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Species: E. coli
Genetic Insert: PPK
Vector Backbone Description: Vector Backbone:p416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35862758
Proper citation: RRID:Addgene_183943 Copy
Species: E. coli
Genetic Insert: PPK
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35862758
Proper citation: RRID:Addgene_183941 Copy
Species: E. coli
Genetic Insert: 35S::nptII-T-Nos
Vector Backbone Description: Backbone Size:4377; Vector Backbone:n/a; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36995631
Proper citation: RRID:Addgene_191783 Copy
Species: E. coli
Genetic Insert: RNase III
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36493370
Proper citation: RRID:Addgene_189537 Copy
Species: E. coli
Genetic Insert: MG1655-C321 mutS+, λ-, Δ(ybhB-bioAB)::zeoR, ΔprfA, ΔserB
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:36280721
Comments: MG1655-C321 -- All 321 UAG codons changed to UAA.
mutS+ -- Restores normal spontaneous mutation frequency.
(λ-) – Deleted Lambda Phage operon to allow growth at 37C.
Δ(ybhB-bioAB)::zeoR – Deletion of biotin biosynthetic operon using Zeocin cassette; strain is d-biotin auxotroph.
ΔprfA – Deletion of polypeptide release factor 1 (RF1) to prevent translation termination at UAG codons.
ΔserB – Deletion of phosphoserine phosphatase to provide intracellular source of phosphoserine; strain is Serine auxotroph.
The genome sequence of a similar strain (C321.deltaA) was deposited at NCBI (accession # CP006698.1)
Proper citation: RRID:Addgene_192872 Copy
Species: E. coli
Genetic Insert: LacZalpha
Vector Backbone Description: Backbone Marker:Alexeyev lab; Backbone Size:3008; Vector Backbone:pMA3735; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35883613
Proper citation: RRID:Addgene_184848 Copy
Species: E. coli
Genetic Insert: anti rabbit secondary nanobody fused to Tn5
Vector Backbone Description: Vector Backbone:pET-29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36536148
Comments: Please visit https://doi.org/10.1101/2022.03.08.483459 for bioRxiv preprint.
Proper citation: RRID:Addgene_183638 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191620 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191621 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191627 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191625 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191629 Copy
Species: E. coli
Genetic Insert: reverse tetracycline-regulated transactivator advanced
Vector Backbone Description: Backbone Marker:unpublished; Backbone Size:7200; Vector Backbone:pMA2635rc; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19655272
Proper citation: RRID:Addgene_25435 Copy
Species: E. Coli
Genetic Insert: LplA-W37I
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20534555
Comments: The original E. coli LplA gene from John Cronan was resynthesized using codons optimal for mammalian expression.
Proper citation: RRID:Addgene_25846 Copy
Species: E. Coli
Genetic Insert: LplA-W37V
Vector Backbone Description: Backbone Size:5500; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20534555
Comments: The original E. coli LplA gene from John Cronan was resynthesized using codons optimal for mammalian expression.
Proper citation: RRID:Addgene_25845 Copy
Species: E. coli
Genetic Insert: beta-gal
Vector Backbone Description: Backbone Size:10500; Vector Backbone:pUB23; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3018930
Comments: Half life of beta-gal ~3 minutes. See author's map.
Proper citation: RRID:Addgene_8820 Copy
Species: E. coli
Genetic Insert: beta-gal
Vector Backbone Description: Backbone Size:10500; Vector Backbone:pUB23; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3018930
Comments: Half life of beta-gal ~3 minutes. See author's map.
Proper citation: RRID:Addgene_8819 Copy
Species: E. coli
Genetic Insert: beta-gal
Vector Backbone Description: Backbone Size:10500; Vector Backbone:pUB23; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3018930
Comments: Half life of beta-gal ~7 minutes. See author's map.
Proper citation: RRID:Addgene_8817 Copy
Species: E. Coli
Genetic Insert: tet operator
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10440378
Comments: the tet operator can be released from this plasmid using a XhoI digest.
The construct is composed of the tet operator sequences followed by a CMV minimal promoter. The insert was digested with XhoI and EcoRI, klenow filled, and cloned into the SmaI site of pBluescript KS. See author's map for more information.
Proper citation: RRID:Addgene_8901 Copy
Species: E. coli
Genetic Insert: YedK
Vector Backbone Description: Backbone Marker:Sigma; Backbone Size:5400; Vector Backbone:p3XFlag-EYFP (Addgene vector 34582).; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29020633
Proper citation: RRID:Addgene_84567 Copy
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