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Species: Synthetic
Genetic Insert: human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
Vector Backbone Description: Backbone Marker:Cepko Lab (Addgene plasmid #11179); Vector Backbone:pCAG-CFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_114079 Copy
Species:
Genetic Insert: Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
Vector Backbone Description: Backbone Size:6235; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_114111 Copy
Species: Homo sapiens
Genetic Insert: SHLD2
Vector Backbone Description: Backbone Size:5117; Vector Backbone:pcDNA5-FRT/TO-FLAG; Vector Types:Mammalian Expression, Genomic integration, tetracycline inducible; Bacterial Resistance:Ampicillin
References:
Comments: The SHLD2 short isoform cDNA was obtained from the ORFeome collection. The complete coding sequence of the long isoform of SHLD2 was generated by combining a synthesized fragment corresponding to the long isoform C-terminus using an internal KpnI site.
Proper citation: RRID:Addgene_114117 Copy
Species: Homo sapiens
Genetic Insert: SHLD1
Vector Backbone Description: Backbone Size:5152; Vector Backbone:pcDNA5-FRT/TO-FLAG; Vector Types:Mammalian Expression, Genomic integration, tetracycline inducible; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_114115 Copy
Species: Synthetic
Genetic Insert: DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.3)
Vector Backbone Description: Backbone Marker:Cepko Lab (Addgene plasmid #11179); Vector Backbone:pCAG-CFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_114083 Copy
Species:
Genetic Insert: Gaussia luciferase, slow burn; Channelrhodopsin 2(CS/DA), stable step function opsin; EYFP
Vector Backbone Description: Backbone Size:4610; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_114106 Copy
Species:
Genetic Insert: sbGLuc-VChR1-EYFP
Vector Backbone Description: Backbone Size:4615; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_114104 Copy
Species:
Genetic Insert: sbGLuc-VChR1-EYFP
Vector Backbone Description: Backbone Size:4552; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_114103 Copy
Species:
Genetic Insert: Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
Vector Backbone Description: Backbone Size:4269; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_114108 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments: Accession Number MG649424 , Alternative ID = pGT403 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114164 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments: Accession Number MG649422, Alternative ID = pGT401 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114162 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:2658; Vector Backbone:pUC19; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
References:
Comments: Accession Number MG649420, Alternative ID = pGT400 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114161 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments: Accession Number MG649425 , Alternative ID = pGT406 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114167 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:3488; Vector Backbone:pACYC184; Vector Types:Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments: Accession Number MG649426, Alternative ID = pGT405 . Please visit https://www.biorxiv.org/content/early/2018/07/04/361626 for bioRxiv preprint.
Proper citation: RRID:Addgene_114166 Copy
Species: M. jannaschii
Genetic Insert: RNase HII
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the plasmid pET21a.MJ0135 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11412 Copy
Species: E. coli
Genetic Insert: clbO
Vector Backbone Description: Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_114153 Copy
Species: E. coli
Genetic Insert: clbK
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_114152 Copy
Species: M. jannaschii
Genetic Insert: 3-hexulose-6-phosphate isomerase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the plasmid pET21a.MJ1247 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11414 Copy
Species: M. jannaschii
Genetic Insert: phosphoserine phosphatase
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5443; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Please note that the plasmid pET21a.MJ1594 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
Proper citation: RRID:Addgene_11413 Copy
Species: E. coli
Genetic Insert: clbH
Vector Backbone Description: Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_114157 Copy
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