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http://www.addgene.org/63856

Species: Synthetic
Genetic Insert: No-aptamer control mRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26621913

Proper citation: RRID:Addgene_63856 Copy   

•   Source: Addgene

http://www.addgene.org/63857

Species: Synthetic
Genetic Insert: TMR7 TMR riboswitch
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26621913

Proper citation: RRID:Addgene_63857 Copy   

•   Source: Addgene

http://www.addgene.org/63858

Species: Synthetic
Genetic Insert: TMR10 TMR riboswitch
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26621913

Proper citation: RRID:Addgene_63858 Copy   

•   Source: Addgene

http://www.addgene.org/63852

Species: Synthetic
Genetic Insert: DNT3 DNT riboswitch
Vector Backbone Description: Vector Backbone:pFTV1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26621913

Proper citation: RRID:Addgene_63852 Copy   

•   Source: Addgene

http://www.addgene.org/63850

Species: Synthetic
Genetic Insert: Theo45 theophylline riboswitch
Vector Backbone Description: Vector Backbone:pFTV1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26621913

Proper citation: RRID:Addgene_63850 Copy   

•   Source: Addgene

http://www.addgene.org/63885

Species: Synthetic
Genetic Insert: ER-localized low-affinity GCaMP3(10.19)
Vector Backbone Description: Backbone Marker:OTTC/NIDA; Vector Backbone:pOTTC374 - pAAV EF1a DIO iRFP; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26451944

Proper citation: RRID:Addgene_63885 Copy   

•   Source: Addgene

http://www.addgene.org/63890

Species: Synthetic
Genetic Insert: SS Stuffer
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pComb3H; Vector Types:Bacterial Expression, phage display; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10986398
Comments: pComb3X is the newest of the pComb vectors. Improvements over pComb3 include increased stability and introduction of an asymmetric SfiI cassette for directional cloning of full Fab, scFv, peptide and other protein for phage display. 6xHis and HA tags allow for purification and detection. An amber stop codon was introduced to turn-off expression of the pIII fusion protein by switching to a non-supressor strain of E. coli allowing production of soluble protein without subcloning. Alternatively, the gene for phage protein pIII can be removed by SpeI/NheI digest. pComb3XSS is recommended for preparation of vector for library cloning. The “SS” refers to the double stuffer, a 1200bp stuffer in the Fab light chain cloning region bounded by SacI and XbaI restriction sites and a 300bp stuffer in Fab heavy chain cloning region bound by XhoI and SpeI restriction sites. Also, the 1600bp double stuffer (both stuffers plus the leader sequence between the Fab light chain and heavy chain cloning regions) can be removed by SfiI digest so that non-Fab genes of interest can be cloned. Also available on Addgene: pComb3XTT and pComb3XLambda are only needed at templates for the construction of chimeric Fab libraries as described in Phage Display: A Laboratory Manual. pComb3XTT can also be used as an Fab expression control. Select References: Barbas, C. F., III; Burton, D. R.; Scott, J.K., Silverman, G.J. Eds. (2001) Phage Display: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York Rader, C; Popkov, M.; Neves, J.A.; Barbas III, C.F. (2002) Integrin _v_3 Targeted Therapy of Kaposi’s Sarcoma with an In Vitro Evolved Antibody. FASEB, 16(14):2000-2. Berry, J.D.; Rutherford, J.; Silverman, G.J.; Kaul, R.; Elia, M.; Gobuty, S.; Fuller, R.; Plummer, F.A.; & Barbas III, C.F. (2003) Development of Functional Human Monoclonal Single-Chain Variable Fragment Antibody Against HIV-1 From Human Cervical B cells. Hybridoma and Hybridomics, 22(2):, 97-108. Jendreyko N, Popkov M, Beerli RR, Chung J, McGavern DB, Rader C, Barbas CF 3rd. (2003) Intra-diabodies: Bispecific, tetravalent antibodies for the simultaneous functional knockout of two cell surface receptors. J Biol Chem., 278(48):, 47812-9. Steinberger, P.; Sutton, J.K.; Rader, C.; Elia, M.; and Barbas III, C. F. (2000) Generation and Characterization of a Recombinant Human CCR5-specific Antibody: A Phage Display Approach for Rabbit Antibody Humanization. J. Biol. Chem., 275,, 36073-36078. Goncalves, J.; Kilva, F.; Freitas-Vieira, A.; Santa-Marta, M.; Malho, R.; Yang, X.; Gabuzda, D.; and Barbas III, C.F. (2002) Functional Neutralization of HIV-1 Vif Protein by Intracellular Immunization Inhibits Reverse Transcription and Viral Replication. J. Biol. Chem., 277(35):32036-45. Popkov, M.; Mage, R.G.; Alexander, C.B.; Thundivalappil, S.; Barbas III, C.F.; Rader, C. (2003) Rabbit immune repertoires as sources for therapeutic monoclonal antibodies: The impact of Kappa Allotype-correlated variation in cysteine content on antibody libraries selected by phage display. J. Mol. Biol., 325:325-335. Chung, J.; Rader, C.; Popkov, M.; Hur, Y.-M.; Kim, H.-K.; Lee, Y.-J.; & Barbas III, C. F. (2004) Integrin _IIb_3 specific synthetic human monoclonal antibodies and HCDR3 peptides that potently inhibit platelet aggregation,FASEB 18(2):361-3. Popkov, M.; Jendreyko, N.; Gonzalez-Sapienza, G.; Mage, R.G.; Rader, C.; Barbas III, C.F. (2004) Human/mouse cross-reactive anti-VEGF receptor 2 recombinant antibodies selected from animmune b9 allotype rabbit antibody library, J. Immunol. Methods, 288(1-2):149-164. Popkov, M.; Rader, C.; Barbas III, C.F. (2004) Isolation of human prostate cancer reactive antibodies using phage display technology, J. Immunol. Methods, 291:137-151. Tanaka, F.; Fuller, R.; Barbas III, C.F. (2005) Development of Small Designer Aldolase Enzymes: Catalytic Activity, Folding, and Substrate Specificity. Biochemistry, 44:7583-7592. This plasmid has been found to be prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. See our article on Plasmid Dimers and Multimers for more information (https://blog.addgene.org/plasmids-101-dimers-and-multimers)

Proper citation: RRID:Addgene_63890 Copy   

•   Source: Addgene

http://www.addgene.org/64074

Species: Synthetic
Genetic Insert: O-acetyl-L-homoserine sulfhydrylase
Vector Backbone Description: Backbone Marker:DNA 2.0; Vector Backbone:pJ411; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25884979
Comments: For expression of non-tagged oah2 only.

Proper citation: RRID:Addgene_64074 Copy   

•   Source: Addgene

http://www.addgene.org/64161

Species: Synthetic
Genetic Insert: sgRNA1 for Tet-inducible Luciferase reporter
Vector Backbone Description: Backbone Marker:Charles Gersbach, Addgene plasmid 47108; Vector Backbone:pSPgRNA; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25619936

Proper citation: RRID:Addgene_64161 Copy   

•   Source: Addgene

http://www.addgene.org/64201

Species: Synthetic
Genetic Insert: Hypoxanthine guanine xanthine phosphoribosyltransferase
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDEST14; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22726686

Proper citation: RRID:Addgene_64201 Copy   

•   Source: Addgene

http://www.addgene.org/64109

Species: Synthetic
Genetic Insert: Nm dCas9
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHAGE-DEST; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713381

Proper citation: RRID:Addgene_64109 Copy   

•   Source: Addgene

http://www.addgene.org/64105

Species: Synthetic
Genetic Insert: Sp dCas9
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHAGE-DEST; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713381

Proper citation: RRID:Addgene_64105 Copy   

•   Source: Addgene

http://www.addgene.org/64102

Species: Synthetic
Genetic Insert: rTTA
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBR322; Vector Types:Mammalian Expression, Mouse Targeting, Tet Inducible; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24082130

Proper citation: RRID:Addgene_64102 Copy   

•   Source: Addgene

http://www.addgene.org/64101

Species: Synthetic
Genetic Insert: mTagBFP2
Vector Backbone Description: Backbone Size:13493; Vector Backbone:pVSV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Proper citation: RRID:Addgene_64101 Copy   

•   Source: Addgene

http://www.addgene.org/64119

Species: Synthetic
Genetic Insert: dCas9-trCIB1 fusion
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1/V5-His A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25619936

Proper citation: RRID:Addgene_64119 Copy   

•   Source: Addgene

http://www.addgene.org/64195

Species: Synthetic
Genetic Insert: ROZA-XL YF
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5445; Vector Backbone:pCDNA 3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25735979
Comments: Reference for CFP: Zacharias DA, Violin JD, Newton AC, Tsien RY (2002). Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296: 913-916. Reference for YPET, yellow fluorescent protein: A.W. Nguyen, P.S. Daugherty, Evolutionary optimization of fluorescent proteins for intracellular FRET, Nat. Biotechnol. 23 (2005) 355e360

Proper citation: RRID:Addgene_64195 Copy   

•   Source: Addgene

http://www.addgene.org/64194

Species: Synthetic
Genetic Insert: ROZA-XL
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5445; Vector Backbone:pCDNA 3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25735979
Comments: Reference for CFP: Zacharias DA, Violin JD, Newton AC, Tsien RY (2002). Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296: 913-916. Reference for YPET, yellow fluorescent protein: A.W. Nguyen, P.S. Daugherty, Evolutionary optimization of fluorescent proteins for intracellular FRET, Nat. Biotechnol. 23 (2005) 355e360

Proper citation: RRID:Addgene_64194 Copy   

•   Source: Addgene

http://www.addgene.org/64112

Species: Synthetic
Genetic Insert: St1 dCas9
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pHAGE-DEST; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713381

Proper citation: RRID:Addgene_64112 Copy   

•   Source: Addgene

http://www.addgene.org/64115

Species: Synthetic
Genetic Insert: Nm sgRNA1.1
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713381

Proper citation: RRID:Addgene_64115 Copy   

•   Source: Addgene

http://www.addgene.org/64114

Species: Synthetic
Genetic Insert: Sp sgRNA2
Vector Backbone Description: Backbone Size:8000; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25713381

Proper citation: RRID:Addgene_64114 Copy   

•   Source: Addgene