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Genetic Insert: coxM1′::lacZ reporter system with RcoM binding sites "a-f" AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3010 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3010 (coxM1′::lacZ reporter system with binding sites “a-f”) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3010 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37172 Copy
Species: Mustela putorius furo
Genetic Insert: GAD2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3950; Vector Backbone:pCRII-TOPO; Vector Types:cDNA for in situ hybridization; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:20575059
Comments: For riboprobe cut with EcoRI. Runoffs with SP6.
Proper citation: RRID:Addgene_31231 Copy
Species: S. mediterranea
Genetic Insert: Protein arginine methyltransferase 1 homolog
Vector Backbone Description: Backbone Marker:Jim Collins, Newmark lab; Vector Backbone:pJC53.2; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:22318224
Comments: There are several mismatches between Addgene's quality control sequence and the available NCBI sequence. Depositing lab believes these mismatches will not affect plasmid function.
Proper citation: RRID:Addgene_35693 Copy
Species: Capsaspora owczarzaki
Genetic Insert: Capsaspora Histone H2B (CoH2B) fused to Venus
Vector Backbone Description: Backbone Size:5127; Vector Backbone:pONSY; Vector Types:Capsaspora owczarzaki; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29752387
Proper citation: RRID:Addgene_111877 Copy
Genetic Insert: Venus
Vector Backbone Description: Backbone Size:5127; Vector Backbone:pONSY; Vector Types:Capsaspora owczarzaki; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29752387
Proper citation: RRID:Addgene_111875 Copy
Vector Backbone Description: Vector Backbone:pUC18R6K-mini-Tn7T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29742421
Comments: Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_128735 Copy
Species: Homo sapiens
Genetic Insert: KRAS(G12V)
Vector Backbone Description: Vector Backbone:pCR2.1-TOPO cloning vector; Vector Types:homologous recombination donor plasmid; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:25706875
Proper citation: RRID:Addgene_129091 Copy
Species: Cricetulus griseus
Genetic Insert: Beta-(1,4)-Galactosyltransferase 1
Vector Backbone Description: Backbone Marker:Lucigen; Vector Backbone:pJAZZ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31110306
Proper citation: RRID:Addgene_124633 Copy
Species: Cricetulus griseus
Genetic Insert: Alpha-(1,6)-fucosyltransferase
Vector Backbone Description: Backbone Marker:Lucigen; Vector Backbone:pJAZZ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31110306
Proper citation: RRID:Addgene_124631 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 (K218T)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJC45S; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:9674429
Comments: Addgene comment: Please note that the plasmid backbone does not match the pJC45 backbone. The depositor described this plasmid as having a "pJC45S" backbone. Full plasmid sequencing shows features similar to other yeast expression vectors. The lab is closed so were were unable to ask if this difference was intended.
Proper citation: RRID:Addgene_1250 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hsp104 (K620T)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pJC45S; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:9674429
Proper citation: RRID:Addgene_1251 Copy
Species: Streptomyces pristinaespiralis
Genetic Insert: P-furA102tetO-pip
Vector Backbone Description: Backbone Marker:Graham Hatfull (Addgene plasmid # 91723); Backbone Size:5852; Vector Backbone:pTTP1.B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:30962489
Comments: The sequence is that of pTTP1B with P-furA 102 tetO- pip cloned into the EcoRI site.
Proper citation: RRID:Addgene_131105 Copy
Species: bacteriophage P1, EMCV
Genetic Insert: Cre-IRES
Vector Backbone Description: Backbone Marker:Derived in Kotton lab; Backbone Size:7830; Vector Backbone:pHAGE2; Vector Types:Lentiviral, Cre/Lox; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:28965766
Proper citation: RRID:Addgene_126700 Copy
Species: Aspergillus terreus, Escherichia coli, Komagataella phaffii
Genetic Insert: slovA,cpr
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9004; Vector Backbone:pPIC3.5K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31077813
Proper citation: RRID:Addgene_126744 Copy
Species: Aspergillus terreus
Genetic Insert: slovA,cpr
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9004; Vector Backbone:pPIC3.5K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31077813
Proper citation: RRID:Addgene_126743 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: adh2,ald6
Vector Backbone Description: Backbone Marker:Liu Yiqi; Backbone Size:6469; Vector Backbone:pPIC 3.5K(DHis4); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31077813
Proper citation: RRID:Addgene_126737 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: adh2,acs1*
Vector Backbone Description: Backbone Marker:Liu Yiqi; Backbone Size:6469; Vector Backbone:pPIC 3.5K(DHis4); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31077813
Proper citation: RRID:Addgene_126738 Copy
Species: Aspergillus terreus, Aspergillus nidulans
Genetic Insert: atX,npgA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:9004; Vector Backbone:pPIC3.5K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:31077813
Proper citation: RRID:Addgene_126727 Copy
Species: Homo sapiens
Genetic Insert: TP63
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: A549
Proper citation: RRID:Addgene_132477 Copy
Species: Homo sapiens
Genetic Insert: ZNF449
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Comments: Cloning source: K562
Proper citation: RRID:Addgene_132515 Copy
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