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Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:7678; Vector Backbone:pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for electroporation experiments. Backbone was modified by insertion of N terminal Venus tag at Stu I restriction site
Proper citation: RRID:Addgene_186408 Copy
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:6950; Vector Backbone:pDEST-pSP172BSSPE-Swa1::RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for electroporation experiments. Backbone was modified at Xho I/Xba I restriction site
Proper citation: RRID:Addgene_186407 Copy
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:5605; Vector Backbone:pDEST-Spe3-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of C terminal Venus tag at EcoR V restriction site
Proper citation: RRID:Addgene_186372 Copy
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:5610; Vector Backbone:pDEST-Spe3-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of N terminal Venus tag at Stu I restriction site
Proper citation: RRID:Addgene_186371 Copy
Vector Backbone Description: Backbone Size:5598; Vector Backbone:pDEST-Spe4-RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Gateway destination vector for mRNA production to overexpress a protein of interest following microinjection into ascidian eggs. Likely to be useful for zebrafish, Xenopus, urchin and cnidarian (Clytia, Nematostella...) overexpression studies as well. Backbone was modified by insertion of N terminal mClover3 tag at Bgl II/Stu I restriction site
Proper citation: RRID:Addgene_186379 Copy
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:7673; Vector Backbone:pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for electroporation experiments. Backbone was modified by insertion of C terminal ECFP tag at EcoR V restriction site
Proper citation: RRID:Addgene_186412 Copy
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:6994; Vector Backbone:pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for electroporation experiments. Backbone was modified by insertion of N terminal HA tag at Stu I restriction site
Proper citation: RRID:Addgene_186411 Copy
Species: Synthetic
Genetic Insert: sgRNA-scaffold only
Vector Backbone Description: Backbone Marker:Takara; Backbone Size:2125; Vector Backbone:pMD19; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35969224
Proper citation: RRID:Addgene_190794 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4650; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28060929
Proper citation: RRID:Addgene_190226 Copy
Vector Backbone Description: Vector Backbone:pMOD_B0000; Vector Types:cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176874 Copy
Vector Backbone Description: Vector Backbone:pMOD_C'0000a; Vector Types:cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36253612
Comments: MoClo compatible
Proper citation: RRID:Addgene_176875 Copy
Vector Backbone Description: Vector Backbone:pMOD_B0000; Vector Types:cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36253612
Comments: MoClo compatible, allows transition from MoClo cloning system into the Cermak, et al., 2017 Golden Gate assembly system
Proper citation: RRID:Addgene_176883 Copy
Vector Backbone Description: Backbone Marker:PMID: 17878951; Backbone Size:4677; Vector Backbone:pDEST-pSP172BSSPE-Swa1::RfA; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17878951
Comments: Gateway destination vector for electroporation experiments. Backbone was modified by insertion of N terminal HA tag at Stu I restriction site
Proper citation: RRID:Addgene_186404 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4650; Vector Backbone:pAAV-MCS; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:28060929
Proper citation: RRID:Addgene_190238 Copy
Vector Backbone Description: Backbone Marker:Stephen Elledge (Addgene #44012); Backbone Size:11874; Vector Backbone:pINDUCER20; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: C terminal 3x Flag on backbone
Proper citation: RRID:Addgene_190028 Copy
Vector Backbone Description: Backbone Marker:David Root (Addgene plasmid # 41393); Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_194065 Copy
Vector Backbone Description: Backbone Marker:David Root (Addgene plasmid # 41393); Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_194071 Copy
Vector Backbone Description: Backbone Marker:David Root (Addgene plasmid # 41393); Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_194074 Copy
Vector Backbone Description: Vector Backbone:pcg2.0; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31308540
Proper citation: RRID:Addgene_180221 Copy
Vector Backbone Description: Backbone Marker:Thermofisher; Backbone Size:7603; Vector Backbone:pCDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:35182477
Proper citation: RRID:Addgene_182926 Copy
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