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Genetic Insert: xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
Vector Backbone Description: Vector Backbone:pSEVA448; Vector Types:Pseudomonas vector; Bacterial Resistance:Streptomycin
Defining Citation: PMID:38180826
Proper citation: RRID:Addgene_207524 Copy
Genetic Insert: oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP;
Vector Backbone Description: Backbone Marker:SEVA collection; Vector Backbone:pSEVA448; Vector Types:CRISPR, Pseudomonas vector; Bacterial Resistance:Streptomycin
Defining Citation: PMID:38180826
Proper citation: RRID:Addgene_207525 Copy
Genetic Insert: Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
Vector Backbone Description: Backbone Marker:SEVA collection; Vector Backbone:pSEVA448; Vector Types:CRISPR, Pseudomonas vector; Bacterial Resistance:Streptomycin
Defining Citation: PMID:38180826
Proper citation: RRID:Addgene_207531 Copy
Genetic Insert: RhaR
Vector Backbone Description: Backbone Marker:Dongru Qiu, Hongwei D. Yu; Backbone Size:5216; Vector Backbone:pHERD30T; Vector Types:Mammalian Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:37975669
Comments: Please visit https://doi.org/10.1101/2023.06.29.547033 for bioRxiv preprint.
Proper citation: RRID:Addgene_208001 Copy
Species: Acetobacter aceti 1023
Genetic Insert: formylglycinamidine ribonucleotide synthetase, small subunit
Vector Backbone Description: Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Comments: pCloDF13 replicon
Proper citation: RRID:Addgene_72516 Copy
Species: Acetobacter aceti 1023
Genetic Insert: formylglycinamidine ribonucleotide synthetase, small subunit
Vector Backbone Description: Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Comments: pCloDF13 replicon
Proper citation: RRID:Addgene_72517 Copy
Species: Acetobacter aceti 1023
Genetic Insert: formylglycinamidine ribonucleotide synthetase, large subunit
Vector Backbone Description: Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Comments: pCloDF13 replicon
Proper citation: RRID:Addgene_72520 Copy
Species: Acetobacter aceti 1023
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide mutase
Vector Backbone Description: Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Comments: CDF ori
Proper citation: RRID:Addgene_72450 Copy
Species: Acetobacter aceti 1023
Genetic Insert: N5-carboxyaminoimidazole ribonucleotide synthetase
Vector Backbone Description: Backbone Size:3781; Vector Backbone:pCDFDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Comments: CDF ori
Proper citation: RRID:Addgene_72449 Copy
Species: from K12 genome
Genetic Insert: Cas1, Cas2
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pCDF-1b; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:22402487
Proper citation: RRID:Addgene_72676 Copy
Vector Backbone Description: Backbone Size:6798; Vector Backbone:see comments; Vector Types:Conditionally lethal; Bacterial Resistance:Streptomycin
Defining Citation: PMID:2160938
Comments: sacB was derived from pUM24 (Ried, J. L., and A. Colimer Gene 57:239-246, 1987) and from pUCD800 (Gay, et al. 1985 J. Bacteriol. 164:918-921).
The streptomycin/spectinomycin determinant in pRL277 was derived from the omega cassette (Prentki, et al. Gene 103: 17-23 1991).
Additional Reference for this plasmid:
Black TA, Cai Y, Wolk CP (1993) Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol Microbiol 9: 77-84. Publisher’s correction (1993): Mol Microbiol 10: 1153
Proper citation: RRID:Addgene_70255 Copy
Species: Homo sapiens
Genetic Insert: SUMO-1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:25007328
Proper citation: RRID:Addgene_52258 Copy
Species: Homo sapiens
Genetic Insert: SUMO-3
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:25007328
Proper citation: RRID:Addgene_52263 Copy
Species: Homo sapiens
Genetic Insert: SUMO-1
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:3781; Vector Backbone:pCDFDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:25007328
Proper citation: RRID:Addgene_52261 Copy
Vector Backbone Description: Backbone Size:5261; Vector Backbone:CloDF13; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39126322
Proper citation: RRID:Addgene_213132 Copy
Species: Synthetic
Genetic Insert: T0 terminator - Sm resistance cassette
Vector Backbone Description: Backbone Size:4948; Vector Backbone:pSH624-ssr; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:37798358
Proper citation: RRID:Addgene_198026 Copy
Species: E. coli
Genetic Insert: PPhlF-PBetI-YFP
Vector Backbone Description: Backbone Marker:Alec Nielsen; Backbone Size:3359; Vector Backbone:pAN4020; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:27034378
Proper citation: RRID:Addgene_74698 Copy
Genetic Insert: n/a
Vector Backbone Description: Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Streptomycin
Comments: This strain has 572 genetic changes compared to the DH10B reference genome in Genbank (CP000948.1). Please see https://www.ncbi.nlm.nih.gov/nuccore/CP110017 and the accompanying paper for more details.
Proper citation: RRID:Addgene_201049 Copy
Vector Backbone Description: Backbone Size:5007; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39244484
Comments: Low copy plasmid (pSC101 ori and repA), pUC19 MCS, confers streptomycin resistance
Proper citation: RRID:Addgene_225174 Copy
Vector Backbone Description: Backbone Size:4769; Vector Backbone:Unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin
Defining Citation: PMID:39244484
Comments: Low copy plasmid (pSC101 ori and repA), pUC18 MCS with FLAG epitope integrated at SmaI site, confers streptomycin resistance
Proper citation: RRID:Addgene_225165 Copy
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