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Species: Synthetic
Genetic Insert: ASAP2s
Vector Backbone Description: Backbone Size:4562; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
References:
Comments: The next-generation JEDI-2P sensors are available at: https://www.addgene.org/browse/article/28223314/
Proper citation: RRID:Addgene_101276 Copy
Species: Homo sapiens
Genetic Insert: METTL13
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #62304); Vector Backbone:pFBOH-MHL; Vector Types:Baculovirus Expression; Bacterial Resistance:Ampicillin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: METTL13:PBC007-B09:C235684. SGC or PDB link: http://www.thesgc.org/structures/5WCJ/
Proper citation: RRID:Addgene_101270 Copy
Species: Homo sapiens
Genetic Insert: EPHA3
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26094); Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: N terminal tag: MGSSHHHHHHSSGLVPRGS. SGC Clone Sample ID: EPHA3:SDC033-F08:C12121. SGC or PDB link: http://www.rcsb.org/pdb/explore/explore.do?structureId=2QO2
Proper citation: RRID:Addgene_101271 Copy
Species: Other
Genetic Insert: 4PY6
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26092); Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: PFA0510w:MAC02W-B09:C201930. SGC or PDB link: http://www.rcsb.org/pdb/explore/explore.do?structureId=4PY6
Proper citation: RRID:Addgene_101272 Copy
Species: Mesoplasma florum
Genetic Insert: oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_101316 Copy
Species: Mesoplasma florum
Genetic Insert: oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_101310 Copy
Species: Mus musculus
Genetic Insert: E-cadherin
Vector Backbone Description: Backbone Marker:J. Bear (UNC Chapel Hill, USA); Vector Backbone:LentiLox pLL5.0(backbone pLL3.7); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101278 Copy
Species: Mesoplasma florum
Genetic Insert: oriC3 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, w/o dnaA)
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
References:
Comments:
Proper citation: RRID:Addgene_101311 Copy
Species: Homo sapiens
Genetic Insert: Utrophin
Vector Backbone Description: Vector Backbone:pCS2.0; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101279 Copy
Species: Other
Genetic Insert: NL4-3 vpr
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101329 Copy
Species: Synthetic
Genetic Insert: MRLC-DD and AP4-Mito
Vector Backbone Description: Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: MRLC-DD: Myosin regulatory light-chain transgene and AP4-Mito:a transgene bearing ActA AP4 motifs fused to a mitochondrial targeting sequence (INACTIVE)
Proper citation: RRID:Addgene_101287 Copy
Species: Mus musculus
Genetic Insert: E-Cadherin-GFP-N-WASP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: was generated by recombinining mEcad-GFP, a linker5"-GGGTCTACATCTGGATCTGGGAGGCCGGGTTCTGGTGAGGGTTCT3 and N-WASPdeltaVCA into NOT1/Age Iin gfp-N1 vector
Proper citation: RRID:Addgene_101283 Copy
Species: Other
Genetic Insert: JR-CSF env
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101325 Copy
Species: Other
Genetic Insert: NL4-3 rev
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101327 Copy
Species: Mus musculus
Genetic Insert: Rock1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: The GFP-ROCK1 construct was generated by PCR-amplifying mouse ROCK1 using FLAG-tag mouse ROCK1 (a kind gift from M. Samuel, Centre for Cancer Biology, University of South Australia, Australia) as a template and cloned into pEGFP-C1 (Clontech) using SacI and KpnI restriction sites.
Proper citation: RRID:Addgene_101289 Copy
Species: Other
Genetic Insert: eGFP
Vector Backbone Description: Vector Backbone:pALPS; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101323 Copy
Species: KSHV (viral)
Genetic Insert: LANApi TATA box mutation in pBluescript-LANApi,K14 (pDD2000).
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
References:
Comments: Addgene's sequencing found Y108H and G360D mutations in CBG68luc. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_101372 Copy
Species: KSHV (viral)
Genetic Insert: RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
References:
Comments: Addgene's sequencing found V466A and R510C mutations in CBG68luc and a V298A mutation in CBRluc. These mutations are not known to affect plasmid function.
Proper citation: RRID:Addgene_101373 Copy
Species: Homo sapiens
Genetic Insert: RPUSD4
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: RPUSD4:JMC111-H03:C235563. SGC or PDB link: http://www.thesgc.org/structures/5UBA/
Proper citation: RRID:Addgene_101256 Copy
Species: KSHV (viral)
Genetic Insert: Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), 611-843 REV
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:7150; Vector Backbone:pBluescript II (KS+); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_101377 Copy
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