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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pDFC(P0) Resource Report Resource Website |
RRID:Addgene_251363 | Spectinomycin and Streptomycin | PMID:41699564 | Vector Backbone:pDFC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | Addgene | 2026-09-26 02:54:22 | 0 | ||||
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pIN205 Resource Report Resource Website |
RRID:Addgene_255796 | NrdJ-1C - XtpS | Xenorhabdus nematophila ATCC 19061 | Spectinomycin and Streptomycin | This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUTII site and fused to the C-terminal part of the split intein NrdJ-1.When co-expressed with pIN092 and pIN201, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol). For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains. Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose. | Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | Addgene | 2026-09-26 02:55:19 | 0 | ||
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pIN207 Resource Report Resource Website |
RRID:Addgene_255798 | SspGyrBC - XtpS | Xenorhabdus nematophila ATCC 19061 | Spectinomycin and Streptomycin | This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XU site and fused to the C-terminal part of the split intein SspGyrB.When co-expressed with pIN103 and pIN203, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol). For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains. Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose. | Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | Addgene | 2026-09-26 02:55:19 | 0 | ||
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pART27::CLOMELEON Resource Report Resource Website |
RRID:Addgene_247988 | CLOMELEON (CFP-YFP fusion with ENLYFQG-linker) | Synthetic | Spectinomycin and Streptomycin | PMID:15086798 | pART27::CLOMELEON is a binary pART27 vector harbouring a NotI-fragment with a gene cassette subcloned from pART7::CLOMELEON to stably insert the CLOMELEON gene (Kuner, T. & Augustine, G.J.; PubMed: 11055428; doi: 10.1016/S0896-6273(00)00056-8) into the genome of plants by Agrobacterium-mediated gene transfer, e.g. by floral dip (Clough & Bent 1998; PubMed: 10069079; doi: 10.1007/BF00028910). See the Supplemental Document under Resource Information for more details, including annotated maps. | Backbone Marker:Andrew Gleave; Backbone Size:11612; Vector Backbone:pART27; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin | CFP-variant: K26R, F64L, S65T, Y66W, N146I, M153T, V163A, N164H, H231L; YFP-variant: S65G, S72A, K79R, T203Y, H231L | Addgene | 2026-09-26 02:53:43 | 0 |
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pEA106 Resource Report Resource Website |
RRID:Addgene_187872 | mCherry | Spectinomycin and Streptomycin | PMID:35690588 | Backbone Marker:Keunsub Lee; Backbone Size:6149; Vector Backbone:pTF505; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | Addgene | 2026-09-26 02:42:07 | 0 |
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