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Vector Backbone Description: Backbone Size:12314; Vector Backbone:pPW; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27006187
Proper citation: RRID:Addgene_124238 Copy
Genetic Insert: cat, sacB
Vector Backbone Description: Backbone Size:0; Vector Backbone:pTP809; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:10767554
Comments: pKM154 is a derivative of pBR322 that had sequence between the EcoR1 site and NdeI site removed, with a NotI site inserted (pTP809).
Proper citation: RRID:Addgene_13036 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:5778; Vector Backbone:na; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_13071 Copy
Genetic Insert: 6x Myc Tag
Vector Backbone Description: Backbone Size:5818; Vector Backbone:na; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_13070 Copy
Vector Backbone Description: Backbone Marker:Gregory Newby; Backbone Size:10365; Vector Backbone:pGAN049; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is one of the empty dual yTRAP sensor plasmids. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pSK247. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the LEU2 locus, and can be selected for using 100 ug/ml G418. It can be used in combination with pSK260.
Proper citation: RRID:Addgene_131148 Copy
Vector Backbone Description: Backbone Marker:Szilvia Kiriakov; Backbone Size:10377; Vector Backbone:pSK247; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is the empty dual yTRAP sensor plasmid. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pSK234. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the LEU2 locus, and can be selected for using 100 ug/ml G418. It can be used in combination with an expression clone resulting from LR reaction using pGAN147 basic yTRAP sensor.
Proper citation: RRID:Addgene_131149 Copy
Vector Backbone Description: Backbone Marker:Gregory Newby; Backbone Size:11381; Vector Backbone:pGAN147; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is the empty reverse yTRAP sensor plasmid. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pGAN230. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin. To achieve dual color reporting, combine with pSK221.
Proper citation: RRID:Addgene_131122 Copy
Vector Backbone Description: Backbone Marker:Vidali Lab; Backbone Size:11239; Vector Backbone:pGAPi; Vector Types:RNAi; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32764132
Proper citation: RRID:Addgene_127547 Copy
Species: Other
Genetic Insert: D. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2962; Vector Backbone:Unknown; Vector Types:Insect Expression, Gateway fly expression vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31092928
Proper citation: RRID:Addgene_125153 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 4 x upstream activating sequence (UAS)
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:2500; Vector Backbone:Unknown; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31092928
Proper citation: RRID:Addgene_125150 Copy
Species: Other
Genetic Insert: D. pseudoobscura peak #47 enhancer
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3078; Vector Backbone:Unknown; Vector Types:STAP-seq screening vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31092928
Proper citation: RRID:Addgene_125151 Copy
Vector Backbone Description: Backbone Marker:we built; Backbone Size:10101; Vector Backbone:custom made; Vector Types:Mammalian Expression, Yeast Expression, in vitro expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31467278
Comments: Addgene NGS results identified an IS4-like element located between the Chloramphenicol resistance and ccdB genes. This element will not affect plasmid function. Please visit https://www.biorxiv.org/content/10.1101/530790v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_125549 Copy
Species: E.coli
Genetic Insert: ccdB Cassette (BsaI)
Vector Backbone Description: Vector Backbone:pICH47751; Vector Types:Plant Expression, Binary, Module 1; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31085714
Proper citation: RRID:Addgene_126211 Copy
Vector Backbone Description: Backbone Size:11000; Vector Backbone:pcDNA3.3-TOPO; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_128322 Copy
Species: Aequorea victoria
Genetic Insert: Venus
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:7470; Vector Backbone:pDEST15; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27455993
Comments: 5' cloning site: AgeI (unknown if destroyed), 3' cloning site: MluI (unknown if destroyed)
Proper citation: RRID:Addgene_128567 Copy
Species: Aequorea victoria
Genetic Insert: Venus
Vector Backbone Description: Backbone Marker:ThermoFisher Scientific; Backbone Size:7470; Vector Backbone:pDEST15; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:27455993
Comments: 5' cloning site: AgeI (unknown if destroyed), 3' cloning site: MluI (unknown if destroyed)
Proper citation: RRID:Addgene_128566 Copy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:E.coli/S.aureus shuttle vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16051359
Proper citation: RRID:Addgene_133446 Copy
Genetic Insert: LacZ reporter
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9700; Vector Backbone:pLacZi; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16777607
Comments: Please note mutation R15H in ccdB was found during Addgene's quality control process. The plasmid should function as reported in the associated publication.
Please note that the ccdB cassette in the author's map (click on 'View map') is shown in the reverse orientation.
Y1H Destination vector that can be used in conventional Gateway LR cloning reactions. This vector contains a Gateway cassette with AttR4 and AttL1 recombination sites and a LacZ reporter gene. For more information and protocols, see Deplancke et al. 2004 Genome Res 14(10B):2093 and Deplancke et al. 2006 CSH Protocols.
Proper citation: RRID:Addgene_13350 Copy
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:7720; Vector Backbone:pcDNA-DEST53; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123216 Copy
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:7720; Vector Backbone:pcDNA-DEST53; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30661429
Proper citation: RRID:Addgene_123215 Copy
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