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Species: E. coli
Genetic Insert: SoxS
Vector Backbone Description: Vector Backbone:p15A; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34800362
Proper citation: RRID:Addgene_177323 Copy
Species: E. coli
Genetic Insert: dxs
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:0; Vector Backbone:pBluescript; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11180061
Proper citation: RRID:Addgene_17821 Copy
Species: E. coli
Genetic Insert: FLIPrbs
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14550551
Comments: A RBP PCR product from genomic E. coli DNA encoding mature RBP was cloned into the KpnI site of FLIPglu-600u in pRSETB, replacing the glucose sensor's mglB moiety. The RBP sequence was found to carry a D208G substitution, which does not significantly affect the binding affinity.
Proper citation: RRID:Addgene_17860 Copy
Species: E. coli
Genetic Insert: FLIPrbs T135A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14550551
Comments: T135A was generated using QuikChange (Stratagene) in the mutant background of FLIPrib-250n.
Proper citation: RRID:Addgene_17861 Copy
Species: E. coli
Genetic Insert: FLIPrbs F16A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14550551
Comments: F16A was generated using QuikChange (Stratagene) in the mutant background of FLIPrib-250n.
Proper citation: RRID:Addgene_17864 Copy
Species: E. coli
Genetic Insert: FLIPrbs R141A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSET-B; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14550551
Comments: R141A was generated using QuikChange (Stratagene) in the mutant background of FLIPrib-250n.
Proper citation: RRID:Addgene_17865 Copy
Species: E. coli
Genetic Insert: FLII12Pglu-700uDelta6
Vector Backbone Description: Backbone Size:0; Vector Backbone:pEF/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18177733
Proper citation: RRID:Addgene_17867 Copy
Species: E. coli
Genetic Insert: OmpW
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pET11A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18641391
Proper citation: RRID:Addgene_112121 Copy
Species: E. coli
Genetic Insert: OmpT
Vector Backbone Description: Backbone Size:5636; Vector Backbone:pET11A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18641391
Proper citation: RRID:Addgene_112123 Copy
Species: E. Coli
Genetic Insert: MBP
Vector Backbone Description: Backbone Marker:Tobias Meyer (Addgene plasmid # 85132); Vector Backbone:pLV-EF1a-IRES-Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35972904
Proper citation: RRID:Addgene_175103 Copy
Species: E. coli
Genetic Insert: Catalytic domain of E.coli relA gene.
Vector Backbone Description: Backbone Marker:BglBrick; Vector Backbone:pBbS2k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34990598
Proper citation: RRID:Addgene_175595 Copy
Species: E. coli
Genetic Insert: Catalytic domain of E.coli relA gene.
Vector Backbone Description: Backbone Marker:BglBrick; Vector Backbone:pBbS2k; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34990598
Proper citation: RRID:Addgene_175590 Copy
Species: E. coli
Genetic Insert: inversion switch
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBAD18; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16534780
Proper citation: RRID:Addgene_17565 Copy
Species: E. coli
Genetic Insert: mCherry-eDHFR
Vector Backbone Description: Backbone Marker:Dr. Eugene Makeyev; Backbone Size:-1; Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33904910
Comments: Please note that there are minor differences between the Addgene NGS result and the depositor's sequence. These differences do not alter plasmid function.
Proper citation: RRID:Addgene_176201 Copy
Species: E. coli
Genetic Insert: RecT
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pTJV1Sc-rpoB; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:35673614
Proper citation: RRID:Addgene_166983 Copy
Species: E. coli
Genetic Insert: C-fragment of wtBlc
Vector Backbone Description: Backbone Size:4504; Vector Backbone:pMRBad; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33210733
Proper citation: RRID:Addgene_168257 Copy
Species: E. coli
Genetic Insert: Formaldehyde repressor protein (frmR)
Vector Backbone Description: Vector Backbone:pTR47m4; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28921510
Proper citation: RRID:Addgene_102436 Copy
Species: E. coli
Genetic Insert: Colicin E1 and E1 immunity protein
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19029376
Comments: Insert was cloned from ~base pair 5015 to ~500 of the circular ColE1 plasmid, where numbering begins within the colicin E1 gene. The ColE1 sequences include both the promoter for colicin E1 and for E1 immunity protein, which is transcribed in the opposite direction from the colicin gene. The cloned DNA does not include the intact kil (lysis protein) gene.
Proper citation: RRID:Addgene_103063 Copy
Species: E. coli
Genetic Insert: colicin E1 immunity protein
Vector Backbone Description: Backbone Marker:Novagen EMD/Millipore; Backbone Size:5627; Vector Backbone:pET52-b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27795317
Comments: The genes for colicin E1 with the R domain deletion and E1 immunity protein are transcribed from opposite strands of the vector. Colicin synthesis is under control of the vector inducible promoter; immunity protein synthesis is controlled by its own promoter.
Proper citation: RRID:Addgene_103065 Copy
Species: E. coli
Genetic Insert: HSP33 cys
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5190; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16054089
Comments: The HSP-FRET probe was generated by inserting YFP into the pECFP-N1 plasmid between the NheI and BglII sites and then ligating the redox-sensitive regulatory domain from the Escherichia coli HSP-33 between YFP and CFP via the EcoRI and BamHI sites. HSP-33: 210 amino acids; BA000007 region 4241962-4242826. Made in Hammerling lab; used in Waypa et al., Increases in mitochondrial reactive oxygen species trigger hypoxia-induced calcium responses in pulmonary artery smooth muscle cells, Circ Res. 2006 Oct 27; 99(9):970-8 PMID: 17008601.
Proper citation: RRID:Addgene_16076 Copy
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