Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS415; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14409 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS414; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14407 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS425; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14417 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS424; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14415 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS423; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14413 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS415; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: CEN.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14410 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pRS426; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17583893
Comments: Type of plasmid: 2-micron.
This plasmid contains the Gateway technology from Invitrogen; Invitrogen requires purchasing of Gateway Clonase for carrying out the Gateway recombinational cloning reaction http://www.invitrogen.com
Please note that the full plasmid sequence is provided as a reference only, and may not match the exact sequence of the plasmid.
Proper citation: RRID:Addgene_14419 Copy
Vector Backbone Description: Backbone Marker:Addgene Plasmid #49386; Backbone Size:6198; Vector Backbone:pcDNA3 APEX2-NES; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29625033
Proper citation: RRID:Addgene_149517 Copy
Species: Synthetic
Genetic Insert: PGK-puroR-pA cassette, inserted in PB-TET vector
Vector Backbone Description: Backbone Marker:Andras Nagy (Addgene plasmid # 20909); Backbone Size:13983; Vector Backbone:PB-TET (addgene #20909); Vector Types:Mammalian Expression, piggyBac; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_139561 Copy
Vector Backbone Description: Backbone Marker:Linzhao Cheng (Addgene plasmid # 28213); Backbone Size:9114; Vector Backbone:pEB-C5 (Addgene #28213); Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: ccdB-Cm cassette was inserted on the backbone
Proper citation: RRID:Addgene_139519 Copy
Vector Backbone Description: Backbone Size:4895; Vector Backbone:pCAGGS; Vector Types:Cre/Lox; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: ccdB-Cm cassette was inserted between loxP
Proper citation: RRID:Addgene_139517 Copy
Vector Backbone Description: Backbone Marker:Linzhao Cheng (Addgene plasmid # 28213); Backbone Size:11319; Vector Backbone:pEB-C5 (Addgene #28213); Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: ccdB-Cm cassette was inserted on the backbone
Proper citation: RRID:Addgene_139520 Copy
Vector Backbone Description: Backbone Size:4338; Vector Backbone:pMC1DT-ApA; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: ccdB-Cm cassette was inserted on the backbone; Uses Diphtheria toxin A (DTA) as negative selection.
Proper citation: RRID:Addgene_139521 Copy
Vector Backbone Description: Backbone Size:0; Vector Backbone:pWZL-Neo-Myr-Flag-DEST; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17574021
Comments: pWZL-Neo-Myr-Flag-DEST (pWN-MF-DEST) is a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF.
Created by inserting an N-terminal myr-FLAG tag and the Gateway-selectable cassette into the pWzl-Neo retroviral vector. To create specific pWN-MF-ORF vectors, perform site-specific LR recombination reactions.
LR reaction:
150ng DEST vector + 50ng ENTRY vector + 10xLR Buffer + LR enzyme + dH20. Incubate at 25oC for 1 hour. Add 1uL Proteinase K (20mg/ml), and incubate at 37oC for 10min. Transform into DH5alpha cells. See Invitrogen's website for detailed protocols and for LR enzyme.
Proper citation: RRID:Addgene_15300 Copy
Vector Backbone Description: Backbone Size:9866; Vector Backbone:pAWH; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30217970
Proper citation: RRID:Addgene_133894 Copy
Vector Backbone Description: Backbone Size:11698; Vector Backbone:pCR8/GW/TOPO; Vector Types:Mammalian Expression, Other, Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134975 Copy
Vector Backbone Description: Backbone Size:11095; Vector Backbone:pCR8/GW/TOPO; Vector Types:Mammalian Expression, Other, Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134976 Copy
Vector Backbone Description: Backbone Size:4723; Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134978 Copy
Genetic Insert: Gateway cassette
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:4720; Vector Backbone:pQE-2; Vector Types:Bacterial Expression, Co-Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17311810
Comments: Please note that the chloramphenicol resistance gene outside of the attB sites in this plasmid is incomplete. It is lacking a promoter, therefore it is supposed to be inactive.
Proper citation: RRID:Addgene_13668 Copy
Genetic Insert: Gateway cassette
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:5356; Vector Backbone:pQE-2; Vector Types:Bacterial Expression, Co-Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17311810
Comments: Please note that the chloramphenicol resistance gene outside the attB sites in this plasmid is incomplete. It is lacking a promoter, therefore it is supposed to be inactive.
Proper citation: RRID:Addgene_13673 Copy
Can't find your Plasmid?
We recommend that you click next to the search bar to check some helpful tips on searches and refine your search firstly. If you want to find a specific plasmid, it's easier to enter an RRID or an Addgene Catalog Number to search. You can refine the search results using Facets on the left side of the search results page. If you are on the table view, you can also search in a specific column by clicking the column title and enter the keywords.
If you still could not find your plasmid in the search results, please help us by registering it into the system — it's easy. Register it with Addgene.
Welcome to the dkNET Resources search. From here you can search through a compilation of resources used by dkNET and see how data is organized within our community.
You are currently on the Community Resources tab looking through categories and sources that dkNET has compiled. You can navigate through those categories from here or change to a different tab to execute your search through. Each tab gives a different perspective on data.
If you have an account on dkNET then you can log in from here to get additional features in dkNET such as Collections, Saved Searches, and managing Resources.
Here is the search term that is being executed, you can type in anything you want to search for. Some tips to help searching:
You can save any searches you perform for quick access to later from here.
We recognized your search term and included synonyms and inferred terms along side your term to help get the data you are looking for.
If you are logged into dkNET you can add data records to your collections to create custom spreadsheets across multiple sources of data.
Here are the sources that were queried against in your search that you can investigate further.
Here are the categories present within dkNET that you can filter your data on
Here are the subcategories present within this category that you can filter your data on
If you have any further questions please check out our FAQs Page to ask questions and see our tutorials. Click this button to view this tutorial again.