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Species: E. coli
Genetic Insert: LetAB
Vector Backbone Description: Vector Backbone:pET17b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32359438
Comments: Please visit https://www.biorxiv.org/content/10.1101/748145v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_139896 Copy
Species: E. coli
Genetic Insert: LexA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15203006
Proper citation: RRID:Addgene_14593 Copy
Species: E. coli
Genetic Insert: LexA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5600; Vector Backbone:pcDNA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15203006
Proper citation: RRID:Addgene_14591 Copy
Species: E. coli
Genetic Insert: MBP
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16581772
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146120 Copy
Species: E. Coli
Genetic Insert: E Coli tRNA Guanine Transglycosylase-His
Vector Backbone Description: Backbone Marker:IDT; Backbone Size:3000; Vector Backbone:pUCIDT-Kan; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32713531
Comments: J. Am. Chem. Soc. 2015, 137, 40, 12756-12759
Proper citation: RRID:Addgene_138201 Copy
Species: E. coli
Genetic Insert: mlaFEDCB
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:4571; Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28388411
Proper citation: RRID:Addgene_138526 Copy
Species: E. coli
Genetic Insert: mfd
Vector Backbone Description: Backbone Size:5344; Vector Backbone:pskB2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16511235
Proper citation: RRID:Addgene_134349 Copy
Species: E. coli
Genetic Insert: Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
Vector Backbone Description: Backbone Marker:Hotta Lab at CiRA, Kyoto University; Vector Backbone:pPV piggyBac vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31811138
Proper citation: RRID:Addgene_134923 Copy
Species: E. coli
Genetic Insert: Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
Vector Backbone Description: Backbone Marker:Hotta Lab at CiRA, Kyoto University; Vector Backbone:pPV piggyBac vector; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31811138
Proper citation: RRID:Addgene_134924 Copy
Species: E. coli
Genetic Insert: SSB-GFP
Vector Backbone Description: Backbone Size:5407; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32374866
Proper citation: RRID:Addgene_136471 Copy
Species: E. coli
Genetic Insert: SSB-C-term-GFP
Vector Backbone Description: Backbone Size:5382; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32374866
Proper citation: RRID:Addgene_136472 Copy
Species: E. coli
Genetic Insert: Prha:mVenus
Vector Backbone Description: Backbone Marker:Anna Behle; Vector Backbone:pSHDY; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32134640
Comments: Please visit https://www.biorxiv.org/content/10.1101/757948v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_137662 Copy
Species: E. coli
Genetic Insert: Lvl0 TetR-Gly9-DDX6 (contains SapI)
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30327560
Proper citation: RRID:Addgene_115949 Copy
Species: E. coli
Genetic Insert: E. coli gRNAs
Vector Backbone Description: Vector Backbone:psgRNAcos; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29765036
Proper citation: RRID:Addgene_115927 Copy
Species: E. coli
Genetic Insert: Colicin Ia + Ia immunity protein
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2686; Vector Backbone:PUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19919671
Comments: The original clone had some mutations that had been introduced during the PCR cloning of the colicin operon (into another vector). Here, the operon was moved into pUC19 and the mutations were corrected, so that the sequence is the same as the one in GenBank (M13819.1)
This plasmid contains Colicin Ia + Ia immunity protein, each with their own promoters; colicin production can be induced with mitomycin C.
The SOS promoter for colicin expression is at around residues 148-165 of the insert, in the forward direction. The immunity promoter -35 box is about 460 bp from the end of the insert; there are only about 20 bp between the terminator for the colicin gene, reading in one direction, and the terminator for the immunity protein, reading in the other.
Proper citation: RRID:Addgene_27125 Copy
Species: E. coli
Genetic Insert: grpE
Vector Backbone Description: Backbone Size:4493; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
Proper citation: RRID:Addgene_27391 Copy
Species: E. coli
Genetic Insert: dnaK, dnaJ, groESL
Vector Backbone Description: Backbone Size:9177; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17565681
Comments: Between depositor sequence and Addgene sequencing results, there is a mismatch at position 7284 and an insertion at position 7360. These are in vector backbone and do not alter expression of the inserts.
Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
Proper citation: RRID:Addgene_27395 Copy
Species: E. coli
Genetic Insert: dnaK, dnaJ, GroESL
Vector Backbone Description: Backbone Size:9203; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
single nt insertion at position 1726.
Proper citation: RRID:Addgene_27396 Copy
Species: E. coli
Genetic Insert: grpE, clpB
Vector Backbone Description: Backbone Size:7194; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
Proper citation: RRID:Addgene_27393 Copy
Species: E. coli
Genetic Insert: reverse tetracycline-regulated transactivator advanced
Vector Backbone Description: Backbone Marker:unpublished; Backbone Size:7200; Vector Backbone:pMA2635rc; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19655272
Proper citation: RRID:Addgene_25435 Copy
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