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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 helicase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725308.1. Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_153891 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: 43740574
Proper citation: RRID:Addgene_153897 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725303.1
Proper citation: RRID:Addgene_153896 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR
Vector Backbone Description: Backbone Size:1968; Vector Backbone:pG9m-2 backbone; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: This plasmid contains a SARS-CoV-2 viral sequence fragment that covers basepairs 8555 to the end of the genome. Because of sequence complexities in nsp3, it is difficult to synthesize the complete nsp3 gene. This construct may be useful to produce RNA controls for assays, as well as serve as templates to make antigens.
Proper citation: RRID:Addgene_153895 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: 43740576
Proper citation: RRID:Addgene_153829 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF6 protein
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: 43740572
Proper citation: RRID:Addgene_153828 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF6 protein
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: 43740572
Proper citation: RRID:Addgene_153827 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: 43740576
Proper citation: RRID:Addgene_153835 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp11
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: 43740578
Proper citation: RRID:Addgene_153833 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: 43740568
Proper citation: RRID:Addgene_153832 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF6 protein
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: 43740572
Proper citation: RRID:Addgene_153839 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: 43740576
Proper citation: RRID:Addgene_153838 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: 43740576
Proper citation: RRID:Addgene_153837 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:4180; Vector Backbone:pTwist_CMV_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: 43740574
Proper citation: RRID:Addgene_153831 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: 43740578
Proper citation: RRID:Addgene_153830 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S (surface glycoprotein)
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: 43740568
Proper citation: RRID:Addgene_153845 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 nsp11
Vector Backbone Description: Backbone Size:6080; Vector Backbone:pTwist_CMV_Puro_6 with AarI site removed; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: 43740578
Proper citation: RRID:Addgene_153840 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: orf10
Vector Backbone Description: Vector Backbone:EF1-alpha-promoter; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34597346
Proper citation: RRID:Addgene_153980 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 M (membrane)
Vector Backbone Description: Vector Backbone:EF1-alpha-promoter; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34597346
Proper citation: RRID:Addgene_153990 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: Orf3a
Vector Backbone Description: Vector Backbone:EF1-alpha-promoter; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34597346
Proper citation: RRID:Addgene_153994 Copy
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