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Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2807; Vector Backbone:hSTARR-seq_mCP vector; Vector Types:Mammalian Expression, hSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29256496
Comments: This screening vector can screen a DNA library which can be inserted via the AgeI and SalI restriction sides using subsequent In-Fusion reaction (Clontech) Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
Proper citation: RRID:Addgene_99291 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2857; Vector Backbone:hSTARR-seq_TTF2 vector; Vector Types:Mammalian Expression, hSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29256496
Comments: This screening vector can screen a DNA library which can be inserted via the AgeI and SalI restriction sides using subsequent In-Fusion reaction (Clontech) . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
Proper citation: RRID:Addgene_99293 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2857; Vector Backbone:hSTARR-seq_OCT4 3UTR vector; Vector Types:Mammalian Expression, hSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29256496
Comments: This screening vector can screen a DNA library which can be inserted via the AgeI and SalI restriction sides using subsequent In-Fusion reaction (Clontech) . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
Proper citation: RRID:Addgene_99295 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26850639
Proper citation: RRID:Addgene_99911 Copy
Vector Backbone Description: Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26850639
Proper citation: RRID:Addgene_99912 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster zfh1 enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28024147
Comments: to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)
Proper citation: RRID:Addgene_86381 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster ssp3 enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28024147
Comments: to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)
Proper citation: RRID:Addgene_86382 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3477; Vector Backbone:pGL3; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28024147
Comments: to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)
Proper citation: RRID:Addgene_86380 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster tj enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28024147
Comments: to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)
Proper citation: RRID:Addgene_86386 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster ham enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28024147
Comments: to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)
Proper citation: RRID:Addgene_86383 Copy
Species: Drosophila melanogaster
Genetic Insert: D. melanogaster sgl enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3426; Vector Backbone:pGL3; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28024147
Comments: to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)
Proper citation: RRID:Addgene_86384 Copy
Vector Backbone Description: Backbone Size:8793; Vector Backbone:pNOC-Flux; Vector Types:Algae, Nannochloropsis; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30883973
Proper citation: RRID:Addgene_98141 Copy
Vector Backbone Description: Backbone Size:9947; Vector Backbone:pSELECT100; Vector Types:Algae, Nannochloropsis; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30883973
Proper citation: RRID:Addgene_98140 Copy
Vector Backbone Description: Backbone Size:10996; Vector Backbone:pNOC-Flux; Vector Types:Algae, Nannochloropsis; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30883973
Proper citation: RRID:Addgene_98143 Copy
Genetic Insert: MevTo-BBa1002-pTrc-Mkco-aphA
Vector Backbone Description: Backbone Size:3288; Vector Backbone:p15A and ColE1; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26708516
Proper citation: RRID:Addgene_81013 Copy
Genetic Insert: MevTo-BBa1002-pTrc-Mkco-PMKco
Vector Backbone Description: Backbone Size:3288; Vector Backbone:p15A and ColE1; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26708516
Proper citation: RRID:Addgene_81016 Copy
Genetic Insert: MevTo-BBa1002-pTrc-Mkco
Vector Backbone Description: Backbone Size:3288; Vector Backbone:p15A and ColE1; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:26708516
Proper citation: RRID:Addgene_81017 Copy
Vector Backbone Description: Backbone Size:7522; Vector Backbone:pKW32; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pWK32 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate an expression clone containing a hemoglobin tag.
Proper citation: RRID:Addgene_18669 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7694; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pcDNA3 Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction to generate a mammalian expression clone.
Proper citation: RRID:Addgene_18672 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:11282; Vector Backbone:pPIC3.5K; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18064608
Comments: pPIC3.5K Gateway-compatible Destination vector. Combine with an Entry clone containing your gene of interest with the Gateway LR reaction for integration of gene of interest into Pichia.
Proper citation: RRID:Addgene_18707 Copy
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