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Species: Synthetic
Genetic Insert: OmpA_H-78
Vector Backbone Description: Backbone Marker:DNA2.0; Backbone Size:4000; Vector Backbone:pJexpress414; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26199411
Comments: Amino acid sequence: mkktaiaiavalagfatvaqaapndntWyTgAkLgWsqyhdtgfinnngpthenqLgAgAfGgYqVnpyVgFeMgYdWlgrmpykgsvengaykaqgVqLtAkLgYpitddLdIyTrLgGmVwradtksnvygknhdtgvsPvFaGgVeYaItpeIaMrLeTqHtnnigdYPYDVPDYAtrpdnWmVsLgAsYrfgCCPGCCHHHHHH
Proper citation: RRID:Addgene_64313 Copy
Species: Synthetic
Genetic Insert: Tobacco etch virus (TEV) protease
Vector Backbone Description: Backbone Size:5440; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25733847
Proper citation: RRID:Addgene_64276 Copy
Species: Synthetic
Genetic Insert: LifeAct
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES Hyg3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_64343 Copy
Species: Synthetic
Genetic Insert: Citrine-ADH1-tail
Vector Backbone Description: Backbone Marker:Robert Gnuegge; Backbone Size:4557; Vector Backbone:pRG216; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26647923
Proper citation: RRID:Addgene_64510 Copy
Species: Synthetic
Genetic Insert: LexA-ER-B42
Vector Backbone Description: Backbone Marker:Robert Gnuegge; Backbone Size:4646; Vector Backbone:pRG205; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26647923
Proper citation: RRID:Addgene_64537 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Scott Sternson; Backbone Size:5088; Vector Backbone:AAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: The plasmid is fully sequenced in the coding sequence regions (fluorophore and important flanking regions). Multiple digestions were done to verify the vector structure. The construct and the virus were both tested in vitro.
In addition, the published CaMKII promoter contains 10 basepair differences vs. the wild-type, and that persists here.
Proper citation: RRID:Addgene_64545 Copy
Species: Synthetic
Genetic Insert: 12x PP7 stem-loops
Vector Backbone Description: Vector Backbone:pSF4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25792328
Proper citation: RRID:Addgene_64544 Copy
Species: Synthetic
Genetic Insert: Linker for joining modules with 3' TTCG overhangs to modules with 5' GTGG overhangs
Vector Backbone Description: Vector Backbone:Lab made; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50347 Copy
Species: Synthetic
Genetic Insert: Linker for joining modules with 3' GTGG overhangs to modules with 5' TTCG overhangs
Vector Backbone Description: Vector Backbone:Lab made; Vector Types:plant expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24933124
Comments: insert can be released with BsaI
Proper citation: RRID:Addgene_50346 Copy
Species: Synthetic
Genetic Insert: SNAP
Vector Backbone Description: Backbone Size:5388; Vector Backbone:pCDNA3.1(+)-myc-GP135; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23615438
Proper citation: RRID:Addgene_50376 Copy
Species: Synthetic
Genetic Insert: CLIP
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pME-puro-FLAG-CD59; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23615438
Proper citation: RRID:Addgene_50375 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pME-FLAG-CD59-GPI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20477992
Proper citation: RRID:Addgene_50378 Copy
Species: Synthetic
Genetic Insert: Venus
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pME-puro-FLAG-CD59; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20477992
Comments: Please click View Sequences link for full plasmid sequence of vector backbone pME-puro-FLAG-CD59.
Proper citation: RRID:Addgene_50377 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Registry of Standard Biological Parts; Backbone Size:3296; Vector Backbone:pSB4A3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24608181
Comments: Note: Addgene's quality control sequencing found a few mismatches compared to the depositor's full sequence, but they should not affect plasmid function.
Proper citation: RRID:Addgene_50549 Copy
Species: Synthetic
Genetic Insert: Cph8
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1995; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24608181
Comments: Encodes the Cph8 (Cph1/EnvZ fusion) light sensor kinase/phosphatase downstream of the pLTetO-1 promoter.
Requires pPLPCB(S)t or some other source of TetR
Requires PCB to function
Does NOT include the response regulator.
Proper citation: RRID:Addgene_50552 Copy
Species: Synthetic
Genetic Insert: Luciferase-let7-Poly(A)
Vector Backbone Description: Backbone Marker:The Drosophila Gateway™ Vector Collection; Vector Backbone:pAHW; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23123195
Comments: This plasmid expresses a Renilla luciferase reporter for let-7 miRNA with a regular polyadenylation signal.
Proper citation: RRID:Addgene_50556 Copy
Species: Synthetic
Genetic Insert: DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
Vector Backbone Description: Backbone Size:6400; Vector Backbone:pRS426 Gal1; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11000112
Comments: There is a H to T mutation in front of the MetBetaGal. The mutation should not affect function.
Proper citation: RRID:Addgene_50572 Copy
Species: Synthetic
Genetic Insert: zCas9D10A
Vector Backbone Description: Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50582 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB
Vector Backbone Description: Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50580 Copy
Species: Synthetic
Genetic Insert: dCas9-KRAB
Vector Backbone Description: Backbone Marker:Mullineaux Lab, see http://www.pgreen.ac.uk/; Vector Backbone:pGreen-like binary vector; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Kanamycin and Spectinomycin
Defining Citation: PMID:25432517
Proper citation: RRID:Addgene_50587 Copy
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