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Species: Drosophila melanogaster
Genetic Insert: spaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: There are several polymorphisms in the 3’UTR of the expression construct and should not affect the function.
Proper citation: RRID:Addgene_20163 Copy
Species: Mus musculus
Genetic Insert: C-Fluc
Vector Backbone Description: Backbone Marker:Ohlfest Lab; Backbone Size:6082; Vector Backbone:colE1/ampR; Vector Types:Transposon; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20203 Copy
Species: E. coli
Genetic Insert: RuvC
Vector Backbone Description: Backbone Marker:made by S Tabor/sold by USB; Backbone Size:2743; Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20169 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9637; Vector Backbone:pGADT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
References:
Comments: Two mutations are present in the LEU2 sequence: D52E and V69A
Proper citation: RRID:Addgene_20161 Copy
Species: Homo sapiens
Genetic Insert: CFP-FKBP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:ECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20160 Copy
Species: E. coli
Genetic Insert: DHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: murine VH chain signal peptide followed by a HA
tag and one repeat of DHFR
Proper citation: RRID:Addgene_20217 Copy
Species: E. coli
Genetic Insert: 3xDHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20216 Copy
Species: Homo sapiens
Genetic Insert: src homology domain 3
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:4500; Vector Backbone:pRSF-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20177 Copy
Species: Homo sapiens
Genetic Insert: TNFa 3' UTR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments: The 3′UTR was generated by PCR amplification from a liver cDNA library (Invitrogen). and the expression plasmids pMC-TNFα was constructed by inserting the 3′UTR of TNFα downstream of CAT in the pMC construct.
Proper citation: RRID:Addgene_20210 Copy
Species: Homo sapiens
Genetic Insert: src homology domain 3
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:4500; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
References:
Comments: Gene 1998 224 45-52
Proper citation: RRID:Addgene_20176 Copy
Species: Homo sapiens
Genetic Insert: pEX-CMV-SP-YFP-STM2(15-746AA)-EF hand 3 mut (K83A,D84N,G86D)
Vector Backbone Description: Backbone Size:0; Vector Backbone:Gateway destination vector; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments: STIM2 also has P38L, V222I, and E575V, as described in the paper's supplemental material. These mutations do not affect function.
Proper citation: RRID:Addgene_20174 Copy
Species: E. coli
Genetic Insert: DHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20214 Copy
Species: Homo sapiens
Genetic Insert: 3xFKBP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pcDNA3.1/Zeo(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20213 Copy
Species: Homo sapiens
Genetic Insert: C-CrkII nSH3
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:4500; Vector Backbone:pRSF-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
References:
Comments: Gene 1998 224 45-52
Proper citation: RRID:Addgene_20178 Copy
Species: Mus musculus
Genetic Insert: pDEST27-Vinculin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:8123; Vector Backbone:pDEST27; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20144 Copy
Species: Homo sapiens
Genetic Insert: cell division cycle 42 isoform 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20142 Copy
Species: Homo sapiens
Genetic Insert: YFP-FRB (YR)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:EYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
References:
Comments:
Proper citation: RRID:Addgene_20148 Copy
Species: Homo sapiens
Genetic Insert: Lyn-linker-FRB (LDR)
Vector Backbone Description: Backbone Marker:ARIAD Pharmaceuticals; Backbone Size:0; Vector Backbone:pC4-RHE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20147 Copy
Species:
Genetic Insert: Artificial sequence
Vector Backbone Description: Backbone Marker:Stratagene (now Agilent); Backbone Size:3469; Vector Backbone:pSC-B; Vector Types:Cre/Lox; Bacterial Resistance:Ampicillin
References:
Comments: May linearize backbone using the single XmnI site.
Proper citation: RRID:Addgene_20145 Copy
Species: Mus musculus
Genetic Insert: pcDNA3.1/nV5-DEST-beta catenin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7136; Vector Backbone:pcDNA3.1/nV5-DEST; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
References:
Comments:
Proper citation: RRID:Addgene_20140 Copy
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