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Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp4
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725300.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145495 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp9
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725305.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145496 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp9
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725305.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145493 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 endoRNAse
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725310.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145538 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725318.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145547 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF7b
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725318.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145548 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 endoRNAse
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725310.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145545 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 leader protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725297.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145543 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF3a protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009724391.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145544 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 3C-like proteinase
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725301.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145541 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 leader protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725297.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145542 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 nsp7
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725303.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145540 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SARS-CoV-2 ORF10 protein
Vector Backbone Description: Backbone Size:4078; Vector Backbone:AmpR; 2u URA3; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: NCBI Reference Sequence: YP_009725255.1Created by gene synthesis with Not1 site.
Proper citation: RRID:Addgene_145549 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnt1
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33751023
Proper citation: RRID:Addgene_171552 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnt1
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33751023
Proper citation: RRID:Addgene_171550 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Rnt1
Vector Backbone Description: Vector Backbone:pGEX-4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33751023
Proper citation: RRID:Addgene_171549 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: SSA4-U1A-ASH1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: SSA4 promoter and SSA4 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), ASH1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1371 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PGK1-U1A-ASH1 3' UTR
Vector Backbone Description: Backbone Size:5726; Vector Backbone:pRS426; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11142374
Comments: PGK1 promoter and PGK1 ORF (XhoI/BamHI fragment), U1A hairpins (BamHI/BglII fragment), ASH1 3' UTR (SpeI/NotI fragment), ADH2 terminator (NotI/SacII fragment), 2 micron plasmid.
Proper citation: RRID:Addgene_1372 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Signal recognition particle receptor β subunit
Vector Backbone Description: Backbone Size:5332; Vector Backbone:pET28a-derived; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16627619
Proper citation: RRID:Addgene_13729 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Signal recognition particle receptor β subunit
Vector Backbone Description: Backbone Size:5312; Vector Backbone:pET28a derived; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16627619
Comments: Construct is expressed with a precission protease cleavable 6xHis tag
Proper citation: RRID:Addgene_13727 Copy
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