with a golden gate pot reaction to an receiver pCDF backbone.
Proper citation: RRID:Addgene_229507 Copy
Species: Anthoceros agrestis
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Gene Synthesis from Genscript. Each assembly factor is regulated by a individual T7 promoter and terminator.
Proper citation: RRID:Addgene_229509 Copy
Species: Anthoceros agrestis
Genetic Insert: Rubisco accumulation factor 1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39491367
Comments: Fragments of assembly factors , with T7 promoter and terminator, with type II restriction cut sites at both ends were ordered from Twist Bioscience, construct was formed with a golden gate pot reaction to an receiver pCDF backbone.
Proper citation: RRID:Addgene_229508 Copy
Vector Backbone Description: Backbone Size:5261; Vector Backbone:CloDF13; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39126322
Proper citation: RRID:Addgene_213132 Copy
Species: Synthetic
Genetic Insert: T0 terminator - Sm resistance cassette
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pSH624-ssr; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:37798358
Proper citation: RRID:Addgene_198026 Copy
Species: E. coli
Genetic Insert: PPhlF-PBetI-YFP
Vector Backbone Description: Backbone Marker:Alec Nielsen; Backbone Size:3359; Vector Backbone:pAN4020; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:27034378
Proper citation: RRID:Addgene_74698 Copy
Genetic Insert: n/a
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Streptomycin
Comments: This strain has 572 genetic changes compared to the DH10B reference genome in Genbank (CP000948.1). Please see https://www.ncbi.nlm.nih.gov/nuccore/CP110017 and the accompanying paper for more details.
Proper citation: RRID:Addgene_201049 Copy
Vector Backbone Description: Backbone Size:5007; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39244484
Comments: Low copy plasmid (pSC101 ori and repA), pUC19 MCS, confers streptomycin resistance
Proper citation: RRID:Addgene_225174 Copy
Vector Backbone Description: Backbone Size:4769; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39244484
Comments: Low copy plasmid (pSC101 ori and repA), pUC18 MCS with FLAG epitope integrated at SmaI site, confers streptomycin resistance
Proper citation: RRID:Addgene_225165 Copy
Vector Backbone Description: Backbone Size:4769; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39244484
Comments: Low copy plasmid (pSC101 ori and repA), pUC19 MCS with FLAG epitope integrated at SmaI site, confers streptomycin resistance
Proper citation: RRID:Addgene_225164 Copy
Species: E. coli (Bacteria)
Genetic Insert: potH
Vector Backbone Description: Vector Backbone:PCDF Bravo ; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:40154612
Comments: IPTG inducible
Proper citation: RRID:Addgene_216749 Copy
Species: E. coli (Bacteria)
Genetic Insert: potH
Vector Backbone Description: Vector Backbone:PCDF Bravo ; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:40154612
Comments: IPTG inducible
Proper citation: RRID:Addgene_216750 Copy
Species: E. coli (Bacteria)
Genetic Insert: potH
Vector Backbone Description: Vector Backbone:PCDF Bravo ; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:40154612
Comments: IPTG inducible
Proper citation: RRID:Addgene_216752 Copy
Species: E. coli (Bacteria)
Genetic Insert: potH
Vector Backbone Description: Vector Backbone:PCDF Bravo ; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:40154612
Comments: IPTG inducible
Proper citation: RRID:Addgene_216751 Copy
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