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Genetic Insert: CreER
Vector Backbone Description: Backbone Size:3392; Vector Backbone:pT3; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28009940
Proper citation: RRID:Addgene_85577 Copy
Genetic Insert: CreER
Vector Backbone Description: Backbone Size:3392; Vector Backbone:pT3; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28009940
Proper citation: RRID:Addgene_85578 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85723 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85720 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85724 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85728 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85729 Copy
Vector Backbone Description: Vector Backbone:pCS2+ F/A; Vector Types:mammalian/avian/xenopus/zebrafish; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27797719
Proper citation: RRID:Addgene_85719 Copy
Vector Backbone Description: Backbone Size:9127; Vector Backbone:LentiGuide; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27458201
Proper citation: RRID:Addgene_85745 Copy
Vector Backbone Description: Backbone Marker:Rubin Lab; Backbone Size:10726; Vector Backbone:pBPGw; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28245922
Comments: VanGlow-RR-promoter is a Gateway compatible vector enabling high throughput cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter protein (codon optimized for expression in Drosophila) using Invitrogen LR clonase. This vector can be used for Renilla-luciferase assays in S2 cells and can be inserted into specific target sites in the Drosophila genome using PhiC31 integrase and used to assess reporter gene expression in vivo using Immunofluorescence, Live-Cell-Imaging, and Renilla-luciferase assays. In addition, this vector may be used in combination with other VanGlow transgenes to facilitate FACS purification of specific populations of cells.
Proper citation: RRID:Addgene_83343 Copy
Vector Backbone Description: Backbone Marker:Rubin Lab; Backbone Size:11536; Vector Backbone:pBPGUw; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28245922
Comments: VanGlow-GL-enhancer is a Gateway compatible vector enabling high throughput cloning of enhancer elements upstream of the Drosophila synthetic core promoter (DSCP) and a GFP::Luciferase(nls) reporter protein (codon optimized for expression in Drosophila) using Invitrogen LR clonase. This vector can be used for Luciferase assays in S2 cells and can be inserted into specific target sites in the Drosophila genome using PhiC31 integrase, and used to assess reporter gene expression in vivo using Immunofluorescence, Live-Cell-Imaging, and Luciferase assays. In addition, this vector may be used in combination with other VanGlow transgenes to facilitate FACS purification of specific populations of cells.
Proper citation: RRID:Addgene_83338 Copy
Vector Backbone Description: Backbone Marker:Rubin Lab; Backbone Size:10876; Vector Backbone:pBPGUw; Vector Types:Insect Expression, Luciferase; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28245922
Comments: VanGlow-RR-enhancer is a Gateway compatible vector enabling high throughput cloning of enhancer elements upstream of the Drosophila synthetic core promoter (DSCP) and a mCherry::Renilla(nls) reporter protein (codon optimized for expression in Drosophila) using Invitrogen LR clonase. This vector can be used for Renilla-luciferase assays in S2 cells and can be inserted into specific target sites in the Drosophila genome using PhiC31 integrase and used to assess reporter gene expression in vivo using Immunofluorescence, Live-Cell-Imaging, and Renilla-luciferase assays. In addition, this vector may be used in combination with other VanGlow transgenes to facilitate FACS purification of specific populations of cells.
Proper citation: RRID:Addgene_83339 Copy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24979769
Proper citation: RRID:Addgene_83877 Copy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_83879 Copy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24979769
Proper citation: RRID:Addgene_83878 Copy
Vector Backbone Description: Backbone Marker:David Root Lab (addgene #41390); Backbone Size:9621; Vector Backbone:pLEX_305; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29581585
Proper citation: RRID:Addgene_91798 Copy
Species: Phaeodactylum tricornutum
Genetic Insert: TALEN Backbone
Vector Backbone Description: Backbone Marker:GenBank_NCBI: JN180663; Backbone Size:3860; Vector Backbone:pPha-NR; Vector Types:Phaeodactylum tricornutum; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: N.E. Sanjana, L. Cong, Y. Zhou, M.M. Cunniff, G. Feng, F. Zhang
A transcription activator-like effector toolbox for genome engineering
Nat. Protoc., 7 (2012), pp. 171–192
Proper citation: RRID:Addgene_90421 Copy
Species: Phaeodactylum tricornutum
Genetic Insert: TALEN Backbone
Vector Backbone Description: Backbone Marker:GenBank_NCBI: JN180663; Backbone Size:3860; Vector Backbone:pPha-NR; Vector Types:Phaeodactylum tricornutum; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: N.E. Sanjana, L. Cong, Y. Zhou, M.M. Cunniff, G. Feng, F. Zhang
A transcription activator-like effector toolbox for genome engineering
Nat. Protoc., 7 (2012), pp. 171–192
Proper citation: RRID:Addgene_90423 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4342; Vector Backbone:STARR-seq luciferase INF enhancer vector_ORI; Vector Types:Mammalian Expression, hSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29256496
Comments: This vector has a bGH poly A sequence upstream of the ORI and a RBG polyA and a bGH poly A sequence downstream of the ORI; for blocking transcripts arising from the ORI; Core promoter is SCP1 . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
Proper citation: RRID:Addgene_99317 Copy
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4457; Vector Backbone:STARR-seq luciferase INF enhancer vector_ORI; Vector Types:Mammalian Expression, hSTARR-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29256496
Comments: This vector has a bcl 2 splice acceptor and 4x polyA sequences downstream of the ORI; for blocking transcripts arising from the ORI; Core promoter is SCP1 . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590
Proper citation: RRID:Addgene_99318 Copy
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