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Genetic Insert: SARS-CoV-2-S2P-B.1.429
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176329 Copy
Genetic Insert: SARS-CoV-2-S2P-B.1.1.7
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176325 Copy
Genetic Insert: SARS-CoV-2-RBD-SD1-L452R
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176452 Copy
Genetic Insert: SARS-CoV-2-RBD-SD1-S477N
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176453 Copy
Genetic Insert: SARS-CoV-2-RBD-SD1-L452R-T478K
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176456 Copy
Genetic Insert: SARS-CoV-2-RBD-SD1-L452R-E484Q
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176455 Copy
Genetic Insert: SARS-CoV-2-RBD-SD1-WA1
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176448 Copy
Genetic Insert: SARS-CoV-2-RBD-K417N-E484K-N501Y
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176440 Copy
Genetic Insert: SARS-CoV-2-RBD-S477N
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176443 Copy
Genetic Insert: SARS-CoV-2-RBD-L452R-T478K
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176446 Copy
Species: Homo sapiens
Genetic Insert: VPS13D
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3679; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33891012
Comments: GFP-Ub-VPS13D will be translated into one fusion protein. However, endogenous DUBs will cleave it into GFP-Ub and VPS13D fragments. GFP-Ub hence acts as a fluorescent reporter for VPS13D expression.
Proper citation: RRID:Addgene_176462 Copy
Species: Homo sapiens
Genetic Insert: VPS13D
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3679; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33891012
Comments: GFP-Ub-VPS13D will be translated into one fusion protein. However, endogenous DUBs will cleave it into GFP-Ub and VPS13D fragments. GFP-Ub hence acts as a fluorescent reporter for VPS13D expression.
Proper citation: RRID:Addgene_176465 Copy
Species: Homo sapiens
Genetic Insert: VPS13D
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:3679; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33891012
Comments: EYFP is internally inserted to replace the alternative skipping exon 40 of VPS13D (in-frame)
Proper citation: RRID:Addgene_176464 Copy
Species: Ktedonobacter racemifer
Genetic Insert: non-coding locus components from KraIscB-1 locus
Vector Backbone Description: Backbone Size:3719; Vector Backbone:pCOLADuet1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34591643
Proper citation: RRID:Addgene_176537 Copy
Species: Homo sapiens
Genetic Insert: D50 lamin A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4730; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15750600
Proper citation: RRID:Addgene_17653 Copy
Genetic Insert: SARS-CoV-2-NTD-B.1.618
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176438 Copy
Genetic Insert: SARS-CoV-2-RBD-N501Y
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176439 Copy
Genetic Insert: SARS-CoV-2-NTD-W152C
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176431 Copy
Genetic Insert: SARS-CoV-2-NTD-G142D-E154K
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176434 Copy
Genetic Insert: SARS-CoV-2-NTD-E154K
Vector Backbone Description: Vector Backbone:pVRC8400; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609088
Proper citation: RRID:Addgene_176433 Copy
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