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Species: Synthetic
Genetic Insert: SSX1p-mKate2
Vector Backbone Description: Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29056342
Proper citation: RRID:Addgene_105172 Copy
Genetic Insert: UbCp-rtTA3
Vector Backbone Description: Backbone Size:9000; Vector Backbone:FUGW; Vector Types:Mammalian Expression, Lentiviral, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29056342
Proper citation: RRID:Addgene_105171 Copy
Vector Backbone Description: Backbone Marker:Clontech = pTRE-T2; Backbone Size:5041; Vector Backbone:pTRE-T2-mIR-Hygromycin; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: A synthetic DNA containing the IRES element was cloned into EcoRI/Xba I site of pTRE-T2-miR-HYGRO vector after removal of the mIR cassette. First digest parental pTRE-T2-miR-HYGRO vector with EcoRI and Xba I to remove the miR cassette. Then the IRES cassette was ligated back to the EcoRI site and Xba I site. The IRES cassette contains the unique EcoRI site followed by unique NotI and Mlu I sites that is followed by the start of the IRES sequence. This cloning site will allow insertion of the 1st cDNA. On the 3’ end of IRES there are unique Spe I and Xba I cloning sites for insertion of the second cDNA into the cassette.
Please note- to use the BsaI and XbaI sites for cloning, you must use unmethylated DNA. You will need to isolate the DNA from a methylation deficient bacterial strain.
Proper citation: RRID:Addgene_105169 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: kanMX6-3nmt1-mMaple3
Vector Backbone Description: Backbone Size:4170; Vector Backbone:pFA6a; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27385337
Comments: GlySerGly are inserted before mMaple3 The sizes of backbone and inserts are approximate, and do not include the selectable marker.
Proper citation: RRID:Addgene_105167 Copy
Species: Mus musculus
Genetic Insert: Rictor
Vector Backbone Description: Backbone Marker:Yusa Lab; Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuroBFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29996108
Proper citation: RRID:Addgene_105042 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: hphMX6-41nmt1
Vector Backbone Description: Backbone Size:4170; Vector Backbone:pFA6a; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22298427
Comments: The sizes of backbone and inserts are approximate, and do not include the selectable marker.
Proper citation: RRID:Addgene_105163 Copy
Species: Mus musculus
Genetic Insert: Stk11
Vector Backbone Description: Backbone Marker:Yusa Lab; Vector Backbone:pKLV2-U6gRNA5(BbsI)-PGKpuroBFP-W; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29996108
Proper citation: RRID:Addgene_105043 Copy
Species: Schizosaccharomyces pombe
Genetic Insert: hphMX6-81nmt1
Vector Backbone Description: Backbone Size:4170; Vector Backbone:pFA6a; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22298427
Comments: The sizes of backbone and inserts are approximate, and do not include the selectable marker.
Proper citation: RRID:Addgene_105164 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Pik1
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105260 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Chs3 portion
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105257 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gga2 portion
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105258 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Hse1 portion
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105259 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Vps8 portion
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105272 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Vph1 portion
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105270 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Sec7 portion
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Comments: Addgene's quality control identified a frameshift at Q266 of URA3. The depositing lab states that this mutation should not affect selection.
Proper citation: RRID:Addgene_105268 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mup1 portion
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105266 Copy
Genetic Insert: Kex2 portion
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105264 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Vps21
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Comments: Addgene's quality control identified R262G in URA3. The depositing lab states that this mutation should not affect selection.
Proper citation: RRID:Addgene_105265 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Tlg2
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105262 Copy
Genetic Insert: Ypt31
Vector Backbone Description: Backbone Size:3797; Vector Backbone:YIplac211; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29316441
Proper citation: RRID:Addgene_105263 Copy
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