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Species: Rattus norvegicus
Genetic Insert: BE3-2XNLS
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32978399
Proper citation: RRID:Addgene_135348 Copy
Species: Rattus norvegicus
Genetic Insert: Munc18b
Vector Backbone Description: Vector Backbone:pFastBac; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30317509
Proper citation: RRID:Addgene_135554 Copy
Species: Rattus norvegicus
Genetic Insert: miR-30 rat SST
Vector Backbone Description: Vector Backbone:pAAV SYN1 iRFP-FLAG; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32205443
Proper citation: RRID:Addgene_135565 Copy
Species: Rattus norvegicus
Genetic Insert: Rat liver fatty acid binding protein
Vector Backbone Description: Backbone Marker:Pharmacia; Backbone Size:4000; Vector Backbone:pTrc99; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15914028
Proper citation: RRID:Addgene_13577 Copy
Species: Rattus norvegicus
Genetic Insert: Rat Fibronectin
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2869; Vector Backbone:pGEM-2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:6317187
Proper citation: RRID:Addgene_14057 Copy
Species: Rattus norvegicus
Genetic Insert: Zdk2, Cofilin, LOV2
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31740825
Comments: There is a point mutation in the Zdk portion of the dark control construct. In our hands, this had no effect on the experiments, and it is a position where it would not be expected to impact binding or optogenetic response.
Proper citation: RRID:Addgene_141135 Copy
Species: Rattus norvegicus
Genetic Insert: OMP25
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4692; Vector Backbone:pAcGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32328629
Proper citation: RRID:Addgene_141150 Copy
Species: Rattus norvegicus
Genetic Insert: OMP25
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4689; Vector Backbone:pAcGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32328629
Proper citation: RRID:Addgene_141151 Copy
Species: Rattus norvegicus
Genetic Insert: TGF beta type I receptor
Vector Backbone Description: Backbone Marker:Genentech; Backbone Size:4700; Vector Backbone:pRK5 modified with flag; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8662796
Comments: See Author's map for more information.
A 9bp linker was added directly upstream of the GS domain. The linker encodes the amino acids GSM. The additional sequence does not affect plasmid function.
Proper citation: RRID:Addgene_14833 Copy
Species: Rattus norvegicus
Genetic Insert: YFP-PKCbetaII-YFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12782683
Comments: YFP-PKCbetaII-YFP was made by PCR of mYFP from HindII to KpnI, rat PKCbetaII from KpnI to XhoI, and YFP from XhoI to XbaI.
Proper citation: RRID:Addgene_14866 Copy
Species: Rattus norvegicus
Genetic Insert: GLUT2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8626492
Comments: cDNA clone isolated from a rat islet cDNA library constructed in pcDNA-1. The full length insert was excised by KpnI/XbaI and subcloned into pcDNA3.
Proper citation: RRID:Addgene_14946 Copy
Species: Rattus norvegicus
Genetic Insert: Rat insulin promoter (RIP)
Vector Backbone Description: Backbone Size:6700; Vector Backbone:modified pBluescript II KS; Vector Types:Mammalian Expression, Cre/Lox, for making transgenic constructs; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15129273
Comments: The CRE-ER(TM) protein requires tamoxifen (TM) to catalyze LoxP site-mediated excision.
The RIP–CreER construct was generated by fusing a 0.66-kilobase SmaI–HindIII fragment of the RIP2 promoter to a minimal hsp68 promoter, and placing the chimaeric promoter upstream of CreER(TM).
See Author's Map for more information.
Proper citation: RRID:Addgene_15029 Copy
Species: Rattus norvegicus
Genetic Insert: JNK3
Vector Backbone Description: Backbone Marker:Bruce Mayer Lab; Backbone Size:6000; Vector Backbone:pEBG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_15005 Copy
Species: Rattus norvegicus
Genetic Insert: Hes1
Vector Backbone Description: Backbone Marker:Gary Nolan, Stanford; Backbone Size:0; Vector Backbone:pBABE-MN-IRES-E-GFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10839357
Comments: Dominant negative Hes1.
Please note that the vector backbone pBABE-MN-IRES-E-GFP (also known as pBMN-I-GFP, Addgene plasmid# 1736) is extremely slow growing and may need 2 days to grow.
Proper citation: RRID:Addgene_15134 Copy
Species: Rattus norvegicus
Genetic Insert: insulin II promoter
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4100; Vector Backbone:pTimer-1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15129273
Comments: See Author's Map for more information.
Proper citation: RRID:Addgene_15109 Copy
Species: Rattus norvegicus
Genetic Insert: Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
Vector Backbone Description: Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32483365
Proper citation: RRID:Addgene_139106 Copy
Species: Rattus norvegicus
Genetic Insert: gRNA and GFP donor
Vector Backbone Description: Backbone Size:9667; Vector Backbone:pORANGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32275651
Proper citation: RRID:Addgene_139659 Copy
Species: Rattus norvegicus
Genetic Insert: gRNA and GFP donor
Vector Backbone Description: Backbone Size:9263; Vector Backbone:pORANGE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32275651
Proper citation: RRID:Addgene_139662 Copy
Species: Rattus norvegicus
Genetic Insert: AMPK-Kinase-dead
Vector Backbone Description: Vector Backbone:pLenti-X1-Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32160526
Proper citation: RRID:Addgene_139844 Copy
Species: Rattus norvegicus
Genetic Insert: BE4max(R33A)_NG-ΔUGI
Vector Backbone Description: Backbone Marker:pCMV-ABEmax-P2A-EGFP (Addgene #112101); Backbone Size:3400; Vector Backbone:CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32690971
Proper citation: RRID:Addgene_140257 Copy
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